Biochemistry
Biochemistry
6th Edition
ISBN: 9781305577206
Author: Reginald H. Garrett, Charles M. Grisham
Publisher: Cengage Learning
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Chapter 30, Problem 12P
Interpretation Introduction

Interpretation:

The method of identifying whether a methionyl-tRNA initiates the synthesis of protein or delivers a Met residue to incorporate in the polypeptide chain by bacterial cells needs to be determined. The difference in the Met codon for the two purposes needs to be explained. The role of eukaryotic cells in handling such problems needs to be explained.

Concept Introduction:

The polypeptide chains are formed from the amino acids joining together by peptide bonds. The proteins are known to be linear polymers formed due to the bond formation between alpha-carboxylic group of the single amino acid and alpha-amino group of the other via peptide bond.

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Question:- Fill the Blank! In bacteria, the ___consensus sequence of mRNA binds to the ____rRNA of the 30S small subunit during translation initiation, while in eukaryotes, the _______ consensus sequence of mRNA contains the_____.
2a) In prokaryotes, a small ribosomal subunit can potentially get on an mRNA anywhere it can find enough space to do so. Once a small ribosomal subunit has bound to an mRNA, it will scan along that mRNA in the 5' to 3' direction looking for a start codon at which to initiate translation. How does the small ribosomal subunit distinguish a start codon from any other AUG codon that simply codes for methionine in the middle of a coding sequence?
From this overall anticodon sequence in tRNA, 3'-CAUCGGAAUAGAUCGCUAGUGGCAGGCAUAAUGAUCACCGGUCUGAGAAAAGUGGUACAUAUCAAC-5' Using ONE-letter amino acid code starting from N-terminus to C-terminus,  what is the amino acid sequence that will be coded for?
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