(a) Interpretation: The reason for a “smear” in lane 1 of the western blot gel should be explained. Concept introduction: Glycogenin is an enzyme which catalyzes the formation of short glycogen primers from glucose molecules. Alpha amylase catalyzes the hydrolysis of alpha linkages in polysaccharides and release glucose and maltose. SDS-PAGE is a biochemical technique used to separate charged molecules from a mixture, according to their molecular masses. SDS acts as a surfactant and negatively charge the proteins evenly. Thus, separating them only based on their molecular mass. Western blot is visualizing technique which is commonly used for analyzing proteins. Proteins separated by SDS-PAGE electrophoresis is transferred to a membrane, so that it can be visualized easily with the specific antibodies against the target protein.

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Answer 1

Question

Chapter 21, Problem 44P

Interpretation Introduction

(a)

Interpretation:

The reason for a “smear” in lane 1 of the western blot gel should be explained.

Concept introduction:

Glycogenin is an enzyme which catalyzes the formation of short glycogen primers from glucose molecules.

Alpha amylase catalyzes the hydrolysis of alpha linkages in polysaccharides and release glucose and maltose.

SDS-PAGE is a biochemical technique used to separate charged molecules from a mixture, according to their molecular masses. SDS acts as a surfactant and negatively charge the proteins evenly. Thus, separating them only based on their molecular mass.

Western blot is visualizing technique which is commonly used for analyzing proteins. Proteins separated by SDS-PAGE electrophoresis is transferred to a membrane, so that it can be visualized easily with the specific antibodies against the target protein.

Interpretation Introduction

(b)

Interpretation:

The significance of the decrease in high molecular weight bands in lane 2 should be explained.

Concept introduction:

Glycogenin is an enzyme which catalyzes the formation of short glycogen primers from glucose molecules.

Alpha amylase catalyzes the hydrolysis of alpha linkages in polysaccharides and release glucose and maltose.

SDS-PAGE is a biochemical technique used to separate charged molecules from a mixture, according to their molecular masses. SDS acts as a surfactant and negatively charge the proteins evenly. Thus, separating them only the basis of their molecular mass.

Western blot is a visualizing technique which is commonly used for analyzing proteins. Proteins separated by SDS-PAGE electrophoresis are transferred to a membrane, so that it can be visualized easily with the specific antibodies against the target protein.

Interpretation Introduction

(c)

Interpretation:

Significance of the difference between lane 2 and 3 should be explained

Concept introduction:

Glycogenin is an enzyme which catalyzes the formation of short glycogen primers from glucose molecules.

Alpha amylase catalyzes the hydrolysis of alpha linkages in polysaccharides and release glucose and maltose.

SDS-PAGE is a biochemical technique used to separate charged molecules from a mixture, according to their molecular masses. SDS acts as a surfactant and negatively charge the proteins evenly. Thus, separating them only based on their molecular mass.

Western blot is a visualizing technique which is commonly used for analyzing proteins. Proteins separated by SDS-PAGE electrophoresis is transferred to a membrane, so that it can be visualized easily with the specific antibodies against the target protein.

Interpretation Introduction

(d)

Interpretation:

A plausible reason for no difference between lanes 3 and 4 should be suggested.

Concept introduction:

Glycogenin is an enzyme which catalyzes the formation of short glycogen primers from glucose molecules.

Alpha amylase catalyzes the hydrolysis of alpha linkages in polysaccharides and release glucose and maltose.

SDS-PAGE is a biochemical technique used to separate charged molecules from a mixture, according to their molecular masses. SDS acts as a surfactant and charges the proteins both negatively and evenly. Thus, separating them only based on their molecular mass.

Western blot is a visualizing technique which is commonly used for analyzing proteins. Proteins separated by SDS-PAGE electrophoresis are transferred to a membrane, so that it can be visualized easily with the specific antibodies against the target protein.

Interpretation Introduction

(e)

Interpretation:

The 66 kDabands observed in all the lanes treated with amylase, despite the fact that the cells were treated differently should be justified.

Concept introduction:

Glycogenin is an enzyme which catalyzes the formation of short glycogen primers from glucose molecules.

Alpha amylase catalyzes the hydrolysis of alpha linkages in polysaccharides and release glucose and maltose.

SDS-PAGE is a biochemical technique used to separate charged molecules from a mixture, according to their molecular masses. SDS acts as a surfactant and charges the proteins both negatively and evenly. Thus, separating them only based on their molecular mass.

Western blot is a visualizing technique which is commonly used for analyzing proteins. Proteins separated by SDS-PAGE electrophoresis are transferred to a membrane, so that it can be visualized easily with the specific antibodies against the target protein.

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Answer 2

Question

Chapter 21, Problem 44P

Interpretation Introduction

(a)

Interpretation:

The reason for a “smear” in lane 1 of the western blot gel should be explained.

Concept introduction:

Glycogenin is an enzyme which catalyzes the formation of short glycogen primers from glucose molecules.

Alpha amylase catalyzes the hydrolysis of alpha linkages in polysaccharides and release glucose and maltose.

SDS-PAGE is a biochemical technique used to separate charged molecules from a mixture, according to their molecular masses. SDS acts as a surfactant and negatively charge the proteins evenly. Thus, separating them only based on their molecular mass.

Western blot is visualizing technique which is commonly used for analyzing proteins. Proteins separated by SDS-PAGE electrophoresis is transferred to a membrane, so that it can be visualized easily with the specific antibodies against the target protein.

Interpretation Introduction

(b)

Interpretation:

The significance of the decrease in high molecular weight bands in lane 2 should be explained.

Concept introduction:

Glycogenin is an enzyme which catalyzes the formation of short glycogen primers from glucose molecules.

Alpha amylase catalyzes the hydrolysis of alpha linkages in polysaccharides and release glucose and maltose.

SDS-PAGE is a biochemical technique used to separate charged molecules from a mixture, according to their molecular masses. SDS acts as a surfactant and negatively charge the proteins evenly. Thus, separating them only the basis of their molecular mass.

Western blot is a visualizing technique which is commonly used for analyzing proteins. Proteins separated by SDS-PAGE electrophoresis are transferred to a membrane, so that it can be visualized easily with the specific antibodies against the target protein.

Interpretation Introduction

(c)

Interpretation:

Significance of the difference between lane 2 and 3 should be explained

Concept introduction:

Glycogenin is an enzyme which catalyzes the formation of short glycogen primers from glucose molecules.

Alpha amylase catalyzes the hydrolysis of alpha linkages in polysaccharides and release glucose and maltose.

SDS-PAGE is a biochemical technique used to separate charged molecules from a mixture, according to their molecular masses. SDS acts as a surfactant and negatively charge the proteins evenly. Thus, separating them only based on their molecular mass.

Western blot is a visualizing technique which is commonly used for analyzing proteins. Proteins separated by SDS-PAGE electrophoresis is transferred to a membrane, so that it can be visualized easily with the specific antibodies against the target protein.

Interpretation Introduction

(d)

Interpretation:

A plausible reason for no difference between lanes 3 and 4 should be suggested.

Concept introduction:

Glycogenin is an enzyme which catalyzes the formation of short glycogen primers from glucose molecules.

Alpha amylase catalyzes the hydrolysis of alpha linkages in polysaccharides and release glucose and maltose.

SDS-PAGE is a biochemical technique used to separate charged molecules from a mixture, according to their molecular masses. SDS acts as a surfactant and charges the proteins both negatively and evenly. Thus, separating them only based on their molecular mass.

Western blot is a visualizing technique which is commonly used for analyzing proteins. Proteins separated by SDS-PAGE electrophoresis are transferred to a membrane, so that it can be visualized easily with the specific antibodies against the target protein.

Interpretation Introduction

(e)

Interpretation:

The 66 kDabands observed in all the lanes treated with amylase, despite the fact that the cells were treated differently should be justified.

Concept introduction:

Glycogenin is an enzyme which catalyzes the formation of short glycogen primers from glucose molecules.

Alpha amylase catalyzes the hydrolysis of alpha linkages in polysaccharides and release glucose and maltose.

SDS-PAGE is a biochemical technique used to separate charged molecules from a mixture, according to their molecular masses. SDS acts as a surfactant and charges the proteins both negatively and evenly. Thus, separating them only based on their molecular mass.

Western blot is a visualizing technique which is commonly used for analyzing proteins. Proteins separated by SDS-PAGE electrophoresis are transferred to a membrane, so that it can be visualized easily with the specific antibodies against the target protein.

Expert Solution & Answer

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Check out a sample textbook solution

See solution

Answer 3

Question

Chapter 21, Problem 44P

Interpretation Introduction

(a)

Interpretation:

The reason for a “smear” in lane 1 of the western blot gel should be explained.

Concept introduction:

Glycogenin is an enzyme which catalyzes the formation of short glycogen primers from glucose molecules.

Alpha amylase catalyzes the hydrolysis of alpha linkages in polysaccharides and release glucose and maltose.

SDS-PAGE is a biochemical technique used to separate charged molecules from a mixture, according to their molecular masses. SDS acts as a surfactant and negatively charge the proteins evenly. Thus, separating them only based on their molecular mass.

Western blot is visualizing technique which is commonly used for analyzing proteins. Proteins separated by SDS-PAGE electrophoresis is transferred to a membrane, so that it can be visualized easily with the specific antibodies against the target protein.

Interpretation Introduction

(b)

Interpretation:

The significance of the decrease in high molecular weight bands in lane 2 should be explained.

Concept introduction:

Glycogenin is an enzyme which catalyzes the formation of short glycogen primers from glucose molecules.

Alpha amylase catalyzes the hydrolysis of alpha linkages in polysaccharides and release glucose and maltose.

SDS-PAGE is a biochemical technique used to separate charged molecules from a mixture, according to their molecular masses. SDS acts as a surfactant and negatively charge the proteins evenly. Thus, separating them only the basis of their molecular mass.

Western blot is a visualizing technique which is commonly used for analyzing proteins. Proteins separated by SDS-PAGE electrophoresis are transferred to a membrane, so that it can be visualized easily with the specific antibodies against the target protein.

Interpretation Introduction

(c)

Interpretation:

Significance of the difference between lane 2 and 3 should be explained

Concept introduction:

Glycogenin is an enzyme which catalyzes the formation of short glycogen primers from glucose molecules.

Alpha amylase catalyzes the hydrolysis of alpha linkages in polysaccharides and release glucose and maltose.

SDS-PAGE is a biochemical technique used to separate charged molecules from a mixture, according to their molecular masses. SDS acts as a surfactant and negatively charge the proteins evenly. Thus, separating them only based on their molecular mass.

Western blot is a visualizing technique which is commonly used for analyzing proteins. Proteins separated by SDS-PAGE electrophoresis is transferred to a membrane, so that it can be visualized easily with the specific antibodies against the target protein.

Interpretation Introduction

(d)

Interpretation:

A plausible reason for no difference between lanes 3 and 4 should be suggested.

Concept introduction:

Glycogenin is an enzyme which catalyzes the formation of short glycogen primers from glucose molecules.

Alpha amylase catalyzes the hydrolysis of alpha linkages in polysaccharides and release glucose and maltose.

SDS-PAGE is a biochemical technique used to separate charged molecules from a mixture, according to their molecular masses. SDS acts as a surfactant and charges the proteins both negatively and evenly. Thus, separating them only based on their molecular mass.

Western blot is a visualizing technique which is commonly used for analyzing proteins. Proteins separated by SDS-PAGE electrophoresis are transferred to a membrane, so that it can be visualized easily with the specific antibodies against the target protein.

Interpretation Introduction

(e)

Interpretation:

The 66 kDabands observed in all the lanes treated with amylase, despite the fact that the cells were treated differently should be justified.

Concept introduction:

Glycogenin is an enzyme which catalyzes the formation of short glycogen primers from glucose molecules.

Alpha amylase catalyzes the hydrolysis of alpha linkages in polysaccharides and release glucose and maltose.

SDS-PAGE is a biochemical technique used to separate charged molecules from a mixture, according to their molecular masses. SDS acts as a surfactant and charges the proteins both negatively and evenly. Thus, separating them only based on their molecular mass.

Western blot is a visualizing technique which is commonly used for analyzing proteins. Proteins separated by SDS-PAGE electrophoresis are transferred to a membrane, so that it can be visualized easily with the specific antibodies against the target protein.

Expert Solution & Answer

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Check out a sample textbook solution

See solution

Answer 4

Question

Chapter 21, Problem 44P

Interpretation Introduction

(a)

Interpretation:

The reason for a “smear” in lane 1 of the western blot gel should be explained.

Concept introduction:

Glycogenin is an enzyme which catalyzes the formation of short glycogen primers from glucose molecules.

Alpha amylase catalyzes the hydrolysis of alpha linkages in polysaccharides and release glucose and maltose.

SDS-PAGE is a biochemical technique used to separate charged molecules from a mixture, according to their molecular masses. SDS acts as a surfactant and negatively charge the proteins evenly. Thus, separating them only based on their molecular mass.

Western blot is visualizing technique which is commonly used for analyzing proteins. Proteins separated by SDS-PAGE electrophoresis is transferred to a membrane, so that it can be visualized easily with the specific antibodies against the target protein.

Interpretation Introduction

(b)

Interpretation:

The significance of the decrease in high molecular weight bands in lane 2 should be explained.

Concept introduction:

Glycogenin is an enzyme which catalyzes the formation of short glycogen primers from glucose molecules.

Alpha amylase catalyzes the hydrolysis of alpha linkages in polysaccharides and release glucose and maltose.

SDS-PAGE is a biochemical technique used to separate charged molecules from a mixture, according to their molecular masses. SDS acts as a surfactant and negatively charge the proteins evenly. Thus, separating them only the basis of their molecular mass.

Western blot is a visualizing technique which is commonly used for analyzing proteins. Proteins separated by SDS-PAGE electrophoresis are transferred to a membrane, so that it can be visualized easily with the specific antibodies against the target protein.

Interpretation Introduction

(c)

Interpretation:

Significance of the difference between lane 2 and 3 should be explained

Concept introduction:

Glycogenin is an enzyme which catalyzes the formation of short glycogen primers from glucose molecules.

Alpha amylase catalyzes the hydrolysis of alpha linkages in polysaccharides and release glucose and maltose.

SDS-PAGE is a biochemical technique used to separate charged molecules from a mixture, according to their molecular masses. SDS acts as a surfactant and negatively charge the proteins evenly. Thus, separating them only based on their molecular mass.

Western blot is a visualizing technique which is commonly used for analyzing proteins. Proteins separated by SDS-PAGE electrophoresis is transferred to a membrane, so that it can be visualized easily with the specific antibodies against the target protein.

Interpretation Introduction

(d)

Interpretation:

A plausible reason for no difference between lanes 3 and 4 should be suggested.

Concept introduction:

Glycogenin is an enzyme which catalyzes the formation of short glycogen primers from glucose molecules.

Alpha amylase catalyzes the hydrolysis of alpha linkages in polysaccharides and release glucose and maltose.

SDS-PAGE is a biochemical technique used to separate charged molecules from a mixture, according to their molecular masses. SDS acts as a surfactant and charges the proteins both negatively and evenly. Thus, separating them only based on their molecular mass.

Western blot is a visualizing technique which is commonly used for analyzing proteins. Proteins separated by SDS-PAGE electrophoresis are transferred to a membrane, so that it can be visualized easily with the specific antibodies against the target protein.

Interpretation Introduction

(e)

Interpretation:

The 66 kDabands observed in all the lanes treated with amylase, despite the fact that the cells were treated differently should be justified.

Concept introduction:

Glycogenin is an enzyme which catalyzes the formation of short glycogen primers from glucose molecules.

Alpha amylase catalyzes the hydrolysis of alpha linkages in polysaccharides and release glucose and maltose.

SDS-PAGE is a biochemical technique used to separate charged molecules from a mixture, according to their molecular masses. SDS acts as a surfactant and charges the proteins both negatively and evenly. Thus, separating them only based on their molecular mass.

Western blot is a visualizing technique which is commonly used for analyzing proteins. Proteins separated by SDS-PAGE electrophoresis are transferred to a membrane, so that it can be visualized easily with the specific antibodies against the target protein.

Expert Solution & Answer

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Check out a sample textbook solution

See solution

Answer 5

Chapter 21, Problem 44P

Interpretation Introduction

(a)

Interpretation:

The reason for a “smear” in lane 1 of the western blot gel should be explained.

Concept introduction:

Glycogenin is an enzyme which catalyzes the formation of short glycogen primers from glucose molecules.

Alpha amylase catalyzes the hydrolysis of alpha linkages in polysaccharides and release glucose and maltose.

SDS-PAGE is a biochemical technique used to separate charged molecules from a mixture, according to their molecular masses. SDS acts as a surfactant and negatively charge the proteins evenly. Thus, separating them only based on their molecular mass.

Western blot is visualizing technique which is commonly used for analyzing proteins. Proteins separated by SDS-PAGE electrophoresis is transferred to a membrane, so that it can be visualized easily with the specific antibodies against the target protein.

Interpretation Introduction

(b)

Interpretation:

The significance of the decrease in high molecular weight bands in lane 2 should be explained.

Concept introduction:

Glycogenin is an enzyme which catalyzes the formation of short glycogen primers from glucose molecules.

Alpha amylase catalyzes the hydrolysis of alpha linkages in polysaccharides and release glucose and maltose.

SDS-PAGE is a biochemical technique used to separate charged molecules from a mixture, according to their molecular masses. SDS acts as a surfactant and negatively charge the proteins evenly. Thus, separating them only the basis of their molecular mass.

Western blot is a visualizing technique which is commonly used for analyzing proteins. Proteins separated by SDS-PAGE electrophoresis are transferred to a membrane, so that it can be visualized easily with the specific antibodies against the target protein.

Interpretation Introduction

(c)

Interpretation:

Significance of the difference between lane 2 and 3 should be explained

Concept introduction:

Glycogenin is an enzyme which catalyzes the formation of short glycogen primers from glucose molecules.

Alpha amylase catalyzes the hydrolysis of alpha linkages in polysaccharides and release glucose and maltose.

SDS-PAGE is a biochemical technique used to separate charged molecules from a mixture, according to their molecular masses. SDS acts as a surfactant and negatively charge the proteins evenly. Thus, separating them only based on their molecular mass.

Western blot is a visualizing technique which is commonly used for analyzing proteins. Proteins separated by SDS-PAGE electrophoresis is transferred to a membrane, so that it can be visualized easily with the specific antibodies against the target protein.

Interpretation Introduction

(d)

Interpretation:

A plausible reason for no difference between lanes 3 and 4 should be suggested.

Concept introduction:

Glycogenin is an enzyme which catalyzes the formation of short glycogen primers from glucose molecules.

Alpha amylase catalyzes the hydrolysis of alpha linkages in polysaccharides and release glucose and maltose.

SDS-PAGE is a biochemical technique used to separate charged molecules from a mixture, according to their molecular masses. SDS acts as a surfactant and charges the proteins both negatively and evenly. Thus, separating them only based on their molecular mass.

Western blot is a visualizing technique which is commonly used for analyzing proteins. Proteins separated by SDS-PAGE electrophoresis are transferred to a membrane, so that it can be visualized easily with the specific antibodies against the target protein.

Interpretation Introduction

(e)

Interpretation:

The 66 kDabands observed in all the lanes treated with amylase, despite the fact that the cells were treated differently should be justified.

Concept introduction:

Glycogenin is an enzyme which catalyzes the formation of short glycogen primers from glucose molecules.

Alpha amylase catalyzes the hydrolysis of alpha linkages in polysaccharides and release glucose and maltose.

SDS-PAGE is a biochemical technique used to separate charged molecules from a mixture, according to their molecular masses. SDS acts as a surfactant and charges the proteins both negatively and evenly. Thus, separating them only based on their molecular mass.

Western blot is a visualizing technique which is commonly used for analyzing proteins. Proteins separated by SDS-PAGE electrophoresis are transferred to a membrane, so that it can be visualized easily with the specific antibodies against the target protein.

Answer 6

Chapter 21, Problem 44P

Interpretation Introduction

(a)

Interpretation:

The reason for a “smear” in lane 1 of the western blot gel should be explained.

Concept introduction:

Glycogenin is an enzyme which catalyzes the formation of short glycogen primers from glucose molecules.

Alpha amylase catalyzes the hydrolysis of alpha linkages in polysaccharides and release glucose and maltose.

SDS-PAGE is a biochemical technique used to separate charged molecules from a mixture, according to their molecular masses. SDS acts as a surfactant and negatively charge the proteins evenly. Thus, separating them only based on their molecular mass.

Western blot is visualizing technique which is commonly used for analyzing proteins. Proteins separated by SDS-PAGE electrophoresis is transferred to a membrane, so that it can be visualized easily with the specific antibodies against the target protein.

Answer 7

Chapter 21, Problem 44P

Interpretation Introduction

(a)

Interpretation:

The reason for a “smear” in lane 1 of the western blot gel should be explained.

Concept introduction:

Glycogenin is an enzyme which catalyzes the formation of short glycogen primers from glucose molecules.

Alpha amylase catalyzes the hydrolysis of alpha linkages in polysaccharides and release glucose and maltose.

SDS-PAGE is a biochemical technique used to separate charged molecules from a mixture, according to their molecular masses. SDS acts as a surfactant and negatively charge the proteins evenly. Thus, separating them only based on their molecular mass.

Western blot is visualizing technique which is commonly used for analyzing proteins. Proteins separated by SDS-PAGE electrophoresis is transferred to a membrane, so that it can be visualized easily with the specific antibodies against the target protein.

Answer 8

Interpretation Introduction

(b)

Interpretation:

The significance of the decrease in high molecular weight bands in lane 2 should be explained.

Concept introduction:

Glycogenin is an enzyme which catalyzes the formation of short glycogen primers from glucose molecules.

Alpha amylase catalyzes the hydrolysis of alpha linkages in polysaccharides and release glucose and maltose.

SDS-PAGE is a biochemical technique used to separate charged molecules from a mixture, according to their molecular masses. SDS acts as a surfactant and negatively charge the proteins evenly. Thus, separating them only the basis of their molecular mass.

Western blot is a visualizing technique which is commonly used for analyzing proteins. Proteins separated by SDS-PAGE electrophoresis are transferred to a membrane, so that it can be visualized easily with the specific antibodies against the target protein.

Answer 9

Interpretation Introduction

(b)

Interpretation:

The significance of the decrease in high molecular weight bands in lane 2 should be explained.

Concept introduction:

Glycogenin is an enzyme which catalyzes the formation of short glycogen primers from glucose molecules.

Alpha amylase catalyzes the hydrolysis of alpha linkages in polysaccharides and release glucose and maltose.

SDS-PAGE is a biochemical technique used to separate charged molecules from a mixture, according to their molecular masses. SDS acts as a surfactant and negatively charge the proteins evenly. Thus, separating them only the basis of their molecular mass.

Western blot is a visualizing technique which is commonly used for analyzing proteins. Proteins separated by SDS-PAGE electrophoresis are transferred to a membrane, so that it can be visualized easily with the specific antibodies against the target protein.

Answer 10

Interpretation Introduction

(c)

Interpretation:

Significance of the difference between lane 2 and 3 should be explained

Concept introduction:

Glycogenin is an enzyme which catalyzes the formation of short glycogen primers from glucose molecules.

Alpha amylase catalyzes the hydrolysis of alpha linkages in polysaccharides and release glucose and maltose.

SDS-PAGE is a biochemical technique used to separate charged molecules from a mixture, according to their molecular masses. SDS acts as a surfactant and negatively charge the proteins evenly. Thus, separating them only based on their molecular mass.

Western blot is a visualizing technique which is commonly used for analyzing proteins. Proteins separated by SDS-PAGE electrophoresis is transferred to a membrane, so that it can be visualized easily with the specific antibodies against the target protein.

Answer 11

Interpretation Introduction

(c)

Interpretation:

Significance of the difference between lane 2 and 3 should be explained

Concept introduction:

Glycogenin is an enzyme which catalyzes the formation of short glycogen primers from glucose molecules.

Alpha amylase catalyzes the hydrolysis of alpha linkages in polysaccharides and release glucose and maltose.

SDS-PAGE is a biochemical technique used to separate charged molecules from a mixture, according to their molecular masses. SDS acts as a surfactant and negatively charge the proteins evenly. Thus, separating them only based on their molecular mass.

Western blot is a visualizing technique which is commonly used for analyzing proteins. Proteins separated by SDS-PAGE electrophoresis is transferred to a membrane, so that it can be visualized easily with the specific antibodies against the target protein.

Answer 12

Interpretation Introduction

(d)

Interpretation:

A plausible reason for no difference between lanes 3 and 4 should be suggested.

Concept introduction:

Glycogenin is an enzyme which catalyzes the formation of short glycogen primers from glucose molecules.

Alpha amylase catalyzes the hydrolysis of alpha linkages in polysaccharides and release glucose and maltose.

SDS-PAGE is a biochemical technique used to separate charged molecules from a mixture, according to their molecular masses. SDS acts as a surfactant and charges the proteins both negatively and evenly. Thus, separating them only based on their molecular mass.

Western blot is a visualizing technique which is commonly used for analyzing proteins. Proteins separated by SDS-PAGE electrophoresis are transferred to a membrane, so that it can be visualized easily with the specific antibodies against the target protein.

Answer 13

Interpretation Introduction

(d)

Interpretation:

A plausible reason for no difference between lanes 3 and 4 should be suggested.

Concept introduction:

Glycogenin is an enzyme which catalyzes the formation of short glycogen primers from glucose molecules.

Alpha amylase catalyzes the hydrolysis of alpha linkages in polysaccharides and release glucose and maltose.

SDS-PAGE is a biochemical technique used to separate charged molecules from a mixture, according to their molecular masses. SDS acts as a surfactant and charges the proteins both negatively and evenly. Thus, separating them only based on their molecular mass.

Western blot is a visualizing technique which is commonly used for analyzing proteins. Proteins separated by SDS-PAGE electrophoresis are transferred to a membrane, so that it can be visualized easily with the specific antibodies against the target protein.

Answer 14

Interpretation Introduction

(e)

Interpretation:

The 66 kDabands observed in all the lanes treated with amylase, despite the fact that the cells were treated differently should be justified.

Concept introduction:

Glycogenin is an enzyme which catalyzes the formation of short glycogen primers from glucose molecules.

Alpha amylase catalyzes the hydrolysis of alpha linkages in polysaccharides and release glucose and maltose.

SDS-PAGE is a biochemical technique used to separate charged molecules from a mixture, according to their molecular masses. SDS acts as a surfactant and charges the proteins both negatively and evenly. Thus, separating them only based on their molecular mass.

Western blot is a visualizing technique which is commonly used for analyzing proteins. Proteins separated by SDS-PAGE electrophoresis are transferred to a membrane, so that it can be visualized easily with the specific antibodies against the target protein.

Answer 15

Interpretation Introduction

(e)

Interpretation:

The 66 kDabands observed in all the lanes treated with amylase, despite the fact that the cells were treated differently should be justified.

Concept introduction:

Glycogenin is an enzyme which catalyzes the formation of short glycogen primers from glucose molecules.

Alpha amylase catalyzes the hydrolysis of alpha linkages in polysaccharides and release glucose and maltose.

SDS-PAGE is a biochemical technique used to separate charged molecules from a mixture, according to their molecular masses. SDS acts as a surfactant and charges the proteins both negatively and evenly. Thus, separating them only based on their molecular mass.

Western blot is a visualizing technique which is commonly used for analyzing proteins. Proteins separated by SDS-PAGE electrophoresis are transferred to a membrane, so that it can be visualized easily with the specific antibodies against the target protein.

Answer 16

Expert Solution & Answer

Answer 17

Expert Solution & Answer

Answer 18

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Answer 19

Ch. 21 - Prob. 1P Ch. 21 - Prob. 2P Ch. 21 - Prob. 3P Ch. 21 - Prob. 4P Ch. 21 - Prob. 5P Ch. 21 - Prob. 6P Ch. 21 - Prob. 7P Ch. 21 - Prob. 8P Ch. 21 - Prob. 9P Ch. 21 - Prob. 10P

Solution Summary: The author explains the reason for the "smear" in lane 1 of the western blot gel. Glycogenin catalyzes the formation of short glycogen primers from glucose molecules.

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Chapter 21 Solutions