Genetics: From Genes To Genomes (6th International Edition)
6th Edition
ISBN: 9781260041217
Author: Leland Hartwell Dr., ? Michael L. Goldberg Professor Dr., ? Janice Fischer, ? Leroy Hood Dr.
Publisher: Mcgraw-Hill
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Question
Chapter 11, Problem 18P
Summary Introduction
To determine:
The ways by which a prosecutor use DNA from the seed pods to build a case against the defendant.
Introduction:
DNA fingerprinting is defined as the method in which variable elements present in the sequence of DNA are isolated and identified. The DNA is obtained from a sample of cells such as skin, blood, or hair cells. They are a rich source of DNA.
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Draw a sketch of what they might look like
The following gel shows the results of a crime scene investigation. Lane 1 shows a DNA sample
that was obtained from the scene of a crime (there was evidence of the criminal cutting himself, so
it's the criminal's blood sample that produced the DNA bands in lane 1).
Lanes 2-7 shows a DNA sample from six potential suspects held in connection with the crime.
Arrange the bands labeled A, B, C, D from largest to smallest.
1 2 3 4 5 6 7
OC, A, D, B
A, B, C, D
OB, D, A, C
O D, A, C, B
The Evidence shows which suspect was at the crime scene? ( image of murder investigation)
A) suspect A
B) suspect B
C) suspect C
D) none were at the crime scene
Which of the following choices most likely lists the sizes of the four DNA fragments in lane 6 ( second image) of this agarose gel stained with ethidium bromide? ( Note: the well that the DNA was loaded into is at the top of the gel image).
A) 100 bp, 300 bp, 450 bp, 600 bp.
B) 300 bp, 400 bp, 100 bp, 600 bp
C) 600 bp, 450 bp, 300 bp, 100 bp
D) 400 bp, 300 bp, 600 bp, 100 bp
E) DNA fragment size can't be estimated using an agarose gel.
Chapter 11 Solutions
Genetics: From Genes To Genomes (6th International Edition)
Ch. 11 - Choose the phrase from the right column that best...Ch. 11 - Would you characterize the pattern of inheritance...Ch. 11 - Would you be more likely to find single nucleotide...Ch. 11 - A recent estimate of the rate of base...Ch. 11 - If you examine Fig. 11.5 closely, you will note...Ch. 11 - Approximately 50 million SNPs have thus far been...Ch. 11 - Mutations at simple sequence repeat SSR loci occur...Ch. 11 - Humans and gorillas last shared a common ancestor...Ch. 11 - In 2015, an international team of scientists...Ch. 11 - Using PCR, you want to amplify an approximately 1...
Ch. 11 - Prob. 11PCh. 11 - The previous problem raises several interesting...Ch. 11 - You want to make a recombinant DNA in which a PCR...Ch. 11 - You sequence a PCR product amplified from a...Ch. 11 - Prob. 15PCh. 11 - The trinucleotide repeat region of the Huntington...Ch. 11 - Sperm samples were taken from two men just...Ch. 11 - Prob. 18PCh. 11 - a. It is possible to perform DNA fingerprinting...Ch. 11 - On July 17, 1918, Tsar Nicholas II; his wife the...Ch. 11 - The figure that follows shows DNA fingerprint...Ch. 11 - Microarrays were used to determine the genotypes...Ch. 11 - A partial sequence of the wild-type HbA allele is...Ch. 11 - a. In Fig. 11.17b, PCR is performed to amplify...Ch. 11 - The following figure shows a partial microarray...Ch. 11 - Scientists were surprised to discover recently...Ch. 11 - The microarray shown in Problem 25 analyzes...Ch. 11 - The figure that follows shows the pedigree of a...Ch. 11 - One of the difficulties faced by human geneticists...Ch. 11 - Now consider a mating between consanguineous...Ch. 11 - The pedigree shown in Fig. 11.22 was crucial to...Ch. 11 - You have identified a SNP marker that in one large...Ch. 11 - The pedigrees indicated here were obtained with...Ch. 11 - Approximately 3 of the population carries a mutant...Ch. 11 - The drug ivacaftor has recently been developed to...Ch. 11 - In the high-throughput DNA sequencing protocol...Ch. 11 - A researcher sequences the whole exome of a...Ch. 11 - As explained in the text, the cause of many...Ch. 11 - Figure 11.26 portrayed the analysis of Miller...Ch. 11 - A research paper published in the summer of 2012...Ch. 11 - Table 11.2 and Fig. 11.27 together portray the...Ch. 11 - The human RefSeq of the entire first exon of a...Ch. 11 - Mutations in the HPRT1 gene in humans result in at...Ch. 11 - Prob. 44P
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- Examine the gel from a Rape investigation below. If you were the DNA analyst you would conclude that: DNA size markers blood sexual assault evidence samples victim |suspect A suspect B female fracțion male fraction 1 2 3 4 5 6 A. Suspect B is excluded as the source of the evidence, but Suspect A cannot be excluded. B. Both Suspects A and B are excluded as the source of the evidence. C. Suspect A is excluded as the source of the evidence, but Suspect B cannot be excluded. D. Suspect B cannot be excluded as a source of the evidence. The results with Suspect A are inconclusive. E. Neither Suspect A or B can be excluded as a source of the evidence.arrow_forwardBrenda is a junior student in the biomedical program at her school. She is starting the PCR genetic testing lab activity. She is about to obtain her DNA sample but doesn’t want like the taste of NaCl solution. Her friend, Mark, let her use some of his DNA. What laboratory tule did the students break? A. Obtaining and handling DNA sample without wearing googles or gloves B. Improper use of human DNA samples C. Violating Patient Confidentiality D. Disposing of bio hazardous material in a regular trasharrow_forwardIs PCR technique can help solved crimes? If yes, how? If no, why? [Explain it in 10-15 sentences]arrow_forward
- Below is an image of the results of a gel electrophoresis experiment. Lanes 1-4 contain amplified DNA fragments and Lane 5 contains the DNA size marker (DSM). From this image and by only using Lanes 1- 4 which lane matches each description below? a) This lane contains the largest DNA fragment: b) This lane contains the smallest DNA fragment: c) This lane contains a 2.5 kbp DNA fragment: 42 | || ||| | || 12 kb 9.5 kb 6.5 kb 4 kb 2 kb ***arrow_forwardDNA dragnets have been so successful that some people have suggested that DNA samples of everyone should be stored at birth, so a profile could be made for anyone at any time. A. Do you think this is a good idea or not? And, B. do you think it useful or ethical for the FBI to store DNA samples from people who have been arrested but not yet convicted of a crime? Answer both questions,arrow_forwardYou are a forensic scientist in charge of DNA samples in a case where a man was murdered. You conduct gel electrophoresis on the samples to determine the DNA profile of the samples. The rows of bands have been marked to make it easier to reference the bands. The sample marked 'evidence' came from under the victim's fingernails, likely indicating a struggle. There are two suspects in the case, both of whom are brothers, they are marked suspect 1 and suspect 2, for brother 1 and brother 2, respectfully. Does the evidence collected match the DNA profiles of either brother? If yes, which one? If no, then would it be likely that the actual criminal is a family member of the brothers? If yes, what could the relation be (mom, dad, cousin, aunt, or uncle)? Note: there is a sister in their family, their parents are both still alive, and they have a robust extended family. The brothers are not twins.arrow_forward
- In typing DNA from a sample found at a crime scene, how can a DNA mismatch prove that a suspect is not the source of that sample,whereas a DNA match does not necessarily prove that a suspect is the source?arrow_forwardPlease answer these two questions regarding PCR: a) Why do you need to perform PCR on DNA obtained from a crime scene? b) Why so forensic labs analyze non-coding DNA rather than genes?arrow_forwardWhich of the following statements regarding DNA fingerprinting is false?a) DNA fingerprinting cannot be used for paternity testingb) DNA profile using STR (short terminal repeats) is unique to an individualc) PCR is used for DNA profilingd) Forensic analysis makes use of SNPs (single nucleotide polymorphisms) in coding sequences to distinguish between individualsarrow_forward
- Discuss the principle of DNA fingerprinting. How would you use the technique to find out a criminal in forensic studies ? Explain.arrow_forwardMany times a forensic scientist has only a single hair or a single drop of blood for analysis. The amount of DNA in this kind of material is very tiny, and is not enough to use for comparison. In other words, running a single experiment to compare the DNA in a drop of blood with several suspects would use up all of the DNA in that drop of blood. This could be a serious problem if none of the suspects provided a match. How would a forensic scientist increase the amount of DNA available to them, starting with the DNA that would be found in their crime scene sample, such as a drop of blood from a suspect? PCR RFLPS Gel electrophoresis STRSarrow_forwardState the five basic steps of DNA fingerprinting using the RFLP method. Why do you think the PCR method is of more use in crime scene investigations?arrow_forward
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