Concept explainers
Interpretation:
The concentration of
Concept introduction:
Ammonium Sulfate or
Answer to Problem 5P
At the end of dialysis, the concentration is 0.001 M
Explanation of Solution
Given:
Precipitation of a protein with 1 M
1 ml of the sample is dialyzing in 1000 ml of buffer.
To find the concentration or volume of the concentrated or dilute solution the following equation is used:
The concentration of
Put the above values in equation:
Therefore, at the end of dialysis the concentration is 0.001 M
The process for further lowering the concentration of
The concentration could be further dropped by dialyzing the sample again. It can be done by placing the dialysis tube into a fresh 1000 mL solution (a buffer having no
So, it could be done by dialyzing the sample to 1mM, in a buffer-free solution of (NH4)2SO4.
At the end of dialysis, the concentration is 0.001 M
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Chapter 3 Solutions
Biochemistry
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