Biochemistry
9th Edition
ISBN: 9781319114671
Author: Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher: W. H. Freeman
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Chapter 9, Problem 6P
Interpretation Introduction
Interpretation:
Whether the increased rate of endonucleases enzymes is beneficial to host cells or not is to be stated.
Concept introduction:
The restriction enzymes are also called as restriction endonucleases. The restriction endonucleases are used for the hydrolysis of the phosphodiester backbone present on the particular DNA sequence.
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Mutants that are lacY- retain the capacity to synthesizeβ-galactosidase. However, even though the lacI gene isstill intact, β-galactosidase can no longer be induced byadding lactose to the medium. Explain
Regulation of Genes and Their products
1. Given the following genotypes, explain how the mutation (identified by a (-) superscript) wil affect E. coll grown in lactose medium. Will the lac operon be on or off? Will there be a complete set of gene products from the lac operon? What will be the implication of the missing gene product, if ever? Will the cell be able to survive in the lactose medium or not?
a. I+p+o+z- y+
b. i- p+o+z+y+
c. i+p+o- z+y+
d. i+p- o+z+y+
2. In terms of the trp operon, differentiate between two normal bacterial cultures, one grown in a medium supplied with tryptophan and the other medium without tryptophan.
3. Experiments show that mutations at gene E lead to non-repressible transcription of trp genes. Why?
BIOCHEM : Draw the chemical mechanism (with electron-pushing arrows) for the addition of an RNA nucleotide triphosphate to a growing mRNA transcript. Be sure to explicitly show the attacking nucleophile and the leaving group.
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