Genetics: From Genes to Genomes
Genetics: From Genes to Genomes
6th Edition
ISBN: 9781259700903
Author: Leland Hartwell Dr., Michael L. Goldberg Professor Dr., Janice Fischer, Leroy Hood Dr.
Publisher: McGraw-Hill Education
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Chapter 10, Problem 20P

In the human immune system, so-called B cells can make more than a billion different types of antibody molecules that protect us from infection. However, our genomes have only three genes that encode the polypeptides found in antibodies. What experiments could you perform to determine what kind of combinatorial events occur at the DNA level (V-D-J joining) and RNA level (alternative splicing) for any of these genes?

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According to the scenario shown, how many segments of DNA (one, two, or three) are removed during site-specific recombination within the gene that encodes the κ (kappa) light chain for IgG proteins? How many segments are spliced out of the pre-mRNA?
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