Biochemistry
9th Edition
ISBN: 9781319114671
Author: Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher: W. H. Freeman
expand_more
expand_more
format_list_bulleted
Concept explainers
Question
Chapter 3, Problem 8P
Interpretation Introduction
Interpretation:
The dependence of the sedimentation coefficient (s) of a spherical protein on its mass should be determined. The rate of sedimentation of 80-kDa protein than 40-kDa protein should be determined.
Concept introduction:
The sedimentation coefficient can be defined as a quantity associated with the microscopic particle size. It is equal to the particle’s terminal external velocity. It occurs when intermediate is centrifuged in a fluid divided by the centrifugal force acting on it. It is represented in units of time.
Expert Solution & Answer
Trending nowThis is a popular solution!
Students have asked these similar questions
Purification of a new unknown protein that you isolated from tissue and
Assume that you have reached the following data during the characterization;
Gel filtration: Gel filtration in protein native conformation
When chromatographed, it has a molecular weight of 240000 daltons (240
kDa) is detected to be around.
Gel filtration: The same protein is first denatured with 6 M guanidinium hydrochloride
subjected to gel filtration chromatography again under denatured conditions.
is retained, and the only column from the column with a molecular weight of about 60000 daltons (60 kDa)
a protein is obtained.
SDS-PAGE: Protein finally SDS-PAGE in the presence of beta-mercaptoethanol
(Sodium dodecyl-sulphate polyacrylamide gel electrophoresis) analysis
being held. As a result of SDS-PAGE analysis, their weight in the gel is approximately 40000 daltons.
Two protein bands corresponding to (40 kDa) and 20000 daltons (20 kDa)
is observed.
In the light of these findings, the quaternary/quaternary…
Disadvantages of cholesterol
resting state of the protein, the Lys 216 Schiff base has pKa = 9.5 and Asp 85 has pKa = 3.5. When the conformational change occurs, the proton that was on the Schiff base moves to Asp 85. This should tell you that one or both of these two pKas
changed with the conformational change. How does the pKa of Asp 85 compare with the pKa of the Lys 216 Schiff base after the conformational change?
Chapter 3 Solutions
Biochemistry
Ch. 3 - Prob. 1PCh. 3 - Prob. 2PCh. 3 - Prob. 3PCh. 3 - Prob. 4PCh. 3 - Prob. 5PCh. 3 - Prob. 6PCh. 3 - Prob. 7PCh. 3 - Prob. 8PCh. 3 - Prob. 9PCh. 3 - Prob. 10P
Ch. 3 - Prob. 11PCh. 3 - Prob. 12PCh. 3 - Prob. 13PCh. 3 - Prob. 14PCh. 3 - Prob. 15PCh. 3 - Prob. 16PCh. 3 - Prob. 17PCh. 3 - Prob. 18PCh. 3 - Prob. 19PCh. 3 - Prob. 20PCh. 3 - Prob. 21PCh. 3 - Prob. 22PCh. 3 - Prob. 23PCh. 3 - Prob. 24PCh. 3 - Prob. 25PCh. 3 - Prob. 26PCh. 3 - Prob. 27PCh. 3 - Prob. 28PCh. 3 - Prob. 29PCh. 3 - Prob. 30PCh. 3 - Prob. 31P
Knowledge Booster
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biochemistry and related others by exploring similar questions and additional content below.Similar questions
- Activity: Write the line structure of each of the following peptide at pH7 and identify how many peptide bond in each number. 1 Alanyl-phenylalanine 2. Lysyl-alanine 3.Phenylalanyl-tyrosyl-leucinearrow_forwardVertebrate proteins? What is meant by the term polypeptide backbone?arrow_forwardActivity: Write the line structure of each of the following peptide at pH7 and identify how many peptide bond in each number. 1. Glycyl-valyl-serine 2. Threonyl-cysteine 3. Isoleucyl-methionyl-aspartatearrow_forward
- Peptide bond properties. How these properties determine the three-dimensional structure of the protein.arrow_forwardstructure of cholesterol, define the three regions of the molecule, know that this molecule regulates membrane fluidity, and distinguish between esterified and unesterifiedcholesterolarrow_forwardHelp? Please explain salting out in protein purification.arrow_forward
- Choice and Preparation of a Buffer System1. Choosing the proper buffer solution In Protein Precipitation, two liters of 5mM buffer solution with pH 5.2 is needed in the isolation of albumin. Which among the following buffer solution is best fitted for said purpose? Justify your answer.Buffer solutions pKa Acetate buffer 4.73Tris- (hydroxymethy) aminomethane 8.08Phosphate buffer 7.20 2. Preparation of the chosen buffer system Calculate and measure the amounts (in grams if solid and in mL if liquid) of weak acid and conjugate base needed to be able to prepare the chosen buffer system in part A above. Express your answer in useful units (that is, prepare it from practical amounts or concentrations of starting materials).arrow_forwardNot the best with titration curves. Identify the position on the following diprotic titration drive where the amino acid has no net charge.arrow_forwardGABA (B) protein Need help answering questions about the GABA(B) protein. What is the primary structure Describe secondary structure (alpha-helix, beta sheet, and how many of each and what percent of the total protein)What is the tertiary structure (which should also show secondary structure)Does the protein have a quaternary structure, if so what is it?arrow_forward
- PRECIPITATION OF PROTEINS What are peptides? Do all proteins possess peptide bonds? Do all proteins respond to Biuret’s test? What is the difference between coagulation and denaturation? Why is egg white used as an antidote for lead and mercurial poisoning? Explain its mechanism of action. 4.Why is silver nitrate used in the cauterization of wound? Explain your answer.arrow_forwardProtein folding with PDI and Peptidyl-prolyl isomerasearrow_forwardMolecular detail of spike Y453Farrow_forward
arrow_back_ios
SEE MORE QUESTIONS
arrow_forward_ios
Recommended textbooks for you
- BiochemistryBiochemistryISBN:9781305577206Author:Reginald H. Garrett, Charles M. GrishamPublisher:Cengage Learning
Biochemistry
Biochemistry
ISBN:9781305577206
Author:Reginald H. Garrett, Charles M. Grisham
Publisher:Cengage Learning
Biomolecules - Protein - Amino acids; Author: Tutorials Point (India) Ltd.;https://www.youtube.com/watch?v=ySNVPDHJ0ek;License: Standard YouTube License, CC-BY