Microbiology: An Evolving Science (Fourth Edition)
4th Edition
ISBN: 9780393615098
Author: John W. Foster, Joan L. Slonczewski
Publisher: W. W. Norton & Company
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Chapter 9.1, Problem 1TQ
Summary Introduction
To review:
The ability of a mutant of streptococcus lacking ComD to transform DNA.
Introduction:
Transformation is the process of introduction or transfer of DNA (deoxyribonucleic acid) into the bacterial cell from the outside environment. There are various methods for the transformation of DNA in a particular cell. The transformasome proteins are also associated with the transformation of DNA. To utilize the DNA, the cell has to activate the competent factor (CF) which binds to the ComD and enters into the cell. The Com is the sensory protein present in the cell membrane.
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Draw a diagram/figure to explain the conjugation process (. You should include in the diagram the F- recipient, Hfr Donor and the transconjugant/recombinant recipient. Make sure to include the genes encoding for Leucine, Threonine, Thiamine and Streptomycin resistance in your diagram. How does an Hfr strain of coli transfers chromosomal DNA to an F- strain? What determines how much of the chromosomal DNA is transferred?
Bacterial transformation involves DNA transfer to a recipient cell
Group of answer choices
as naked DNA in solution
by a bacteriophage
by cell-to-cell contact
by sexual reproduction
Review the Process Diagram, Figure 8.9, and answer this question.The type of horizontal gene transfer that involves a physical connection between two bacterial cells is
transduction.
expression.
conjugation.
transformation.
recombination.
Chapter 9 Solutions
Microbiology: An Evolving Science (Fourth Edition)
Ch. 9.1 - Prob. 1TQCh. 9.1 - Prob. 2TQCh. 9.1 - Prob. 3TQCh. 9.1 - Prob. 4TQCh. 9.2 - Prob. 1TQCh. 9.3 - Prob. 1TQCh. 9.4 - Prob. 1TQCh. 9.4 - Prob. 2TQCh. 9.5 - Prob. 1TQCh. 9.6 - Prob. 1TQ
Ch. 9 - Prob. 1RQCh. 9 - Prob. 2RQCh. 9 - Prob. 3RQCh. 9 - Prob. 4RQCh. 9 - Prob. 5RQCh. 9 - Prob. 6RQCh. 9 - Prob. 7RQCh. 9 - Prob. 8RQCh. 9 - Prob. 9RQCh. 9 - Prob. 10RQCh. 9 - Prob. 11RQCh. 9 - Prob. 12RQCh. 9 - Prob. 13RQCh. 9 - Prob. 14RQCh. 9 - Prob. 15RQCh. 9 - Prob. 16RQCh. 9 - Prob. 1TQCh. 9 - Prob. 2TQCh. 9 - Prob. 3TQCh. 9 - Prob. 4TQCh. 9 - Prob. 5TQCh. 9 - Prob. 6TQ
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Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.Similar questions
- Draw a diagram/figure to explain the conjugation process (e.g. use PowerPoint or draw one by hand and include a photo of it). You should include in the diagram the F- recipient, Hfr Donor and the transconjugant/recombinant recipient. Make sure to include the genes encoding for Leucine, Threonine, Thiamine and Streptomycin resistance in your diagram. How does an Hfr strain of coli transfers chromosomal DNA to an F- strain? What determines how much of the chromosomal DNA is transferred?arrow_forwardBacterial transformation is a major concern in many medical settings. Why might health care providers be concerned? a. Pathogenic bacteria could introduce diseasecausing genes in non-pathogenic bacteria. b. Antibiotic resistance genes could be introduced to new bacteria to create “superbugs.” c. Bacteriophages could spread DNA encoding toxins to new bacteria. d. All of the above.arrow_forwardWhen a bacteriophage accidentally incorporates a fragment of host DNA in its capsid and delivers it to another (living) bacterial cell, the process is called _____. transformation transduction conjugation speciationarrow_forward
- A student mixes some heat-killed type IIS Streptococcus pneumoniae bacteria with live type IIR bacteria and injects the mixture into a mouse. The mouse develops pneumonia and dies. The student recovers some type IIS bacteria from the dead mouse. If this is the only experiment conducted by the student, has the student demonstrated that transformation has taken place? What other explanations might explain the presence of the type IIS bacteria in the dead mouse?arrow_forwardDefine the following terms: Bacteriophage λ DNA Plasmid Maparrow_forwardA patient has a Haemophilus influenzae respiratory infection. She is prescribed Amoxicillin. Unfortunately, this strain of H. influenzae has resistance to this antibiotic. Her symptoms worsen. When she again visits the doctor, a nasty Amoxicillin resistant Streptococcus is isolated from her nasal passages. What is the most likely explanation? transduction. conjugation. transformation. none of the abovearrow_forward
- True or False: Plasmids can ONLY be used in transforming bacterial cells.arrow_forwarda bacteriophage has been isolated from the growth chambers that has evolved the capability to the methylate its own DNA when it is replicated inside the host bacterium. why is this a problem?arrow_forwardThe flask where you grow the E. coli culture got exposed to UV rays, explain what kind of DNA damage can happen and how bacteria will repair the damage. Describe two DNA repair pathways.arrow_forward
- What is the correct order for the steps of transformation given inthe following list?1. Recombination with the bacterial chromosome2. Binding of a large DNA fragment to the surface of a bacterialcell3. Cutting a large DNA fragment into smaller pieces4. Uptake of DNA into the cytoplasm5. Degradation of one of the DNA strandsa. 1, 2, 3, 4, 5b. 2, 3, 5, 4, 1c. 2, 3, 4, 5, 1d. 2, 5, 4, 3, 1arrow_forward. When a virus mistakenly picks up a segment of host bacterial DNA, packages it into a viral particle, then transfers it to a new bacterial host cell, this transfer of DNA is properly called: transduction transformation conjugation transversion transitionarrow_forwardImagine that you are a student in Alfred Hershey and Martha Chase’s lab in the late 1940s. You are given five test tubes containing E. Coli bacteria infected with T2 bacteriophages that have been labeled with either 32P or 35S. Unfortunately, you forget to mark the tubes and are now uncertain about which tubes is which. You performed their blender experiment and got the following results. Which tube out of these 5 contains E. Coli infected with 32P-labeled phage? Explain your answer.arrow_forward
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