In the bloodstream of dog 2.00mL of solution was injected which has 0.113 microcurie per milliliter of tritium. The counting rate of 1.00mL sample of blood after allowing time of homogenization is 14.9 counts per seconds. The blood volume of animal is to be determined.
The volume of blood of animal is calculated by the isotope dilution method which is given as-
= mass of inactive sample
= counting rate
= counting rate of inactive analyte
= isolated mass which is purified from the mixture of radioactive tracer
= mass of radioactive tracer
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