Genetics: From Genes to Genomes, 5th edition
Genetics: From Genes to Genomes, 5th edition
5th Edition
ISBN: 9780073525310
Author: Leland H. Hartwell, Michael L. Goldberg, Janice A. Fischer, Leroy Hood, Charles F. Aquadro
Publisher: McGraw-Hill Education
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Chapter 11, Problem 18P
Summary Introduction

a.

To determine:

The number of nucleotides to be added per second by DNA polymerase.

Introduction:

The building blocks of nucleic acids that are composed of a base, a sugar molecule along with a phosphate group is termed as a nucleotide.

Summary Introduction

b.

To determine:

The average rate at which DNA polymerase adds complementary nucleotides to a growing chain.

Introduction:

The synthesis of molecules of DNA from deoxyribonucleotides is carried out by the enzyme called the DNA polymerase.

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In a turtle species, a diploid cell in the G1 phase of the cell cycle contains 22 picograms of DNA (picogram is a measure of the total mass of the DNA present). How much DNA is present in a cell of the same species at prophase II of meiosis (prophase of the second meiotic division)?
What difference exists between the compaction of chromosomes during metaphase versus interphase? Give at least one reason why this difference may be necessary.
The mitotic cell divisions in the early embryo ofD. melanogaster occur very rapidly (every eightminutes).a. If there were one bidirectional origin in the middleof each chromosome, how many nucleotides wouldDNA polymerase have to add per second to replicate all the DNA in the longest chromosome(66 Mb) during the eight-minute early embryoniccell cycles? (Assume that replication occurs duringthe entire cell division cycle.)b. In fact, many origins of replication are active oneach chromosome during the early embryonic divisions and are spaced approximately 7 kb apart. Calculate the average rate (per second) with whichDNA polymerase adds complementary nucleotidesto a growing chain in the early Drosophila embryo,making the same assumption as in part (a)
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