# (a) Interpretation: The amount of protein sample hydrolyzed is 10.0g. The specific activity of the 14 C labeled threonine is 1000cpm/mg and the 3.00mg of threonine is added to the hydrolysate. After mixing the specific activity of the threonine is 20cpm/mg when the pure threonine of amount 11.5 mg was isolated. The percentage of protein which is threonine is to be determined. Concept introduction: The formula for the half time is given as- t 1 / 2 = 0.693 λ ………………….. (1) Where, λ = d e c a y c o n s t a n t The amount of inactive species is calculated by the isotope dilution method which is given as- m x = R t R m × m m − m t ………………………. (1) Here, m x = mass of inactive sample R t = counting rate R m = counting rate of inactive analyte m m = isolated mass which is purified from the mixture of radioactive tracer m t = mass of radioactive tracer

### Principles of Instrumental Analysis

7th Edition
Douglas A. Skoog + 2 others
Publisher: Cengage Learning
ISBN: 9781305577213

### Principles of Instrumental Analysis

7th Edition
Douglas A. Skoog + 2 others
Publisher: Cengage Learning
ISBN: 9781305577213

#### Solutions

Chapter 32, Problem 32.17QAP
Interpretation Introduction

## (a)Interpretation:The amount of protein sample hydrolyzed is 10.0g. The specific activity of the 14C labeled threonine is 1000cpm/mg and the 3.00mg of threonine is added to the hydrolysate. After mixing the specific activity of the threonine is 20cpm/mg when the pure threonine of amount 11.5 mg was isolated. The percentage of protein which is threonine is to be determined.Concept introduction:The formula for the half time is given as-t1/2=0.693λ ………………….. (1)Where,λ=decay constantThe amount of inactive species is calculated by the isotope dilution method which is given as-mx=RtRm×mm−mt ………………………. (1)Here,mx = mass of inactive sampleRt = counting rateRm = counting rate of inactive analytemm = isolated mass which is purified from the mixture of radioactive tracermt = mass of radioactive tracer

Interpretation Introduction

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