(a)
Interpretation:
Effects on eIF-4 helicase activity in the presence of eIF-4H should be determined.
Concept introduction:
Eukaryotic initiation factors (eIFs) are the proteins, which are used during the initiation of the eukaryotic translation process. The function of these proteins is to stabilize the ribosomal complexes that are near the start codon on the mRNA strand.
(b)
Interpretation:
The importance of measuring the helicase activity of eIF-4H as control should be determined.
Concept introduction:
Eukaryotic initiation factors (eIFs) are the proteins, which are used during the initiation of the eukaryotic translation process. The function of these proteins is to stabilize the ribosomal complexes that are near the start codon on the mRNA strand.
(c)
Interpretation:
The ratio of eIF-4H and eIF-4 should be determined to attain optimal activity.
Concept introduction:
Eukaryotic initiation factors (eIFs) are the proteins, which are used during the initiation of eukaryotic translation process. The function of these proteins is to stabilize the ribosomal complexes that are near the start codon on the mRNA strand.
(d)
Interpretation:
The varying effect f eIF-4H with helix stability should be determined.
Concept introduction:
Eukaryotic initiation factors (eIFs) are the proteins, which are used during the initiation of the eukaryotic translation process. The function of these proteins is to stabilize the ribosomal complexes that are near the start codon on the mRNA strand.
(e)
Interpretation:
The effect of eIF-4H on the helicase activity of eIF-4A should be determined.
Concept introduction:
Eukaryotic initiation factors (eIFs) are the proteins, which are used during the initiation of eukaryotic translation process. The function of these proteins is to stabilize the ribosomal complexes that are near the start codon on the mRNA strand.
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