. (a) Explain the biochemical basis for the fact that one can synchro-nize cell populations þy treating them with deoxythymidine.(b) Explain the apparent paradox that dATP at low concentrations isan activator of ribonucleotide reductase, whereas at higher concen-trations it becomes inhibitory.

Cell synchronization is a technique where different cells in culture at different phases of the cell…

Answered: . (a) Explain the biochemical basis for…

Biochemistry

6th Edition

ISBN:9781305577206

Author:Reginald H. Garrett, Charles M. Grisham

Publisher:Reginald H. Garrett, Charles M. Grisham

Chapter26: Synthesis And Degradation Of Nucleotides

Section: Chapter Questions

Problem 6P: Allosteric Regulation of Ribonucleotide Reductase by ATP and Deoxynucleotides Describe the...

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Similar questions

Allosteric Regulation of Ribonucleotide Reductase by ATP and Deoxynucleotides Describe the underlying rationale for the regulatory effects exerted on ribonucleotide reductase by ATP, dATP, dTTP, and dGTP.
In the serine protease triad, the proximity of an aspartatecarboxylate group to the imidazole group of histidine raisesthe latter’s pKa. Explain.
Briefly describe the induced-fit conformational change when hexokinase binds its substrate.
From the complete oxidation of glucose (glucose → 6CO2), how many total nucleotide triphosphates are yielded (be sure to deduct payback) as part of substrate level phosphorylation?
Explain activation-induced deaminase (AID) induced mutation in the DNA arose by deamination of Cytosine (C) to Uracil (U) in the DNA.
Tissues that are unable to produce nucleotides de novo depend on ____________________ to maintain an adequate supply of adenine nucleotides. (A) the redox reactions carried out by ribonucleotide reductase (B) the action of PRPP amidotransferase(C) uptake of ATP through dietary intake(D) salvaging hypoxanthine using HGPRT (E) the action of xanthine oxidase
Provide a simple sketch of the covalent intermediate likely to form between active site serine residue and diphenylcarbamyl chloride. Is DPCC more likely to covalently bind to serine, aspartate, or histidine?
Describe how the concentrations of the four deoxyribonucleotides are balanced by ribonucleotide reductase.
BIOCHEMISTRY Why does a dual layer of Cr/Au (20/100nm) delaminate during a GSTAT electrochemical polymerization of PEDOT:PSS?
. In Figure 6-11,a. in view of the position of HPA oxidase earlier in thepathway compared to that of HA oxidase, would youexpect people with tyrosinosis to show symptoms ofalkaptonuria?b. if a double mutant could be found, would you expecttyrosinosis to be epistatic to alkaptonuria?
Lysozyme is an enzyme that hydrolyzes bacterial cell wall polysaccharides. When this reaction is carried out in the presence of H218O, it is observed that there is retention of configuration at the C1 carbon of the D site sugar as shown below: Would this result suggest that the enzyme mechanism involves the direct nucleophilic attack of water at C1 or that the enzyme mechanism involves attack by a nucleophilic amino acid side chain of the enzyme that generates a transient covalent intermediate?

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. (a) Explain the biochemical basis for the fact that one can synchro- nize cell populations þy treating them with deoxythymidine. (b) Explain the apparent paradox that dATP at low concentrations is an activator of ribonucleotide reductase, whereas at higher concen- trations it becomes inhibitory.