Genetics: From Genes to Genomes
Genetics: From Genes to Genomes
6th Edition
ISBN: 9781259700903
Author: Leland Hartwell Dr., Michael L. Goldberg Professor Dr., Janice Fischer, Leroy Hood Dr.
Publisher: McGraw-Hill Education
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Chapter 10, Problem 24P
Summary Introduction

a.

To determine:

The human gene that best matches the query sequence and observes only at the first entry, which corresponds to the first entry under the descriptions.

Introduction:

The genome is the complete DNA set present in the organism. BLAST is a bioinformatic tool used in the analysis of data obtained from DNA sequencing.

Summary Introduction

b.

To determine:

The exact match and to choose “mouse genomic + transcript” as the database to search.

Introduction:

The match between the sequence of two different organisms shows the evolutionary relationships between them.

Summary Introduction

c.

To determine:

The gene conserved in the mouse and the way in which it is conserved.

Introduction:

The gene which is same throughout evolution are the conserved genes. The conserved gene is unique.

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If you have access to the necessary computer software, make asequence file and analyze it in the following ways: What is thetranslated sequence in all three reading frames? What is the longest open reading frame? Is the sequence homologous to any known sequences? If so, does this provide any clues about the function of the sequence?
Below is a sequence of DNA.   5'-ttaccgataattctctctcccctcttccatgattctgattaaagaaggcgagaacgaaactatttgttaatacc-3'   How many "reading frames" can be identified for this sequence?  How many "open reading frames" can be identified for this sequence? What is the frame of the longest ORF?
In a genome project, the following genomic DNA sequences were obtained. Assemble the sequences into a contig. Using the assembled sequence, perform a BLASTn search. Does the search produce sequences similar to your assembled sequence? 5’ TCGGGGTCCTGGGATCTCATCACTGCAGCGC 3’ 5’ACTGCAGCGCTTTCCCAGCGGGCGGTGGTAC 3’ 5’GGGCGGTGGTACTCGGGAAGTCAGGAGTGTT 3’ 5’AGGAGTGTTTAAAACCTGGGGACTGGTTTTG 3’ 5’TGGTTTTGGGGGCGCTGAAGGCAGCGCAGGA 3’
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