In the bloodstream of dog 2.00mL of solution was injected which has 0.113 microcurie per milliliter of tritium. The counting rate of 1.00mL sample of blood after allowing time of homogenization is 14.9 counts per seconds. The blood volume of animal is to be determined. Concept introduction: The volume of blood of animal is calculated by the isotope dilution method which is given as- m x = R t R m × m m − m t Where, m x = mass of inactive sample R t = counting rate R m = counting rate of inactive analyte m m = isolated mass which is purified from the mixture of radioactive tracer m t = mass of radioactive tracer

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Principles of Instrumental Analysis

7th Edition
Douglas A. Skoog + 2 others
Publisher: Cengage Learning
ISBN: 9781305577213
BuyFind

Principles of Instrumental Analysis

7th Edition
Douglas A. Skoog + 2 others
Publisher: Cengage Learning
ISBN: 9781305577213

Solutions

Chapter 32, Problem 32.14QAP
Interpretation Introduction

Interpretation:

In the bloodstream of dog 2.00mL of solution was injected which has 0.113 microcurie per milliliter of tritium. The counting rate of 1.00mL sample of blood after allowing time of homogenization is 14.9 counts per seconds. The blood volume of animal is to be determined.

Concept introduction:

The volume of blood of animal is calculated by the isotope dilution method which is given as-

mx=RtRm×mmmt

Where,

mx = mass of inactive sample

Rt = counting rate

Rm = counting rate of inactive analyte

mm = isolated mass which is purified from the mixture of radioactive tracer

mt = mass of radioactive tracer

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