Biochemistry
9th Edition
ISBN: 9781319114671
Author: Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher: W. H. Freeman
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Chapter 31, Problem 33P
Interpretation Introduction
Interpretation:
The mechanism by which the ribosome identifies the AUG start codon, should be determined.
Concept introduction:
Protein synthesis (translation) is a process of generating new protein sequences inside the cell. This process takes place in the cytoplasm of the cell. This process is balanced by the degradation or export of cellular proteins. It is constituted of three steps namely, initiation, elongation, and termination.
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DNA A G T A C C G G G C A A A C T G C A T T G T G
mRNA U C A U G G C C C G U U U G A C G U A A C A C
Use the "Genetic Code Chart" to determine the sequence of amino acids in your polypeptide chain. Remember to START translation at the start codon by adding a Methionine and STOP translating when you reach a stop codon.
UCAAUGGGGUUUAUAGCG… (there are bases after the last G but we don’t know what they are so there may be some ambiguity for the last codon with regards to the amino acid possibilities)
if the translation frame had UCA as the first codon:
if the translation frame had CAA as the first codon:
if the translation frame had AAT as the first codon:
13c give the codon sequences of every code on this tRNA with the anti-codon 5AAG3, could pair with including wobble matches
Chapter 31 Solutions
Biochemistry
Ch. 31 - Prob. 1PCh. 31 - Prob. 2PCh. 31 - Prob. 3PCh. 31 - Prob. 4PCh. 31 - Prob. 5PCh. 31 - Prob. 6PCh. 31 - Prob. 7PCh. 31 - Prob. 8PCh. 31 - Prob. 9PCh. 31 - Prob. 10P
Ch. 31 - Prob. 11PCh. 31 - Prob. 12PCh. 31 - Prob. 13PCh. 31 - Prob. 14PCh. 31 - Prob. 15PCh. 31 - Prob. 16PCh. 31 - Prob. 17PCh. 31 - Prob. 18PCh. 31 - Prob. 19PCh. 31 - Prob. 20PCh. 31 - Prob. 21PCh. 31 - Prob. 22PCh. 31 - Prob. 23PCh. 31 - Prob. 24PCh. 31 - Prob. 25PCh. 31 - Prob. 26PCh. 31 - Prob. 27PCh. 31 - Prob. 28PCh. 31 - Prob. 29PCh. 31 - Prob. 30PCh. 31 - Prob. 31PCh. 31 - Prob. 32PCh. 31 - Prob. 33PCh. 31 - Prob. 34PCh. 31 - Prob. 35PCh. 31 - Prob. 36PCh. 31 - Prob. 37PCh. 31 - Prob. 38PCh. 31 - Prob. 39PCh. 31 - Prob. 40PCh. 31 - Prob. 41PCh. 31 - Prob. 42PCh. 31 - Prob. 43PCh. 31 - Prob. 44PCh. 31 - Prob. 45PCh. 31 - Prob. 46PCh. 31 - Prob. 47PCh. 31 - Prob. 48PCh. 31 - Prob. 49PCh. 31 - Prob. 50PCh. 31 - Prob. 51PCh. 31 - Prob. 52P
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- Need help Below is a strand of mRNA with three codons listed on the strand. Imagine the mRNA strand is in the cytoplasm of a cell and translation is in progress. Draw and label all the necessary main players needed for translation to occur. Included in your drawing should also bee 3 tRNAs , these 3 tRNAs should represent two different forms of tRNA. MRNA 5' ------------AUG------------AGG----------GAGarrow_forwardPLEASE ANSWER WHY? Some substitution mutation result in a malfunctioning protein but others do not. Why is this? arrow_forward1e) Give the sequence of every codon this tRNA, with the anticodon 5'AGG3', could base pair with (perfect and wobble matches), and name the amino acid coded for by each codon whose sequence you have written.arrow_forward
- Which choice best fits the blank? Refer to picture. The ribosome moves along the mRNA strand. In panels b, c, and d, new tRNAs carrying ___________. match up with the codons of the mRNA strand. After each tRNA locks into place, a peptide bond forms between the amino acid the tRNA is carrying and the amino acid already there. This process repeats until the end of the sequence is reached. A. ProteinsB. NucleotidesC. Amino Acidsarrow_forwardBacteria or Eukaryotes? Formation of a termination loop within the transcript Alternative splicing of transcripts Translation beginning before transcription is complete Cleavage following the AAUAAA signal Direct binding of RNA polymerase to promoterarrow_forwardRNA polymerase from E. coli (core enzyme alone) has all of the following properties except: a)requires all four ribonucleoside triphosphates and a DNA template. b)can extend an RNA chain and initiate a new chain. c)recognizes specific start signals in DNA. d)produces an RNA polymer that begins with a 5'-triphosphate. e)is required for the synthesis of mRNA, rRNA, and tRNA in E. coli.arrow_forward
- The images shown depict the initiation and elongation steps in protein translation. Arrange the images in the order in which these steps occur. A. B A C D B. B D A C C. C D A B D. A B C Darrow_forwardPlease help me answer the GENETIC CODE problems 1. Describe the expirement that allowed researchers to first identify the codon for a specific amino acid. 2. Which of the following statemts about the genetic code are true and which are false? Please correct each false statement. a. Each codon is composed of four bases b. Some amino acids are represented by more than one codon c.All codon represent an amino acid d. Each living species is thought to have its own unique genetic code. e. The codon AUG at the beginning of a sequence is a signal for protein synthesis to begin at that codon. f. It is not known if the code contains stop signals for protein synthesis. Thank you very much for your help.arrow_forwardCodons The genetic code consists of triplets of nucleotides called codons. Refer to the genetic code chart provided to answer the following questions. What is unique about the codon AUG? 2. What is unique about the codon UGA?arrow_forward
- What polypeptides would be formed from the sequence UCAATGGGGUUUAUAGCG… (there are bases after the last G but we don’t know what they are so there may be some ambiguity for the last codon with regards to the amino acid possibilities) if the translation frame had UCA as the first codon: if the translation frame had CAA as the first codon: if the translation frame had AAT as the first codon:arrow_forward2a) In prokaryotes, a small ribosomal subunit can potentially get on an mRNA anywhere it can find enough space to do so. Once a small ribosomal subunit has bound to an mRNA, it will scan along that mRNA in the 5' to 3' direction looking for a start codon at which to initiate translation. How does the small ribosomal subunit distinguish a start codon from any other AUG codon that simply codes for methionine in the middle of a coding sequence?arrow_forwardThe template strand (notice the directionality) of DNA that is known to encode the N-terminal region of an E. coliprotein has the following nucleotide sequence:5′–GTAGCGTTCCATCAGATTT–3′Give the sequence of the first four amino acids of the protein (label the N-terminus).arrow_forward
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