Genetics: Analysis and Principles
6th Edition
ISBN: 9781259616020
Author: Robert J. Brooker Professor Dr.
Publisher: McGraw-Hill Education
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Textbook Question
Chapter 21, Problem 20EQ
What is the purpose of a Northern blotting experiment? What types of information can it tell you about the transcription of a gene?
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Chapter 21 Solutions
Genetics: Analysis and Principles
Ch. 21.1 - 1. Which of the following may be used as a vector...Ch. 21.1 - The restriction enzymes used in gene-cloning...Ch. 21.1 - 3. Which is the proper order of the following...Ch. 21.1 - 4. The function of reverse transcriptase is...Ch. 21.1 - A collection of recombinant vectors that carry...Ch. 21.2 - Prob. 1COMQCh. 21.2 - Prob. 2COMQCh. 21.2 - 3. During real-time PCR, the synthesis of PCR...Ch. 21.3 - When a dideoxyribonucleotide is incorporated into...Ch. 21.4 - 1. The purpose of site-directed mutagenesis and...
Ch. 21.5 - Which of the following methods use(s) a labeled...Ch. 21.5 - 2. Which of the following methods is used to...Ch. 21.5 - During Western blotting, the primary antibody...Ch. 21.6 - 1. In an EMSA, the binding of a protein to...Ch. 21.6 - The basis for DNase I footprinting is that the...Ch. 21 - Discuss three important advances that have...Ch. 21 - Prob. 2CONQCh. 21 - Write a double-stranded DNA sequence that is 20...Ch. 21 - What is cDNA? In eukaryotes, how does cDNA differ...Ch. 21 - 5. Draw the structural feature of a...Ch. 21 - Prob. 1EQCh. 21 - Prob. 2EQCh. 21 - Describe the important features of cloning...Ch. 21 - 4. How does gene cloning produce many copies of a...Ch. 21 - Prob. 5EQCh. 21 - Prob. 6EQCh. 21 - Prob. 7EQCh. 21 - Prob. 8EQCh. 21 - Prob. 9EQCh. 21 - Starting with a sample of RNA that contains the...Ch. 21 - 11. What type of probe is used for real-time PCR?...Ch. 21 - 12. What phase of PCR (exponential, linear, or...Ch. 21 - 13. DNA sequencing can help us to identify...Ch. 21 - A sample of DNA was subjected to automated DNA...Ch. 21 - Prob. 15EQCh. 21 - Prob. 16EQCh. 21 - Prob. 17EQCh. 21 - Prob. 18EQCh. 21 - Prob. 19EQCh. 21 - What is the purpose of a Northern blotting...Ch. 21 - Prob. 21EQCh. 21 - Prob. 22EQCh. 21 - 23. In the Western blot shown here, proteins were...Ch. 21 - If you wanted to know if a protein was made during...Ch. 21 - Prob. 25EQCh. 21 - Prob. 26EQCh. 21 - Prob. 27EQCh. 21 - 28. Describe the rationale behind the...Ch. 21 - Certain hormones, such as epinephrine, can...Ch. 21 - An electrophoretic mobility shift assay can be...Ch. 21 - Prob. 31EQCh. 21 - Prob. 32EQCh. 21 - Prob. 33EQCh. 21 - Prob. 1QSDC
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- What factors do we consider in genetic transcription: precision, accuracy, or a combination of the two? What happens to the genetic coding if it encounters an error during the process of forming. Cite a few instances where coding was thrown off track.arrow_forward“How will raising the temperature affect the transcription regulation of RodA? and what is the importance of performing this lab?arrow_forwardhow to design an experiment to test weather the mutation is on enhancer, promoter, or protein-coding region?arrow_forward
- Different forms of a protein are produced in the liver and in the brain by alternate splicing of its mRNA. How would a researcher clone the gene encoding that protein to study the form that was produced in the liver?arrow_forwardHow can deletion experiments within gene sequences help identify important DNA sequences for the expression of a gene? Discuss how this was done to initially identify UP elements. What is the result of deleting an UP element?arrow_forwardHow geneticists used synthetic mRNAs to limit the coding possibilities?arrow_forward
- What do transposable genes mean? How many different transposable genes do bacteria contain?arrow_forwardWhat is lac operon? Draw and/or identify the status of the lac operon in a given set of environmental conditions and/or cell mutations.arrow_forwardWhat is the role of the sgRNA in genome editing?arrow_forward
- What is a promoter? Is it located at the upstream ordownstream end of a transcription unit?arrow_forwardWhat would be the consequence of inserting 10 random base pairs immediately downstream of the -10 box in a strong promoter? And What sort of lab techniques/experiments could be used to determine these consequences?arrow_forwardBesides the great potential of gene editing, what are the biggest risks?  How do scientist test to ensure their gene editing experiments target only the desired specific genes?arrow_forward
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