BIOCHEM-ACHIEVE(FIRST DAY DISCOUNTED)
9th Edition
ISBN: 2818000069358
Author: BERG
Publisher: MAC HIGHER
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Chapter 9, Problem 6P
Interpretation Introduction
Interpretation:
Whether the increased rate of endonucleases enzymes is beneficial to host cells or not is to be stated.
Concept introduction:
The restriction enzymes are also called as restriction endonucleases. The restriction endonucleases are used for the hydrolysis of the phosphodiester backbone present on the particular DNA sequence.
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A classic way to isolate thymidylate synthase–negative mutants of bacteriais to treat a growing culture with thymidine and trimethoprim. Most ofthe cells are killed, and the survivors are greatly enriched in thymidylatesynthase–negative mutants.(a) What phenotype would allow you to identify these mutants?(b) What is the biochemical rationale for the selection? (That is, why are themutants not killed under these conditions?)(c) How would the procedure need to be modified to select mammalian cellmutants defective in thymidylate synthase?
Consider the biochemical pathway shown here. Suppose that a strain of bacteria must synthesize compound 4 to survive and divide. Successful survival and division of bacteria is observed as growth of colonies on an agar plate. This strain of bacteria can grow colonies on minimal medium as long as it is supplemented with compound 1. You are in a lab that has isolated several mutants of this strain. You find that these mutants cannot grow on minimal medium supplemented with compound 1, though they can grow colonies if supplemented with compound 4. Considering what you know about the Beadle-Tatum experiments, which of the following statements would be one that should be true?
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