Precipitate protein is obtained by treating 5.00 mL sample of blood with trichloroacetic acid. The final solution has pH of 3 and this solution is extracted with two portions of 5 ml of methyl isobutyl ketone APCD that is an organic lead complex agent, this whole process is done after centrifugation. Absorbance of 0.454 at 283.3nm is obtained when the extracted solution is reacted directly with air-acetylene flame. In the same way, absorbance of 0.412 and 0.642 is obtained by treating 5mL aliquots standard solution of 0.240 ppm and 0.475 ppm of Pb. Concentration of Pb in ppm needs to be calculated by following the Beer’s law. Concept introduction: Beer Lambert law gives the relationship between absorbance and concentration of a sample. For the calculation of concentration, the following the equation of straight line as follows: y = m x + c Where, y = absorbance x = concentration of sample m is slope of graph plotted between absorbance and concentration of the sample. And, C is intercept of the graph.

BuyFind

Principles of Instrumental Analysis

7th Edition
Douglas A. Skoog + 2 others
Publisher: Cengage Learning
ISBN: 9781305577213
BuyFind

Principles of Instrumental Analysis

7th Edition
Douglas A. Skoog + 2 others
Publisher: Cengage Learning
ISBN: 9781305577213

Solutions

Chapter 9, Problem 9.20QAP
Interpretation Introduction

Interpretation:

Precipitate protein is obtained by treating 5.00 mL sample of blood with trichloroacetic acid. The final solution has pH of 3 and this solution is extracted with two portions of 5 ml of methyl isobutyl ketone APCD that is an organic lead complex agent, this whole process is done after centrifugation. Absorbance of 0.454 at 283.3nm is obtained when the extracted solution is reacted directly with air-acetylene flame. In the same way, absorbance of 0.412 and 0.642 is obtained by treating 5mL aliquots standard solution of 0.240 ppm and 0.475 ppm of Pb. Concentration of Pb in ppm needs to be calculated by following the Beer’s law.

Concept introduction:

Beer Lambert law gives the relationship between absorbance and concentration of a sample. For the calculation of concentration, the following the equation of straight line as follows:

y=mx+c

Where,

y = absorbance

x = concentration of sample

m is slope of graph plotted between absorbance and concentration of the sample.

And,

C is intercept of the graph.

Expert Solution

Want to see the full answer?

Check out a sample textbook solution.

Want to see this answer and more?

Experts are waiting 24/7 to provide step-by-step solutions in as fast as 30 minutes!*

*Response times may vary by subject and question complexity. Median response time is 34 minutes for paid subscribers and may be longer for promotional offers.