Pearson eText Genetic Analysis: An Integrated Approach -- Instant Access (Pearson+)
3rd Edition
ISBN: 9780135564172
Author: Mark Sanders, John Bowman
Publisher: PEARSON+
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Textbook Question
Chapter 7, Problem 14P
Bacterial DNA polymerase I and DNA polymerase III per-form different functions during
a. Identify the principal functions of each molecule.
b. If mutation inactivated DNA polymerase I in a strain of E. coli, would the cell be able to replicate its DNA? If so, what kind of abnormalities would you expect to find in the cell?
c. If a strain of E. coli acquired a mutation that inactivated DNA polymerase III function, would the cell be able to replicate its DNA? Why or why not?
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A. Diagram a short single strand of DNA 5’ -AA-GG- 3’. Show the chemical structure of the phosphoribosyl backbone and the attachment point for nucleotides added as “A” or “G”.
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In Semi conservative replication:
A. After one round of replication of a single molecule of DNA, one DNA molecule will be produced that contains two parental strands of DNA and one DNA molecule will be
produced that contains two new (or de novo) strands.
B. After one round of replication of a single molecule of DNA, two resulting DNA molecules will be produced both of which contain a mix of both parental and new
DNA interspersed on every strand of DNA
C. After two rounds of replication of a single molecule of DNA, two resulting DNA molecules will contain both a parental strand and a new strand of DNA and the other
two resulting DNA molecules will contain all new (or de novo) DNA
D. After two rounds of replication of a single molecule of DNA, one resulting DNA molecule will contain 2 parental strands of DNA and the other three resulting DNA
molecules will contain all new (or de novo) DNA
E. A and C
F. B and D
Chapter 7 Solutions
Pearson eText Genetic Analysis: An Integrated Approach -- Instant Access (Pearson+)
Ch. 7 - What results from the experiments of Frederick...Ch. 7 - 7.2 Explain why Avery, MacLeod, and McCarty’s in...Ch. 7 - 7.3 Hershey and Chase selected the bacteriophage...Ch. 7 - 7.4 Explain how the Hershey and Chase experiment...Ch. 7 - 7.5 One strand of a fragment of duplex DNA has the...Ch. 7 - 7.6 The principles of complementary base pairing...Ch. 7 - For the following fragment of DNA, determine the...Ch. 7 - 7.8 Figures present simplified depictions of...Ch. 7 - 7.9 Consider the sequence -ACGCTACGTC-.
What is...Ch. 7 - DNA polymerase III is the main DNA-synthesizing...
Ch. 7 - There is a problem completing the replication of...Ch. 7 - Explain how RNA participates in DNA replication.Ch. 7 - A sample of double-stranded DNA is found to...Ch. 7 - Bacterial DNA polymerase I and DNA polymerase III...Ch. 7 - Diagram a replication fork in bacterial DNA and...Ch. 7 - Prob. 16PCh. 7 - Which of the following equalities is not true for...Ch. 7 - List the order in which the following proteins and...Ch. 7 - Two viral genomes are sequenced, and the following...Ch. 7 - Matthew Meselson and Franklin Stahl demonstrated...Ch. 7 - Raymond Rodriguez and colleagues demonstrated...Ch. 7 - 7.22 Joel Huberman and Arthur Riggs used pulse...Ch. 7 - 7.23 Why do the genomes of eukaryotes, such as...Ch. 7 - Bloom syndrome (OMIM 210900) is an autosomal...Ch. 7 - 7.25 How does rolling circle replication (see...Ch. 7 - Telomeres are found at the ends of eukaryotic...Ch. 7 - A family consisting of a mother (I-1), a father...Ch. 7 - In a dideoxy DNA sequencing experiment, four...Ch. 7 - Prob. 29PCh. 7 - Using an illustration style and labeling similar...Ch. 7 - A PCR reaction begins with one double-stranded...Ch. 7 - Prob. 32PCh. 7 - Prob. 33PCh. 7 - 7.34 A sufficient amount of a small DNA fragment...Ch. 7 - You are participating in a study group preparing...Ch. 7 - Prob. 36PCh. 7 - The following diagram shows the parental strands...Ch. 7 - Go to the OMIM website...
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Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.Similar questions
- Below is a study of a colony of cells, determine that some of these cells have a mutated DNA polymerase I that results in loss of function of this enzyme. - What will the effect of the mutation in DNA polymerase I be on DNA replication? Include leading and lagging strand - Will this mutation in DNA polymerase I have an impact on another step in DNA replication? Will DNA be replicatation be impacted? Are any enzymes involved?arrow_forwarda. What process would be unaffected in with defective topoisomerase? Prokaryotic DNA packaging Eukaryotic DNA packaging Prokaryotic DNA replication Eukaryotic DNA replication All of the above rely on topoisomerase function b. Why is primase required for DNA replication? To unwind the DNA helix To prime nucleotides for addition to the growing chain To provide a 3' OH for DNA polymerase To recognize the origin of replication To remove supercoils ahead of the replication machinery C. What is the purpose of PCR reaction? To alter the sequence of a fragment of DNA. To insert a fragment of DNA into a bacterium To amplify a fragment of ONA To destroy a fragment of DNA To determine the sequence of nucleotides in a fragment of DNA.arrow_forwardPlease consider figures 1 and 2 below. 5' A B 5' -I H. D. Figure 1: Replication Figure 2: Transcription a. Which letters, A to J represent DNA strands that will be synthesized continuously? b. At which letter, A to J, are ribonucleotides being added? c. Name and describe the process that occurs in prokaryotes as the synthesized mRNA hangs free of the double helix while transcription continues. d. In what direction will the replication bubble move in figure 1 as replication continues? Rleace Ibol ancwors (a) to (d)arrow_forward
- You examine DNA replication in an E. coli mutant, which has a partially defective DNA polymerase. In vitro experiments using the mutant DNA polymerase gives an error rate of 10-3, as compared to the expected error rate of 10-6. Which of the following activities is the mutant polymerase likely to be missing, as compared to the normal polymerase? Explain your answer.arrow_forwardThe experiment below is from a seminal set of experiments in the 1960s that illustrated the role of various repair pathways for DNA damage caused by UV radiation. In this experiment, the scientists isolated E coli strains that are mutant in the Rec A gene, the UvrA gene or both. They then irradiated cultures of each strain with increasing doses of UV light and measured the effect on cell viability. Answer the following questions about this data. A. Which DNA repair pathway and repair activity is inhibited by the Rec A mutant? B. Which DNA repair pathway and repair function is inhibited by UvrA mutant? C. Why is the UvrA/RecA double mutant so much more senitive to UV light than either mutant alone?arrow_forwardIndicate whether each of the following statements is true or false. If a statement is false, explain why it is false. A. The repair polymerase is the enzyme that proofreads the newly synthesized strands to ensure the accuracy of DNA replication. B. There is a single enzyme that degrades the RNA primers and lays down the corresponding DNA sequence behind it. C. DNA ligase is required to seal the sugar-phosphate backbone between all the DNA fragments on the lagging strand. D. The repair polymerase does not require the aid of the sliding clamp, because it is only synthesizing DNA over very short stretches. Answer the following questions about DNA replication. On a DNA strand that is being synthesized, which end is growing the 3' end, the 5' end, or both ends? Explain your answer. А. B. On a DNA strand that is being used as a template, where is the copying occurring relative to the replication origin-3' of the origin, 5', or both?arrow_forward
- You are studying a colony of cells and determine that some of these cells have a mutated DNA polymerase I that results in loss of function of this enzyme. A) What will the effect of the mutation in DNA polymerase I be on DNA replication? In your answer make sure to describe what would be observed in the leading and lagging strand and explain your reasoning. B) Will this mutation in DNA polymerase I have an impact on another step in DNA replication? In your answer make sure to indicate whether DNA replication will be impacted or not. If it is not, explain why. If it is impacted, then describe the step that is impacted and name the molecule or enzyme involved.arrow_forwardGenerate a concept map that includes all the specifics below: Classification based on: 1. their effect on the DNA 2. on their phenotypic effect. Kinds of DNA damage and lesions (spontaneous and induced) produced by specific exposures. (Note: don't forget transposons and CRISPR Cas9) Specific repair mechanisms that fix each kind of DNA damage or lesion (during/post replication). Include information about the number of replication cycles for a dominant mutation to cause a phenotype. And the number of cycles for a recessive mutation to possibly cause a phenotype.arrow_forwardMatch the enzymes provided from (1-4) in the list of choices with their matching function (A-D) during DNA replication. A. Disrupts hydrogen bonds between DNA bases B. Can only add nucleotides to an existing 3 OH end C. Can't add nucleotides to a chain, but can make covalent bonds D. Actually a specialized form of RNA polymerase select 1. DNA polymerase select 2. Primase select 3. Ligase select v 4. Helicasearrow_forward
- 5) In DNA replication, which of the following events happens during both leading and lagging strand synthesis? a. RNA primers help initiate DNA synthesis. b. Topoisomerase separates complementary strands of DNA. c. Ligase joins together multiple Okazaki fragments. d. DNA polymerase synthesizes a single, continuous strand of DNA. Explain WHY? 6) Suppose you are provided with an actively dividing culture of E. coli bacteria to which radioactive thymine has been added. What would happen if a cell replicates once in the presence of this radioactive base? a. One of the daughter cells, but not the other, would have radioactive DNA. b. Neither of the two daughter cells would be radioactive. c. All four bases of the DNA would be radioactive. d. DNA in both daughter cells would be radioactive. Explain WHY?arrow_forwardConsider a DNA molecule with the following structure a. if during replication , the T at position 4 is read as an A resulting in a misincorporation in the new DNA strand . If the misincorporation is not repair , draw or write the results of the second round of replication b. After the second round of replication , what specific type of point mutation is now permanent in the DNA sequence ? c. List the two cellular mechanisms that failed to prevent the establishment of this permanent mutation .arrow_forwarda. Using nucleotide letters, show the kind of cut that could be made on a DNA molecule to circularize it into a plasmid. b. What are restriction length polymorphisms, and how are they used?arrow_forward
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