Microbiology: An Introduction
12th Edition
ISBN: 9780321929150
Author: Gerard J. Tortora, Berdell R. Funke, Christine L. Case
Publisher: PEARSON
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Textbook Question
Chapter 8, Problem 8MCQ
The mechanism by which lactose controls the lac operon.
Use the following choices to answer questions 7 and 8.
- a. catabolite repression
- b. DNA polymerase
- c. induction
- d. repression
- e. translation
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Explain why large amounts of transcript result when a bacterium with a lac Operon is in a medium with no glucose and an abundance of lactose.
In the lac operon system, do the inducers act at the transcription or translation level? Explain why it acts in that level in a short but concise way.
If β-galactosidase is expressed when lactose is absent, what does this suggest about the lac operon?
a)
There is a mutation in the repressor protein
b)
There is a mutation in the catabolite activator protein
c)
There is a mutation in the promoter region
d)
There is a mutation in the luxI protein
Chapter 8 Solutions
Microbiology: An Introduction
Ch. 8 - Briefly describe the components of DNA, and...Ch. 8 - DRAW IT Identify and mark each of the following on...Ch. 8 - Match the following examples of mutagens. Column A...Ch. 8 - The following is a code for a strand of DNA. a....Ch. 8 - Identify when (before transcription, after...Ch. 8 - Which sequence is the best target for damage by UV...Ch. 8 - You are provided with cultures with the following...Ch. 8 - Why are mutation and recombination important in...Ch. 8 - NAME IT Normally a commensal in the human...Ch. 8 - Match the following terms to the definitions in...
Ch. 8 - Match the following terms to the definitions in...Ch. 8 - Feedback inhibition differs from repression...Ch. 8 - Bacteria can acquire antibiotic resistance by all...Ch. 8 - Suppose you inoculate three flasks of minimal...Ch. 8 - Plasmids differ from transposons in that plasmids...Ch. 8 - Mechanism by which the presence of glucose...Ch. 8 - The mechanism by which lactose controls the lac...Ch. 8 - Two offspring cells are most likely to inherit...Ch. 8 - Which of the following is not a method of...Ch. 8 - Nucleoside analogs and ionizing radiation are used...Ch. 8 - Replication of the E. coli chromosome takes 40 to...Ch. 8 - Pseudomonas has a plasmid containing the mer...Ch. 8 - Ciprofloxacin, erythromycin, and acyclovir are...Ch. 8 - HIV, the virus that causes AIDS, was isolated from...Ch. 8 - Human herpesvirus-8 (HHV-8) is common in parts of...
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- What would occur if the repressor of an inducible operon weremutated so it could not bind the operator?(A) irreversible binding of the repressor to the promoter(B) reduced transcription of the operon’s genes(C) buildup of a substrate for the pathway controlled by theoperon(D) continuous transcription of the operon’s genesarrow_forwardUnder which of the following conditions would a lac operon produce the greatest amount of β-galactosidase? The least? Explain your reasoning. Lactose present Glucose present Condition 1 Yes No Condition 2 No Yes Condition 3 Yes Yes Condition 4 No Noarrow_forwardWhich will be bound to the Lac Operon in an environment with lactose and glucose? A) both repressor and CAP B) repressor only C) CAP only D) neither repressor nor CAParrow_forward
- The transcription of many bacterial genes relies on functional groups called operons, such as the tryptophan operon. What is an operon?arrow_forwardA researcher engineers a lac operon on a plasmid but inactivates all parts of the lac operator (lacO) and the lac promoter, replacing them with the binding site for the LexA repressor (which acts in SOS response) and a promoter regulated by LexA. The plasmid is introduced into E. coli cells that have a lac operon with an inactive lacZ gene. Under what conditions will these cells produce beta-galactosidase?arrow_forwardCompare and contrast the lac operon and trp operon of E. coli.arrow_forward
- Using the lac operon as a model, explain the role of inducers, repressors, and inducer exclusion.arrow_forwardA mutation that inactivates the repressor gene of the lac operon results in (a) the continuous transcription of the structural genes (b) no transcription of the structural genes (c) the binding of the repressor to the operator (d) no production of RNA polymerase (e) no difference in the rate of transcriptionarrow_forwardINTERPRET DATA Develop a simple hypothesis that would explain the behavior of each of the following types of mutants in E. coli. Mutant a: The map position of this mutation is in the trp operon. The mutant cells are constitutive; that is, they produce all the enzymes coded for by the trp operon, even if large amounts of tryptophan are present in the growth medium. Mutant b: The map position of this mutation is in the trp operon. The mutant cells do not produce any enzymes coded for by the trp operon under any conditions. Mutant c: The map position of this mutation is some distance from the trp operon. The mutant cells are constitutive; that is, they produce all the enzymes coded for by the trp operon, even if the growth medium contains large amounts of tryptophan.arrow_forward
- Which of the following is characteristic of genes and gene regulation in both bacteria and eukaryotes? (a) promoters (b) non-coding DNA within coding sequences (c) enhancers (d) operons (e) DNA located in a nucleusarrow_forwardThe lactose operon in E. coli comprises two structural genes and three regulatory components.a.State the name of each structural gene and describe its function: b.) State the name of each regulatory component and describe its functionarrow_forwardWhich of the statements about the attenuator region of the trp operon is TRUE? Choose an answer below: It is located upstream of the promoter. It is located upstream of the operator. Its transcript contains inverted repeats. It is located upstream of the structural genes. c and d, but not a or barrow_forward
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