Genetics: Analysis and Principles
6th Edition
ISBN: 9781259616020
Author: Robert J. Brooker Professor Dr.
Publisher: McGraw-Hill Education
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Chapter 21.2, Problem 1COMQ
Summary Introduction
Introduction:
The PCR (polymerase chain reaction) is the technique that is used to producemany copies of the specific DNA (deoxyribonucleic acid). This technique is based on the use of DNA polymerase enzyme.
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Describe the three steps of a PCR cycle.
In one PCR cycle, the correct order of steps isa. primer annealing, primer extension, denaturation.b. primer annealing, denaturation, primer extension.c. denaturation, primer annealing, primer extension.d. denaturation, primer extension, primer annealing.
You have been performing a PCR reaction but your results aren't the greatest. Your Supervisor has told you that you should increase the concentration of Magnesium. What affect will this have on the reaction?
a.
The annealing temperature will not be affected but the enzyme activity will be affected.
b.
The annealing temperature will decrease.
c.
The denaturation temparture will have to be increased in the PCR protocol.
d.
The denaturation temparture will have to be decreased in the PCR protocol.
e.
The Annealing temperature will increase.
Chapter 21 Solutions
Genetics: Analysis and Principles
Ch. 21.1 - 1. Which of the following may be used as a vector...Ch. 21.1 - The restriction enzymes used in gene-cloning...Ch. 21.1 - 3. Which is the proper order of the following...Ch. 21.1 - 4. The function of reverse transcriptase is...Ch. 21.1 - A collection of recombinant vectors that carry...Ch. 21.2 - Prob. 1COMQCh. 21.2 - Prob. 2COMQCh. 21.2 - 3. During real-time PCR, the synthesis of PCR...Ch. 21.3 - When a dideoxyribonucleotide is incorporated into...Ch. 21.4 - 1. The purpose of site-directed mutagenesis and...
Ch. 21.5 - Which of the following methods use(s) a labeled...Ch. 21.5 - 2. Which of the following methods is used to...Ch. 21.5 - During Western blotting, the primary antibody...Ch. 21.6 - 1. In an EMSA, the binding of a protein to...Ch. 21.6 - The basis for DNase I footprinting is that the...Ch. 21 - Discuss three important advances that have...Ch. 21 - Prob. 2CONQCh. 21 - Write a double-stranded DNA sequence that is 20...Ch. 21 - What is cDNA? In eukaryotes, how does cDNA differ...Ch. 21 - 5. Draw the structural feature of a...Ch. 21 - Prob. 1EQCh. 21 - Prob. 2EQCh. 21 - Describe the important features of cloning...Ch. 21 - 4. How does gene cloning produce many copies of a...Ch. 21 - Prob. 5EQCh. 21 - Prob. 6EQCh. 21 - Prob. 7EQCh. 21 - Prob. 8EQCh. 21 - Prob. 9EQCh. 21 - Starting with a sample of RNA that contains the...Ch. 21 - 11. What type of probe is used for real-time PCR?...Ch. 21 - 12. What phase of PCR (exponential, linear, or...Ch. 21 - 13. DNA sequencing can help us to identify...Ch. 21 - A sample of DNA was subjected to automated DNA...Ch. 21 - Prob. 15EQCh. 21 - Prob. 16EQCh. 21 - Prob. 17EQCh. 21 - Prob. 18EQCh. 21 - Prob. 19EQCh. 21 - What is the purpose of a Northern blotting...Ch. 21 - Prob. 21EQCh. 21 - Prob. 22EQCh. 21 - 23. In the Western blot shown here, proteins were...Ch. 21 - If you wanted to know if a protein was made during...Ch. 21 - Prob. 25EQCh. 21 - Prob. 26EQCh. 21 - Prob. 27EQCh. 21 - 28. Describe the rationale behind the...Ch. 21 - Certain hormones, such as epinephrine, can...Ch. 21 - An electrophoretic mobility shift assay can be...Ch. 21 - Prob. 31EQCh. 21 - Prob. 32EQCh. 21 - Prob. 33EQCh. 21 - Prob. 1QSDC
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- In a typical PCR reaction, describe what is happening in stages occurring at temperature ranges (a) 92–95°C, (b) 45–65°C, and (c) 65–75°C.arrow_forwardWhich of the following reagents is NOT required for a PCR? a. DNA template b. DNA Polymerase c. All four dNTPs d. small amount of ddNTPs e. Pair of primersarrow_forwardList the steps of a PCR cyclearrow_forward
- Please answer these two questions regarding PCR: a) Why do you need to perform PCR on DNA obtained from a crime scene? b) Why so forensic labs analyze non-coding DNA rather than genes?arrow_forwardIn sequential order, what are the three steps of PCR? A. Denature DNA, Anneal Primers, Extend DNA B. Denature DNA, Extend DNA, Anneal Primers C. Anneal Primers, Extend DNA, Denature DNA D. Extend DNA, Anneal Primers, Denature DNAarrow_forwardAll are true about PCR except A. Automated PCR machine called a thermal cycler B. A thermostable DNA polymerase is required C. Primer extension occurs at 72˚C D. Annealing involves binding of primer between 90-98˚Carrow_forward
- The three steps involved in PCR are: Question 4 options: Denaturation, primer annealing, and extension Denaturation, ligation, and extension Denaturation, template annealing, and extension Denaturation, primer annealing, and exhaustionarrow_forwardDuring DNA isolation, you incubated the tissue sample at 55°C for 60 minutes, followed by incubation at 95°C for 10 minutes. What happens to the DNA molecules in this last 95°C incubation? What would happen to the PCR if this step was omitted?arrow_forwardWhat is the improtance of creating the master mix in the PCR lab? Pick all that apply. a. It makes the reaction run faster. b. It ensures more consistent concentrations of reagents for each tube. c. It helps correct for pipette errors at small volumes. d. It is easier to set up a single tube to add needed components in bulk versus pippetting each sample tube individually.arrow_forward
- All of the following are needed for PCR except: A) dNTP nucleotide monomers B) Oligonucleotide primers C) Restriction endonuclease D) Template DNA E) DNA polymerasearrow_forwardRegarding the PCR technique, what is false?a. It can produce multiple copies of DNA.b. It is the same as DNA fingerprinting.c. It is not a time-consuming process.d. It cannot successfully copy whole genesarrow_forwardCite three examples of an application of PCR (polymerase chain reaction).arrow_forward
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