Microbiology: An Introduction
12th Edition
ISBN: 9780321929150
Author: Gerard J. Tortora, Berdell R. Funke, Christine L. Case
Publisher: PEARSON
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Textbook Question
Chapter 8, Problem 7MCQ
Mechanism by which the presence of glucose inhibits the lac operon.
Use the following choices to answer questions 7 and 8.
- a. catabolite repression
- b. DNA polymerase
- c. induction
- d. repression
- e. translation
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Gene expression in bacteria is controlled by the operon model.
a) Why is the operon model important?
b) Why is the trp operon known as a repressible operon?
What would occur if the repressor of an inducible operon weremutated so it could not bind the operator?(A) irreversible binding of the repressor to the promoter(B) reduced transcription of the operon’s genes(C) buildup of a substrate for the pathway controlled by theoperon(D) continuous transcription of the operon’s genes
If β-galactosidase is expressed when lactose is absent, what does this suggest about the lac operon?
a)
There is a mutation in the repressor protein
b)
There is a mutation in the catabolite activator protein
c)
There is a mutation in the promoter region
d)
There is a mutation in the luxI protein
Chapter 8 Solutions
Microbiology: An Introduction
Ch. 8 - Briefly describe the components of DNA, and...Ch. 8 - DRAW IT Identify and mark each of the following on...Ch. 8 - Match the following examples of mutagens. Column A...Ch. 8 - The following is a code for a strand of DNA. a....Ch. 8 - Identify when (before transcription, after...Ch. 8 - Which sequence is the best target for damage by UV...Ch. 8 - You are provided with cultures with the following...Ch. 8 - Why are mutation and recombination important in...Ch. 8 - NAME IT Normally a commensal in the human...Ch. 8 - Match the following terms to the definitions in...
Ch. 8 - Match the following terms to the definitions in...Ch. 8 - Feedback inhibition differs from repression...Ch. 8 - Bacteria can acquire antibiotic resistance by all...Ch. 8 - Suppose you inoculate three flasks of minimal...Ch. 8 - Plasmids differ from transposons in that plasmids...Ch. 8 - Mechanism by which the presence of glucose...Ch. 8 - The mechanism by which lactose controls the lac...Ch. 8 - Two offspring cells are most likely to inherit...Ch. 8 - Which of the following is not a method of...Ch. 8 - Nucleoside analogs and ionizing radiation are used...Ch. 8 - Replication of the E. coli chromosome takes 40 to...Ch. 8 - Pseudomonas has a plasmid containing the mer...Ch. 8 - Ciprofloxacin, erythromycin, and acyclovir are...Ch. 8 - HIV, the virus that causes AIDS, was isolated from...Ch. 8 - Human herpesvirus-8 (HHV-8) is common in parts of...
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- The presence of glucose in the medium will lead to: a) increased cAMP concentrations and reduction of lac operon gene expression b) increased cAMP concentrations and promotion of lac operon gene expression c) reduced cAMP concentrations and reduction of lac operon gene expression d) reduced cAMP concentrations and promotion of lac operon gene expressionarrow_forwardWhat would happen if the operator sequence of the lac operon contained a mutation that prevented the repressor protein from binding the operator? (Explain what would happen both in the presence and absence of lactose)arrow_forwardHow does exposing an E. coli cell to glucose affect the regulation of the lac operon via CAP? a. cAMP binds to CAP and transcription is increased. b. cAMP binds to CAP and transcription is decreased. c. cAMP does not bind to CAP and transcription is increased. d. cAMP does not bind to CAP and transcription is decreased.arrow_forward
- The gad operon is controlled by a number of transcription factors that regulate a promoter of approximately 450bp. Which would NOT explain why bacterial promoters are shorter than the typical eukaryotic promoter? a. Bacteria do not have nucleosomes b. Bacteria do not have mediator complex c. Each operon is regulated by only one signal d. All of the above are truearrow_forwardAttardi et al (1963) found out that lactose operon mRNA synthesis in E. coli increased with the addition of an inducer. Explain why the inducer act at the transcription level.arrow_forwardDescribe two examples of regulators that acted on transcription at steps after initiation. Of the two examples, one must be from a phage system and the other from a bacterial biosynthetic operon.arrow_forward
- What is the role of a substrate that controls a repressible operon? a. increase the production of inactive repressor proteins b. bind to the repressor protein and activate it c. bind to the repressor protein and deactivate it d. bind to the promoter region and decrease the affinity of RNA polymerase for the promoterarrow_forwardIf CAP is bound to the Promoter of the Lac Operon and the repressor protein is not bound to the operator, which of the following is true: a.Glucose Levels are low & Lactose is absent b.Glucose is absent & Lactose is present c.cAMP is low & Lactose is Absent d.Glucose and Lactose are Present e.cAMP is low & Lactose is presentarrow_forwardWhat if there was a mutation in the operator region of the lac operon and the active repressor could not bind there? How would this affect the cell’s production of β–galactosidase?In general, Histone acetylation makes DNA ___________________, while DNA methylation makes DNA ________________.arrow_forward
- Regarding the tryptophan operon in bacteria, in the presence of tryptophan, tryptophan binds to the Group of answer choices Operon Operator Repressor Promoter RNA polymerasearrow_forwardExplain why large amounts of transcript result when a bacterium with a lac Operon is in a medium with no glucose and an abundance of lactose.arrow_forwardWhich of the following describes the lac operon in E. coli when lactose and glucose is present in the culture medium? Select one: a. CAP inactive and RNA polymerase does not completely bind to the promoter of the lac operon and transcription of lactose-metabolizing enzymes decreases. b. CAP is active and RNA polymerase binds completely to the promoter of the lac operon and transcription of lactose-metabolizing enzymes decreases. c. Catabolite activator protein (CAP) inactive and RNA polymerase does not completely bind to the promoter of the lac operon and transcription of lactose-metabolizing enzymes increases. d. CAP is active and RNA polymerase binds completely to the promoter of the lac operon and transcription of lactose-metabolizing enzymes increases.arrow_forward
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