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ChemistryPrinciples of Instrumental Analysis(a) Interpretation: The amount of protein sample hydrolyzed is 10.0g. The specific activity of the 14 C labeled threonine is 1000cpm/mg and the 3.00mg of threonine is added to the hydrolysate. After mixing the specific activity of the threonine is 20cpm/mg when the pure threonine of amount 11.5 mg was isolated. The percentage of protein which is threonine is to be determined. Concept introduction: The formula for the half time is given as- t 1 / 2 = 0.693 λ ………………….. (1) Where, λ = d e c a y c o n s t a n t The amount of inactive species is calculated by the isotope dilution method which is given as- m x = R t R m × m m − m t ………………………. (1) Here, m x = mass of inactive sample R t = counting rate R m = counting rate of inactive analyte m m = isolated mass which is purified from the mixture of radioactive tracer m t = mass of radioactive tracerStart your trial now! First week only $4.99!*arrow_forward*

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7th Edition

Douglas A. Skoog + 2 others

Publisher: Cengage Learning

ISBN: 9781305577213

Chapter 32, Problem 32.17QAP

Interpretation Introduction

**(a)**

**Interpretation:**

The amount of protein sample hydrolyzed is 10.0g. The specific activity of the

**Concept introduction:**

The formula for the half time is given as-

Where,

The amount of inactive species is calculated by the isotope dilution method which is given as-

Here,

Interpretation Introduction

**(b)**

**Interpretation:**

The amount of protein sample hydrolyzed is 10.0g. The specific activity of the

**Concept introduction:**

The formula for the half time is given as-

Where,

The specific activity of radioactive element is calculated by the following equation-

Or

Where,

A_{0} = initial activity

A = final activity

The amount of threonine is calculated by the following formula-

Where,