Genetics: Analysis and Principles
6th Edition
ISBN: 9781259616020
Author: Robert J. Brooker Professor Dr.
Publisher: McGraw-Hill Education
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Chapter 21.1, Problem 3COMQ
Which is the proper order of the following steps in a gene-cloning experiment involving vectors?
1. Add DNA ligase.
2. Incubate the chromosomal DNA and the vector DNA with a restriction enzyme.
3. Introduce the DNA into living cells.
4. Mix the chromosomal DNA and vector DNA together.
a. 1, 2, 3, 4 c. 2, 4, 1, 3
b. 2, 3, 1, 4 d. 1, 2, 4, 3
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Regarding the PCR technique, what is false?a. It can produce multiple copies of DNA.b. It is the same as DNA fingerprinting.c. It is not a time-consuming process.d. It cannot successfully copy whole genes
Explain briefly within 5 sentences each
1. a. What is PCR? Explain/relate its importance in DNA marker analysis.
1.b. What are restriction enzymes (RE)? Describe how a RE can be used to develop/design a DNAmarker.
a) what are restriction enzymes?
b) What is the main function of restriction enzymes in nature?
c) Compare and contrast the these enzymes in nature and in scientific research.
Chapter 21 Solutions
Genetics: Analysis and Principles
Ch. 21.1 - 1. Which of the following may be used as a vector...Ch. 21.1 - The restriction enzymes used in gene-cloning...Ch. 21.1 - 3. Which is the proper order of the following...Ch. 21.1 - 4. The function of reverse transcriptase is...Ch. 21.1 - A collection of recombinant vectors that carry...Ch. 21.2 - Prob. 1COMQCh. 21.2 - Prob. 2COMQCh. 21.2 - 3. During real-time PCR, the synthesis of PCR...Ch. 21.3 - When a dideoxyribonucleotide is incorporated into...Ch. 21.4 - 1. The purpose of site-directed mutagenesis and...
Ch. 21.5 - Which of the following methods use(s) a labeled...Ch. 21.5 - 2. Which of the following methods is used to...Ch. 21.5 - During Western blotting, the primary antibody...Ch. 21.6 - 1. In an EMSA, the binding of a protein to...Ch. 21.6 - The basis for DNase I footprinting is that the...Ch. 21 - Discuss three important advances that have...Ch. 21 - Prob. 2CONQCh. 21 - Write a double-stranded DNA sequence that is 20...Ch. 21 - What is cDNA? In eukaryotes, how does cDNA differ...Ch. 21 - 5. Draw the structural feature of a...Ch. 21 - Prob. 1EQCh. 21 - Prob. 2EQCh. 21 - Describe the important features of cloning...Ch. 21 - 4. How does gene cloning produce many copies of a...Ch. 21 - Prob. 5EQCh. 21 - Prob. 6EQCh. 21 - Prob. 7EQCh. 21 - Prob. 8EQCh. 21 - Prob. 9EQCh. 21 - Starting with a sample of RNA that contains the...Ch. 21 - 11. What type of probe is used for real-time PCR?...Ch. 21 - 12. What phase of PCR (exponential, linear, or...Ch. 21 - 13. DNA sequencing can help us to identify...Ch. 21 - A sample of DNA was subjected to automated DNA...Ch. 21 - Prob. 15EQCh. 21 - Prob. 16EQCh. 21 - Prob. 17EQCh. 21 - Prob. 18EQCh. 21 - Prob. 19EQCh. 21 - What is the purpose of a Northern blotting...Ch. 21 - Prob. 21EQCh. 21 - Prob. 22EQCh. 21 - 23. In the Western blot shown here, proteins were...Ch. 21 - If you wanted to know if a protein was made during...Ch. 21 - Prob. 25EQCh. 21 - Prob. 26EQCh. 21 - Prob. 27EQCh. 21 - 28. Describe the rationale behind the...Ch. 21 - Certain hormones, such as epinephrine, can...Ch. 21 - An electrophoretic mobility shift assay can be...Ch. 21 - Prob. 31EQCh. 21 - Prob. 32EQCh. 21 - Prob. 33EQCh. 21 - Prob. 1QSDC
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- A) What are the three main steps involved in PCR? Include temperatures and descriptions of each step. B) Explain why RFLP can produce many bands on an electrophoresis gel and PCR (one set of primers) will only produce one or two bands on a gel for the same genome.arrow_forwardChoose the one answer that fits best. Which statement regarding Molecular Biology is NOT correct (videos)? a. Taq Polymerase was isolated from an organism found in Yellowstone Park b. Restriction enzymes leave sticky ends c. DNA sequencing allows us to read DNA sequences d. Restriction enzymes cut DNA at specific sites e. EcoRI and HindII are commonly used polymerasesarrow_forwardWhich is the proper order of the following steps in a genecloningexperiment involving vectors?1. Add DNA ligase.2. Incubate the chromosomal DNA and the vector DNA with arestriction enzyme.3. Introduce the DNA into living cells.4. Mix the chromosomal DNA and vector DNA together.a. 1, 2, 3, 4 c. 2, 4, 1, 3b. 2, 3, 1, 4 d. 1, 2, 4, 3arrow_forward
- If you knew the sequence of a gene in one organism, how could you determine if another organism had a similar gene? A. insert the known gene into a vector and use the vector to insert the known gene into the other organism B. treat the genomes of both organisms with the same restriction enzyme and compare the patterns of the bands produced with gel electrophoresis C. create a hybrid of the two organisms by breeding them and check for mutations D. create labeled DNA probes from the known gene and use them to search the genome of the other organismarrow_forwardWhat is the purpose of the low temperature step in the PCR reaction? a. To allow DNA polymerase to synthesize new DNA in the 3' to 5' direction b. To permanently deactivate DNA polymerase c. To allow primers to anneal to DNA templates d. To allow DNA polymerase to synthesize new DNA in the 5' to 3' directionarrow_forwardIn the reproductive cloning of an animal, the genome of the cloned individual comes from ________ a. a sperm cell b. an egg cell c. any gamete cell d. a body cellarrow_forward
- Which technique allows a researcher to change a single specific nucleotide in a gene sequence in vitro? a. The CRISPR-Cas9 system b. PCR-based random mutagenesis c. PCR-based site-specific mutagenesis d. RT-PCRarrow_forwardWhen constructing a recombinant DNA molecule, a marker gene is used to: a. give the organism a new trait, such as insect resistance b. Identify whether the transformed organism contains the recombinant DNA c. replicate (copy) the gene of interest d. Introduce the recombinant DNA into an organism e. cut short sequences of DNAarrow_forwardWhat is a cloning vector? A. The DNA probe used to locate a particular gene in the genome. B. An agent such as plasmid, used to transfer DNA from an in vitro solution into a living cell. C. The laboratory apparatus used to clone genes. D. An enzyme that cuts DNA into restriction fragments.arrow_forward
- Choose the phrase that best describes the role of a cloning vector. A) Separates fragments of DNA B) Detects the presence of specific DNA sample C) Isolates DNA from a donor organism D) Screens for recombinants that have been successfully transformed E) Introduces DNA into a cloning hostarrow_forwardPut the following tasks in the order they would occur during a DNA cloning experiment. a. using DNA ligase to seal DNA fragments into vectors b. using a probe to identify a clone in the library c. sequencing the DNA of the clone d. making a DNA library of clones e. cutting genomic DNA with restriction enzymesarrow_forwardWould it be possible to use human polymerase for the PCR reaction? a. No, because human polymerase does not have the ability to withstand the high temperatures required for the PCR reaction to occur. b. No, because human polymerase cannot be extracted from cells to use in a lab setting. c. Yes, because we are using human DNA as the template DNA. d. Yes, because human polymerase can add bases to a template strand without a primer.arrow_forward
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