Microbiology: An Evolving Science (Fourth Edition)
4th Edition
ISBN: 9780393615098
Author: John W. Foster, Joan L. Slonczewski
Publisher: W. W. Norton & Company
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Chapter 7.6, Problem 2TQ
Summary Introduction
To review:
The possibility of transferring a foreign gene into the bacteria which possess endonuclease restriction enzyme.
Introduction:
The enzyme that breaks the DNA (deoxyribonucleic acid) into segments by making cuts at specific restriction or recognition sites is the restriction enzyme. These enzymes are also known as endonuclease enzymes that break the phosphodiester bond in the DNA molecules.
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In cloning human DNA, why is it necessary to insert the DNA into a vector such as a bacterial plasmid?
Why are nucleic acid probes necessary to lyse cells containing plasmids in order to detect the product of the cloned gene?
Why do bacteria make restriction endonucleases? What is it about the endonucleases that prevents bacteria from destroying their own DNA?
Chapter 7 Solutions
Microbiology: An Evolving Science (Fourth Edition)
Ch. 7.2 - Prob. 1TQCh. 7.2 - Prob. 2TQCh. 7.2 - Prob. 3TQCh. 7.2 - Prob. 4TQCh. 7.3 - Prob. 1TQCh. 7.3 - Prob. 2TQCh. 7.3 - Prob. 3TQCh. 7.3 - Prob. 4TQCh. 7.3 - Prob. 5TQCh. 7.6 - Prob. 1TQ
Ch. 7.6 - Prob. 2TQCh. 7.6 - Prob. 3TQCh. 7 - Prob. 1RQCh. 7 - Prob. 2RQCh. 7 - Prob. 3RQCh. 7 - Prob. 4RQCh. 7 - Prob. 5RQCh. 7 - Prob. 6RQCh. 7 - Prob. 7RQCh. 7 - Prob. 8RQCh. 7 - Prob. 9RQCh. 7 - Prob. 10RQCh. 7 - Prob. 11RQCh. 7 - Prob. 12RQCh. 7 - Prob. 13RQCh. 7 - Prob. 14RQCh. 7 - Prob. 15RQCh. 7 - Prob. 1TQCh. 7 - Prob. 2TQCh. 7 - Prob. 3TQ
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- If restriction endonucleases are produced by bacteria within a host, why don’t these enzymes chew up the genomic DNA of their host? What is the role of DNA methyltransferase in this?arrow_forwardWhy do restriction endonucleases not hydrolyze DNA from the organism that produces it?arrow_forwardWhy must the gene be inserted into a vector for it to be cloned?arrow_forward
- What feature is commonly seen in the sequences recognized by type II restriction enzymes?arrow_forwardConsidering that prokaryote genomes do not have large introns, how is it possible to move a eukaryotic gene into a transformed bacterium, since they lack a spliceosome?arrow_forwardWhy does reversible chain termination sequencing yield short reads?arrow_forward
- In producing genetically engineered human insulin in bacteria, why is it important to use the samerestriction enzyme to cut both the human DNA and the bacterial plasmid?arrow_forwardWhy is it necessary to use only the Klenow fragment, rather than intact E. coli Pol I, in DNA sequencing reactions?arrow_forwardIf restriction endonucleases are produced by bacteria within a host, why don’t these enzymes chew up the genomic DNA of their host? What is the role of DNA methyltransferase in this? Indicate the answerarrow_forward
- How does the use of restriction endonucleases of different specificities aid in the sequencing of DNA?arrow_forwardWhich restriction enzyme is best suited for cloning in pUC8?arrow_forwardWhat is the significance of palindromic Restriction Endonuclease recognition and sequence overhangs after digestion?arrow_forward
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