Microbiology: An Introduction
12th Edition
ISBN: 9780321929150
Author: Gerard J. Tortora, Berdell R. Funke, Christine L. Case
Publisher: PEARSON
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Textbook Question
Chapter 8, Problem 8R
You are provided with cultures with the following characteristics:
Culture 1: F+, genotype A+ B+ C+
Culture 2: F−, genotype A− B− C−
- a. Indicate the possible genotypes of a recombinant cell resulting from the conjugation of cultures 1 and 2.
- b. Indicate the possible genotypes of a recombinant cell resulting from conjugation of the two cultures after the F+ has become an Hfr cell.
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Match the following Conjugation terms with the most appropriate description in the image:
F- recombinant
F- strain
F+ strain
HFR strain
An Hfr strain that is leuA+ and thiL+ was mixed with a strain thatis leuA− and thiL−. In the data points shown in the following graph,the conjugation was interrupted at different time points, and thepercentage of recombinants for each gene was determined bystreaking on a medium that lacked either leucine or thiamine.What information do you know based on the question and your understanding of the topic?
Choose the one answer that fits best. The pGLO plasmid you used contains an araC gene, a GFP gene and a bla gene. If you performed the exact same experiment with a plasmid that contained a GFP gene and a bla gene, but was missing the araC gene, what would you expect to see on your four plates?
a.
The positive control would not have any bacterial lawn
b.
The negative control would have as many colonies as the experimental plate
c.
The LB/Amp/Ara plate would have some colonies but they would not be fluorescent under UV light
d.
The experimental plate would not have any colonies
e.
The LB/Amp plate containing E. coli (+)pGLO would be fluorescent
Chapter 8 Solutions
Microbiology: An Introduction
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Ch. 8 - Match the following terms to the definitions in...Ch. 8 - Feedback inhibition differs from repression...Ch. 8 - Bacteria can acquire antibiotic resistance by all...Ch. 8 - Suppose you inoculate three flasks of minimal...Ch. 8 - Plasmids differ from transposons in that plasmids...Ch. 8 - Mechanism by which the presence of glucose...Ch. 8 - The mechanism by which lactose controls the lac...Ch. 8 - Two offspring cells are most likely to inherit...Ch. 8 - Which of the following is not a method of...Ch. 8 - Nucleoside analogs and ionizing radiation are used...Ch. 8 - Replication of the E. coli chromosome takes 40 to...Ch. 8 - Pseudomonas has a plasmid containing the mer...Ch. 8 - Ciprofloxacin, erythromycin, and acyclovir are...Ch. 8 - HIV, the virus that causes AIDS, was isolated from...Ch. 8 - Human herpesvirus-8 (HHV-8) is common in parts of...
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genetic recombination strategies of bacteria CONJUGATION, TRANSDUCTION AND TRANSFORMATION; Author: Scientist Cindy;https://www.youtube.com/watch?v=_Va8FZJEl9A;License: Standard youtube license