Genetics: Analysis and Principles
6th Edition
ISBN: 9781259616020
Author: Robert J. Brooker Professor Dr.
Publisher: McGraw-Hill Education
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Chapter 21.6, Problem 2COMQ
The basis for DNase I footprinting is that the binding of a protein to DNA
a. prevents the DNA from being digested with a restriction enzyme.
b. enhances the ability of the DNA to be digested with a restriction enzyme.
c. prevents the DNA from being digested with DNase I.
d. enhances the ability of the DNA to be digested by DNase I.
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Students have asked these similar questions
A DNA fingerprint is based on _____.
a
repeating sequences found on noncoding regions
b
the presence of restriction enzymes
c
a single repeating region
d
repeating sequences found on coding regions
In Cohen-Boyer’s recombinant DNA procedure, ___i___ must be used for both the bacterial DNA and the amphibian DNA ___ii___
a) the same restriction enzyme; so that the restriction sites are identical in the DNA of each species
b) different restriction enzymes; So that the genes outside the restriction site are maintained
c) different restriction enzymes; to ensure that the newly introduced genes are maintained in the bacterial DNA
d) the same restriction enzyme; to ensure that the newly formed DNA can replicate
a) what are restriction enzymes?
b) What is the main function of restriction enzymes in nature?
c) Compare and contrast the these enzymes in nature and in scientific research.
Chapter 21 Solutions
Genetics: Analysis and Principles
Ch. 21.1 - 1. Which of the following may be used as a vector...Ch. 21.1 - The restriction enzymes used in gene-cloning...Ch. 21.1 - 3. Which is the proper order of the following...Ch. 21.1 - 4. The function of reverse transcriptase is...Ch. 21.1 - A collection of recombinant vectors that carry...Ch. 21.2 - Prob. 1COMQCh. 21.2 - Prob. 2COMQCh. 21.2 - 3. During real-time PCR, the synthesis of PCR...Ch. 21.3 - When a dideoxyribonucleotide is incorporated into...Ch. 21.4 - 1. The purpose of site-directed mutagenesis and...
Ch. 21.5 - Which of the following methods use(s) a labeled...Ch. 21.5 - 2. Which of the following methods is used to...Ch. 21.5 - During Western blotting, the primary antibody...Ch. 21.6 - 1. In an EMSA, the binding of a protein to...Ch. 21.6 - The basis for DNase I footprinting is that the...Ch. 21 - Discuss three important advances that have...Ch. 21 - Prob. 2CONQCh. 21 - Write a double-stranded DNA sequence that is 20...Ch. 21 - What is cDNA? In eukaryotes, how does cDNA differ...Ch. 21 - 5. Draw the structural feature of a...Ch. 21 - Prob. 1EQCh. 21 - Prob. 2EQCh. 21 - Describe the important features of cloning...Ch. 21 - 4. How does gene cloning produce many copies of a...Ch. 21 - Prob. 5EQCh. 21 - Prob. 6EQCh. 21 - Prob. 7EQCh. 21 - Prob. 8EQCh. 21 - Prob. 9EQCh. 21 - Starting with a sample of RNA that contains the...Ch. 21 - 11. What type of probe is used for real-time PCR?...Ch. 21 - 12. What phase of PCR (exponential, linear, or...Ch. 21 - 13. DNA sequencing can help us to identify...Ch. 21 - A sample of DNA was subjected to automated DNA...Ch. 21 - Prob. 15EQCh. 21 - Prob. 16EQCh. 21 - Prob. 17EQCh. 21 - Prob. 18EQCh. 21 - Prob. 19EQCh. 21 - What is the purpose of a Northern blotting...Ch. 21 - Prob. 21EQCh. 21 - Prob. 22EQCh. 21 - 23. In the Western blot shown here, proteins were...Ch. 21 - If you wanted to know if a protein was made during...Ch. 21 - Prob. 25EQCh. 21 - Prob. 26EQCh. 21 - Prob. 27EQCh. 21 - 28. Describe the rationale behind the...Ch. 21 - Certain hormones, such as epinephrine, can...Ch. 21 - An electrophoretic mobility shift assay can be...Ch. 21 - Prob. 31EQCh. 21 - Prob. 32EQCh. 21 - Prob. 33EQCh. 21 - Prob. 1QSDC
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Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.Similar questions
- Put the following tasks in the order they would occur during a DNA cloning experiment. a. using DNA ligase to seal DNA fragments into vectors b. using a probe to identify a clone in the library c. sequencing the DNA of the clone d. making a DNA library of clones e. cutting genomic DNA with restriction enzymesarrow_forwarda. Using nucleotide letters, show the kind of cut that could be made on a DNA molecule to circularize it into a plasmid. b. What are restriction length polymorphisms, and how are they used?arrow_forwardFrom where do we get primers for sequencing DNA? A) they are synthesized by reverse transcriptase B) they are cut out of plasmids using restriction endonucleases C) DNA primase is added to the sequencing reaction and synthesizes the primers D) biotechnology companies synthesize them using organic chemistryarrow_forward
- A. A plasmid is shown with the locations of various restriction enzyme sites labeled. If you cut the plasmid with Xhol and Xbal, which lane of the agarose gel represents the DNA fragments you would expect from the digestion? B. If you now decide to cut the plasmid with EcoRI, how many fragments will be produced and what will their sizes be? C. When running DNA samples on agarose gel, an electric field is applied. Towards which electrode will the DNA migrate and why?arrow_forwardWhat are the sticky ends of the restriction fragments? Select one: a. The surfaces of sticky ends contain matching base pairs, allowing fragments to splice. b. The surfaces of sticky ends have glue like substance that allow fragments to splice. c. The surfaces of sticky ends contain the exact same nucleotides, allowing fragments to bond. d. The surfaces of sticky ends have velcro like structure, allowing fragments to bond.arrow_forwardRestriction enzymes (type II) bind to their recognition site and A. begin elongation from it B. methylaet it C. cut inside it D. ligate itarrow_forward
- If you knew the sequence of a gene in one organism, how could you determine if another organism had a similar gene? A. insert the known gene into a vector and use the vector to insert the known gene into the other organism B. treat the genomes of both organisms with the same restriction enzyme and compare the patterns of the bands produced with gel electrophoresis C. create a hybrid of the two organisms by breeding them and check for mutations D. create labeled DNA probes from the known gene and use them to search the genome of the other organismarrow_forwardYou are trying to clone a gene, You have successfully isolated it from the genomic DNA of an organism using the Hindill restriction enzyme. You then take a plasmid with a single EcoRI restriction site and cleave it with EcoRI. You combine these two fragments and treat them with DNA ligase. Answer the two questions below. a. Does the cloning reaction succeed as described? If so, what is the product obtained? b. Explain your answer above,arrow_forwardDescribe What are the sticky ends of the restriction fragments? Select one: a. The surfaces of sticky ends contain matching base pairs, allowing fragments to splice. b. The surfaces of sticky ends have glue like substance that allow fragments to splice. c. The surfaces of sticky ends contain the exact same nucleotides, allowing fragments to bond. d. The surfaces of sticky ends have velcro like structure, allowing fragments to bond.arrow_forward
- The restriction endonucleases used in recombinant DNA work: a. are synthesized by bacteria b. recognize sequences 14-16 bp long c. cut the DNA outside the recognition sequence d. all the above are truearrow_forwarda. Why do bacteria make restriction endonucleases? b. What is it about the endonucleases that prevents bacteria from destroying their own DNA?arrow_forwardwhich of the following do researchers not need to use during vector cloning? a. a plasmid containing selectable marker genes such as beta galactosidase or ampicillin resistance genes. b. restriction enzymes. c. DNA polymerase d. a growth medium with carefully selected ingredients that take advantage of selectable markers. e. none of the above.arrow_forward
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