Genetic Analysis: An Integrated Approach (3rd Edition)
Genetic Analysis: An Integrated Approach (3rd Edition)
3rd Edition
ISBN: 9780134605173
Author: Mark F. Sanders, John L. Bowman
Publisher: PEARSON
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Chapter 8, Problem 23P
Summary Introduction

To review:

The processes of transcription and translation as “coupled” in bacteria is described by Microbiologists. This term indicates that a bacterial mRNA can be undergoing transcription, when it is undergoing translation.

a) The coupling of possible transcription and translation in bacteria is to be described.

b) The possibility of coupling of transcription and translation in single-celled eukaryotes such as yeast is to be cheked.

Introduction:

Transcription is the process of synthesis of new RNA strands from the template one. There is a synthesis of an RNA strand from deoxy nucleotides of the sense strand. The formation of mRNA replaces the Thymine nucleotide with Uracil. RNA synthesis occurs in the direction of 5'-3' end. Any one of the DNA strands is selected for transcription. In bacteria, to save energy, the process is coupled –transcription and translation, while in eukaryotes, the process is divided and separate - transcription occurs in the nucleus and translation in the cytoplasm. In prokaryotes- the process is DNA-RNA-proteins, whereas in Eukaryotes- DNA-mRNA- RNA processing (posttranscriptional modification) - proteins.

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Shown below is a double-stranded bacterial (E. coli) DNA sequence coding for a hypothetical protein. The nucleotides are numbered 1 to 100.  a)Although the transcription start site begins at the underlined C/G, which of the following is the nucleotide sequences needed upstream for transcription to actually occur? b)What are the first 15 nucleotides of the mRNA? c)What are the first 5 amino acids translated from the resulting mRNA? d)A different mutation results in the substitution of the T/A base pair at position 30 (shown in bold and underlined) with a G/C base pair. How would this mutation affect the sequence of the protein that is produced?
Microbiologists describe the processes of transcription and translation as “coupled” in bacteria. This term indicates that bacterial mRNA can be undergoing transcription at the same moment it is also undergoing translation. How is coupling possible in bacteria? Is coupling of transcription and translation possible in single-celled eukaryotes, such as yeast? Why or why not?
Given the template DNA sequence:  3’ - TAC - CAG - GTT - ACC - ATC - 5’    A.) What will be the mRNA requence corresponding to the template DNA sequence? B.) What is the amino acid sequence in letter A? ( e.g. Arg, Phe, etc.)  C.) If the coding sequence of the dsDNA will "serve" as the template for transcription, what is the corresponding mRNA sequence? D.) With the mRNA transcript in letter C, what will be the amino acid sequence?  ( e.g. Arg, Phe, etc.)

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Genetic Analysis: An Integrated Approach (3rd Edition)

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