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GENETIC ANALYSIS: AN INTEG. APP. W/MAS
2nd Edition
ISBN: 9781323142790
Author: Sanders
Publisher: Pearson Custom Publishing
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Question
Chapter 12, Problem 24P
Summary Introduction
To analyze:
It is relatively easy to detect gene conversion in four-cell and eight-cell asci of fungi, where ratios such as
Introduction:
Gene conversion can be defined as a process of so-called directed DNA sequence change that occurs by base-pair mismatch repair within heteroduplex DNA. Gene conversion can switch one allele to another allele already present in a heterozygous genotype. This process is mostly detected in fungi that form ascus such as Neurospora crassa. Ascus is a sack of haploid spores that are the products of meiotic division.
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Students have asked these similar questions
Three haploid fungal mutants that require compound W for growth were isolated.
Each mutant contains a recessive allele in a single gene. Three compounds (A, B and
C) in the biosynthetic pathway to W are known, but their order in the pathway is
unknown. Each compound is tested for its ability to support the growth of each of
the three mutants. Phenotypes of all of the three mutants are shown in the following
table (“+" indicates growth, "-" indicates no growth).
A
C
W
Mutant 1
Mutant 2
Mutant 3
What would be the phenotype of a haploid mutant that contains both mutant alleles
in mutant 2 and 3? Phenotype refers to growth or absence of growth on compounds
A, B, C and WN.
O Like mutant 1
O Like mutant 2
Like mutant 3
O Like wild type
For a haploid fungus, the starting point in the biosynthesis of the amino acid
arginine is Compound X, which is always present in and absorbed from the
environment. The arginine biosynthetic pathway is:
Enzyme A
Enzyme B
Enzyme Ç
Compound X
It is know that genes encoding enzymes A and C are on two different
chromosomes.
Compound Y
Compound Z-
Arginine
A mutant strain of genotype a (lacking only enzyme A) is crossed to a mutant strain
of genotype c (lacking only enzyme C) to generate a diploid strain. Sporulation (i.e.
meiosis) is subsequently induced in the resulting diploid strain. What proportion of
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O 100%
50%
0%
25%
What percentage of the DNA sites in yeast are accessible, assuming that the fraction of sites observed for GAL4 is typical? To how many base pairs of the 12-Mb yeast genome does this percentage correspond?
Chapter 12 Solutions
GENETIC ANALYSIS: AN INTEG. APP. W/MAS
Ch. 12 - 11.1 Identify two general ways chemical mutagens...Ch. 12 - 11.2 Nitrous acid and (BU) alter DNA by different...Ch. 12 - 11.19 Using the adeninethymine base pair in this...Ch. 12 - The partial amino acid sequence of a wild-type...Ch. 12 - 5. Thymine is usually in its normal, common form....Ch. 12 - Ultraviolet (UV) radiation is mutagenic.
What...Ch. 12 - Researchers interested in studying mutation and...Ch. 12 - The effect of base - pair substitution mutations...Ch. 12 - Prob. 9PCh. 12 - 11.10 In numerous population studies of...
Ch. 12 - 11.11 Two different mutations are identified in a...Ch. 12 - 11.22 Many human genes are known to have homologs...Ch. 12 - 11.13 Answer the following questions concerning...Ch. 12 - Prob. 14PCh. 12 - Prob. 15PCh. 12 - Prob. 16PCh. 12 - 11.15 A sample of the bacterium is exposed to...Ch. 12 - 11.16 A strain of is identified as having a null...Ch. 12 - Define gene conversion and contrast it with gene...Ch. 12 - Prob. 20PCh. 12 - Prob. 21PCh. 12 - Prob. 22PCh. 12 - Prob. 23PCh. 12 - Prob. 24PCh. 12 - 25. If homologous recombination did not occur,...Ch. 12 - In this chapter, three features of genes or of DNA...Ch. 12 - Briefly compare the production of DNA double -...Ch. 12 - During mismatch repair, why is it necessary to...Ch. 12 - 11.27 Following the spill of a mixture of...Ch. 12 - A geneticist searching for mutations uses the...Ch. 12 - A wild - type culture of haploid yeast is exposed...Ch. 12 - A fragment of a wild - type polypeptide is...Ch. 12 - Prob. 33PCh. 12 - 11.40 Common baker’s yeast () is normally grown at...Ch. 12 - 11.41 The two gels illustrated below contain...Ch. 12 - Alkaptonuria is a human autosomal recessive...Ch. 12 - 11.33 In an experiment employing the methods of...Ch. 12 - Using your knowledge of DNA repair pathways choose...Ch. 12 - 11.35 Ataxia telangiectasia is a human inherited...Ch. 12 - 40. Two haploid strains of fungus are fused to...
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