Genetic Analysis: An Integrated Approach (3rd Edition)
Genetic Analysis: An Integrated Approach (3rd Edition)
3rd Edition
ISBN: 9780134605173
Author: Mark F. Sanders, John L. Bowman
Publisher: PEARSON
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Chapter 4, Problem 3P

When working on barley plants, two researchers independently identify a short-plant mutation and develop homozygous recessive lines of short plants. Careful measurements of the height of mutant short plants versus normal tall plants indicate that the two mutant lines have the same height. How would you determine if these two mutant lines carry mutation of the same gene or of different genes?

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A mutation that breaks which of the following genes would be most likely to produce theanthocyanless phenotype of the green-stem Wisconsin Fast Plants (meaning no purple stems):PAL, CHS, C3H, FLS, or DFR? Explain your reasoning.
In the early 1990s, Carolyn Napoli and her colleagues were working on petunias, attempting to genetically engineer a variety with dark purple petals by introducing numerous copies of a gene that encodes purple pigment in the flower petals . Their thinking was that extra copies of the gene would cause more purple pigment to be produced and would result in a petunia with an even darker hue of purple. However, much to their surprise, many of the plants carrying extra copies of the purple gene were completely white or had only patches of color. Molecular analysis revealed that the amount of mRNA produced by the purple gene was reduced 50-fold in the engineered plants compared with wild-type plants. Somehow, the introduction of extra copies of the purple gene silenced both the introduced copies and the plant’s own purple genes. Provide a possible explanation for how the introduction of numerous copies of the purple gene silenced all copies of the purple gene.
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Genetic Analysis: An Integrated Approach (3rd Edition)

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