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Analyzing Cloned Sequences A new gene that causes an inherited form of retinal degeneration has been cloned from mice. This disease leads to blindness in affected individuals. As a researcher in a human vision laboratory, you want to see if a similar gene exists in humans (genes with similar sequences usually mean similar functions). With your knowledge of the Southern blot procedure, how would you go about doing this? Start with the isolation of human DNA.

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Human Heredity: Principles and Iss...

11th Edition
Michael Cummings
Publisher: Cengage Learning
ISBN: 9781305251052

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BuyFindarrow_forward

Human Heredity: Principles and Iss...

11th Edition
Michael Cummings
Publisher: Cengage Learning
ISBN: 9781305251052
Chapter 13, Problem 19QP
Textbook Problem
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Analyzing Cloned Sequences

A new gene that causes an inherited form of retinal degeneration has been cloned from mice. This disease leads to blindness in affected individuals. As a researcher in a human vision laboratory, you want to see if a similar gene exists in humans (genes with similar sequences usually mean similar functions). With your knowledge of the Southern blot procedure, how would you go about doing this? Start with the isolation of human DNA.

Summary Introduction

To describe: The steps to find out the presence of a gene in humans that can cause the retinal degeneration by using the procedure of Southern blotting.

Introduction: A method for transferring DNA fragments from a gel to a membrane filter and screened with probes to identify a fragment of interest is called “southern blotting.”

The fragment of interest can be used in hybridization experiments.

Explanation of Solution

The steps in the procedure of Southern blotting for the identification of the presence of specific gene are given below:

  • • Human DNA is isolated from the cell containing the gene of interest.
  • • DNA is sliced into fragments by using restriction enzymes.
  • • Separation of DNA segments take place
  • • The DNA fragments are separated based on their size by the process of gel electrophoresis...

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