Suppose your supervisor is working on a molecule “X” with UniProtKB accession number P18564. Being a team member in the project, you have been asked to provide the following information with high accuracy? I. Chromosome number: II. Protein size: III. Number of exons
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- A scientist wants to clone a molecule bearing UniProtKB accession number P43657. How will he come to know about the following? I. Exact genomic location of the molecule under investigation II. Coding and noncoding segments of the molecule III. Untranslated regions of the moleculeWrite the detail or exact information of the follwoing of molecule “X” with UniProtKB accession number P18564. I. Chromosome number:II. Protein size:III. Number of exons:IV. Stop codon:V. Size of the longest exon in nucleotidesWhat are the detail or exact information of the following of molecule “X” with UniProtKB accession number P18564. I. Chromosome number:II. Protein size:III. Number of exons:IV. Stop codon:V. Size of the longest exon in nucleotides
- Shown below is an R loop prepared for electron microscopy by annealing a purified eukaryotic messenger RNA with DNA from a genomic clone containing the full-length gene corresponding to the mRNA. (a) How many exons does the gene contain? How many introns? (b) Where in this structure would you expect to find a 5′,5′-internucleotide bond? Where would you expect to find a polyadenylic acid sequence?A scientist wants to clone a molecule uniProtKB accession number P43657. How will be come to know about the following? . Exact genomic location of the molecule under investigation . Coding and noncoding segments of the molecule . Untranslated regions of the moleculeIn reversible terminator sequencing, how would the sequencing process be affected if the 3′-end-blocking group of each nucleotide were replaced with the 3′-H present in the dideoxynucleotides used in Sanger sequencing?
- Below is a sequence of DNA.5'-ttaccgataattctctctcccctcttccatgattctgattaaagaaggcgagaacgaaactatttgttaatacc-3' How many "reading frames" can be identified for this sequence? How many "open reading frames" can be identified for this sequence? What is the frame of the longest ORF? How many codons are in the longest ORF? What is the frame of the shortest ORF? How many AA are in the shortest ORF? Using the one letter code for Amino Acids, what is the predicted AA sequence of the shortestORF (from N to C-terminal end)? Using the one letter code for Amino Acids, what is the predicted AA sequence of the longestORF (from N to C-terminal end)?For the following five sequences, what is the consensus sequence? 5′–GGGAGCG–3′ 5′–GAGAGCG–3′ 5′–GAGTGCG–3′ 5′–GAGAACG–3′ 5′–GAGAGCA–3′ a. 5′–GGGAGCG–3′ b. 5′–GAGAGCG–3′ c. 5′–GAGTGCG–3′ d. 5′–GAGAACG–3′Below is a sequence of 540 bases from a genome. What information would you use to find the beginnings and ends of open reading frames? How many open reading frames can you find in this sequence? Which open reading frame is likely to represent a protein- coding sequence, and why? Which are probably not functioning protein-coding sequences, and why? Note: for simplicitys sake, analyze only this one strand of the DNA double helix, reading from left to right, so you will only be analyzing three of the six reading frames shown in Figure 19.4.
- Below is a sequence of DNA. 5'-ttaccgataattctctctcccctcttccatgattctgattaaagaaggcgagaacgaaactatttgttaatacc-3' How many codons are in the longest ORF? What is the frame of the shortest ORF? How many AA are in the shortest ORF?How many possible open reading frames (frameswithout stop codons) exist that extend through the following sequence?5′... CTTACAGTTTATTGATACGGAGAAGG...3′3′... GAATGTCAAATAACTATGCCTCTTCC...5'What is EcoRI? How does EcoRI differ from an exonuclease?