GENETIC ANALYSIS: AN INTEG. APP. W/MAS
GENETIC ANALYSIS: AN INTEG. APP. W/MAS
2nd Edition
ISBN: 9781323142790
Author: Sanders
Publisher: Pearson Custom Publishing
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Chapter 12, Problem 40P

Two haploid strains of fungus are fused to form a diploid that produces eight-celled asci. Fungus strain A has the genotype + ade1 his2, and strain B is a++ . The three genes are linked and occur in the order given.

a. The alleles at the A gene locus are determined in an ascus, and the order is aaaa + + + + . Write the genotype for all three genes that you expect to find most commonly.

b. One ascus from the diploid is of the following type:

+ ade1 his2 + ade1 his2 + ade1 his2 + ade1 his2

a ade1 + a  +     + a  +     + a  +     +

Explain the events that produced this ascus.

c. One ascus from the diploid is of the following type:

a    +     + a    +     + a    +   his2 a    +   his2 + ade1 his2 + ade1 his2 + ade1 his2 + ade1 his2

Explain the events that produced this ascus.

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You cross two yeast strains one is an ade auxotroph the other is a pro auxotroph and allow the diploid to sporulate. When you score each spore in the ascus you find the following proportions: 518 PD, 8 NPD, and 225 T. a.) What are the genotypes of each spore in all three types of the tetrads. b) Are these genes linked why or why not? c.) If these genes are unlinked what would you expect the progeny numbers and ratios to be? d.) What is the formula to determine the most accurate distance between these genes? If linked what is the map distance?
Neurospora of genotype a + c are crossed withNeurospora of genotype + b +. (Here, + is shorthandfor the wild-type allele.) The following tetrads areobtained (note that the genotype of the four sporepairs in an ascus are listed, rather than listing alleight spores):a + c a b c + + c + b c a b + a + ca + c a b c a + c a b c a b + a b c+ b + + + + + b + + + + + + c + + ++ b + + + + a b + a + + + + c + b +137 141 26 25 2 3a. In how many cells has meiosis occurred to yieldthese data?b. Give the best genetic map to explain these results.Indicate all relevant genetic distances, both betweengenes and between each gene and the centromere.c. Diagram a meiosis that could give rise to oneof the three tetrads in the class at the far right inthe list
One yeast strain carries the alleles lys+ and arg+, whereas another strain has lys-3 and arg-2. The two strains were crossed toeach other, and an ascus obtained from this cross has four spores with the following genotypes: lys+ arg+, lys+ arg-2, lys-3arg+, and lys-3 arg 2. This ascus has a. a parental ditype.b. a tetratype.c. a nonparental ditype.d. either a tetratype or a nonparental ditype.

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GENETIC ANALYSIS: AN INTEG. APP. W/MAS

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