Gram staining

“earth. METHODS AND MATERIALS MATERIALS 1 Becker 1 Nutrient agar plate 1 pair plastic gloves 1 Potato agar plate 1 Staining tray 100ml of distilled water 2 MacConkey Agar Plates 3 Blank slides 3 plastic cups 6 pipettes 95% Grams alcohol Bibulous paper Boat & Scale Bunsen Burner & Flint Crystal-violet Grams Iodine Incubator of 35⁰ Celsius (silver) Inoculating loop Microscope Pond water Safranin Soil Tap water Timer

This project is intended to push us to use the knowledge we have acquired, using proper staining techniques and thorough understanding of testing techniques and results to draw conclusions. Conclusion: The first two tests performed required that I streak the liquid culture onto a TSA plate in order to isolate individual colonies. The second involved performing a successful gram stain, which resulted in gram positive staphylococci. After successfully performing these tests I was able to narrow down

question has few symptoms related to this bacterium. The fever, raised his body temperature to 103 ºF and, the respiratory rate of 36 per minute which was caused by pneumonia. Klebsiella pneumoniae is a gram negative bacterium so gram staining of the sputum would be a good place to eliminate all the gram positive bacterium. However it has been reported this test has a

physiological class of bacteria capable of utilising agar as a sole carbon source. This ability is made available by the use of agarases - enzymes which break down agarose into oligosaccharides. This physiological class branches through genii, regardless of Gram Stain status or morphology. Through a review of scientific literature we can find identification methods, optimum conditions and the general function and location of agarolytic bacteria, as well as methods to culture them in vitro for study and experimentation

line on each slant, I incubated it at 37oC for 24 hours. The next day, I made sure my slants were pure and also there was a thick line of organism where I had inoculated with a yellow-white color. Now I was ready to begin making my slides and start staining. I used E. Coli and S. Aureus next to my unknown to help me

consisted of an unknown gram positive or gram negative bacteria. A sterile technique was performed by the lab manual instructions which is stated in the references.

An 18-year old female has been diagnosed as having a sexually transmitted disease due to Neisseria gonorrhoea; Gonorrhoea is a sexually transmitted infection (STI) caused by bacteria called Neisseria gonorrhoeae or gonococcus. Gonorrhoea is pathogenic bacterial type of infection.Itis a rather common infectious bacterium that can grow and rapidly multiply in the mucous membranes, in areas such as the mouth, throat and anus of males as well as females. Cervix, fallopian tubes and the uterus of the

series of biochemical tests were conducted in order to determine the identity of an unknown gram negative bacterium. The unknown had the potential to be one of six different gram negative bacteria: Escherichia coli, Pseudomonas aeruginosa, Enterobacter aerogenes, Proteus mirabilis, Klebsiella pneumonia, or Salmonella typhirmurium. After using the T-streak method to isolate pure colonies and confirming the gram negative nature of the unknown bacterium the unknown was subjected to seven biochemical tests

The genus Streptococcus is classified as a gram positive bacteria because of the spherical or ovoid cells that often arranged in pairs and the color it turns too after undergoing the gram staining technique. Unlike the catalase positive genus Staphylococcus, Streptococcus is catalase negative (Fox). Many Streptococcus bacteria are known to be facultative anaerobes, meaning they prefer to grow in places that have no oxygen but will adjust to places that contain some oxygen (Holt, et al.). On the contrary

arrived at the answer is stated. A short paragraph explaining how the results are presented can be included. Example: Unknown G had the following morphology on a TSA plate: medium sized opaque cream colored colony. After determining that it was a Gram negative rod, an oxidase test was performed and it was inoculated into a BCP lactose tube