Biochemistry
9th Edition
ISBN: 9781319114671
Author: Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher: W. H. Freeman
expand_more
expand_more
format_list_bulleted
Concept explainers
Question
Chapter 9, Problem 3P
Interpretation Introduction
Interpretation:
Whether the given reduction in activity during the mutation of Ser
Concept introduction:
Mutation is defined as the change in the sequence/order of DNA bases. It occurs due to the introduction of radiation, chemicals, or an internal disorder. Mutation may be caused by a single change in base sequence.
Subtilisin refers to a protein-digesting enzyme. It initiates the attack of nucleophile on the amide bond by a serine residue.
Expert Solution & Answer
Want to see the full answer?
Check out a sample textbook solutionStudents have asked these similar questions
Explain the biochemical defect that leads to Lesch-Nyhan syndrome and suggest how the defect might cause the behavioral symptoms characteristic of the disorder.
prpc is a normal protein of our neuronal cell membrane that is sensitive to protease action (ie can be broken down by protease) true or false?
1G. Please help me in detail. For Molecular Mechanism of ATP versus GTP selectivity of adenylate kinase,
Write an expression for the reaction velocity.
Knowledge Booster
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biochemistry and related others by exploring similar questions and additional content below.Similar questions
- MULTIPLE CHOICE In patients with Lesch Nyhan Syndrome, purine nucleotides are overproduced and over excreted. The hypoxanthine analogue Allopurinol, which effectively treats gout , has no effect on the severe neurological symptoms of Lesch- Nyhan patients because it does not A. decrease de novo pyrimidine synthesis B. decrease de novo purine synthesis C. decrease urate synthesis D. increase PRPP levels (Phosphoribosyl pyrophosphate)arrow_forwardNeed help, please. Draw an oxygen binding curve of Hb at a pH of 7.4 and another curve where all 2,3 BPG has been removed Draw an oxygen binding curve of Hb at a pH of 7.4 and another at pH. Draw an oxygen binding curve of Hb at a pH of 7.4 and another with a mutant Hb in which the predominant form of the protein is monomericarrow_forwardprovide examples of the levels of protein structure for mevalonate kinase (2HFU) provide the levels of protein structure (primary, secondary, tertiary, etc.) for mevalonate kinase (2HFU)arrow_forward
- Human insulin is prodụced as a recombinant protein in the yeast Saccharomyces cerevisiae The protein is extracted and purified for the treatment of diabetes -a $32 billion industry! For efficacy, insulin must be modified by the addition of oligosaccharyl (sugar) groups If you were a scientist working on this project, how would you isolate the glycosylated insulin from the un- glycosylated forms?arrow_forwardRefer to the molecular structure of Molecule 1 (attached image). This molecule forms a dimer via pi interactions, resulting to effective molecular stacking. If the molecule can inhibit toxic effects at 0.5mM against gastric carcinoma cells, does it mean that the dimer of this nucleoside analogue will exhibit an IC50 of about 0.25mM?arrow_forward8. Match each of the following amino acids with the intermediate needed for its synthesis.(a) 3-Phosphoglycerate 1. glutamate 2. serine 3. asparagine (b) oxaloacetate 1. glutamate 2. serine 3. Asparaginearrow_forward
- 1F. Please help me in detail. for molecular Mechanism of ATP versus GTP selectivity of adenylate kinase, Draw a reaction scheme that includes both the substrate and the inhibitor under study here. Be sure to label KI, Ks, kparrow_forwardWhy Phenylmethylsulfonyl fluoride (PMSF, shown in Fig.) does not inhibit aspartate protease as potent as serine protease?arrow_forwardWhat peptides are expected to be produced when α-melanotropin is cleaved by (a) trypsin, (b) cyanogen bromide, or (c) thermolysin? (as shown)arrow_forward
- Serine protease enzyme mutation To show differences in the effect of the nucleophilic attack of the carbonyl group (C=O) of peptide bond between the catalytic triad of serine, histidine and aspartic acid, and another catalytic triad contains alanine, histidine and aspartic acid Provide/ draw an example of catalytic mechanism with catalytic triad contains alanine, histidine and aspartic Please answer completely will give rating surelyarrow_forwardHydrolysis of β-endorphin (a peptide containing 31 amino acid residues) produces the following amino acids: Tyr 1), Gly 3), Phe 2), Met, Thr 3), Ser 2), Lys 5), Gin 2), Pro, Leu 2), Val 2), Asn 2), Ala 2), lle, His, and Glu Treatment with carboxypeptidase liberates Gln. Treatment with DNFB liberates DNP-Tyr. Treatment with trypsin produces the following peptides: Lys, Gly—Gln, Asn—Ala—He—Val—Lys, Tyr—Gly—Gly—Phe—Met—Thr—Ser—Glu—Lys, Asn—Ala—His—Lys, Ser—Gln—Thr—Pro—Leu— Val—Thr—Leu—Phe—Lys Treatment with chymotrypsin produces the following peptides: Lys—Asn—Ala—He—Val—Lys—Asn—Ala—His—Lys—Lys—Gly—Gln Tyr—Gly—Gly—Phe Met—Thr—Ser—Glu—Lys—Ser—Gin—Thr—Pro—Leu—Val—Thr—Leu—Phe What is the primary sequence of β-endorphin?arrow_forwardYou have an unknown heptapeptide that gives the following amino acids after acid hydrolysis (2M.2K,R, A,P) treatment with CNBr gives hormoserine lactone (M*), a tetrapeptide (2K,M*,A) and (P,R). Treatment with trypsin gives free P, free K, (A,K,M) and (R,M). what is the sequence of amino acids for the heptapeptide? show work.arrow_forward
arrow_back_ios
SEE MORE QUESTIONS
arrow_forward_ios
Recommended textbooks for you
- BiochemistryBiochemistryISBN:9781305577206Author:Reginald H. Garrett, Charles M. GrishamPublisher:Cengage Learning
Biochemistry
Biochemistry
ISBN:9781305577206
Author:Reginald H. Garrett, Charles M. Grisham
Publisher:Cengage Learning
Metabolic Pathways; Author: Wisc-Online;https://www.youtube.com/watch?v=m61bQYio9ys;License: Standard Youtube License