CAMPBELL MASTERING BIOLOGY ACCESS>I<
CAMPBELL MASTERING BIOLOGY ACCESS>I<
18th Edition
ISBN: 9781323766286
Author: Pearson
Publisher: PEARSON
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Chapter 14.1, Problem 3CC

DRAW IT The template strand of a gene contains the sequence 3’-TTCAGTCGT-5’. Suppose that the nontemplate sequence could be transcribed instead of the template sequence. Draw the nontemplate sequence in 3’ to 5’ order. Then draw the mRNA sequence and translate it using Figure 14.6Q. (Be sure to pay attention to the 5’ and 3’ ends, remembering that the mRNA is antiparallel to the DNA strand.) Predict how well the protein synthesized from the nontemplate strand would function, if at all

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3’ TACAATGGGCGACGCGCTTCGTTTCAGATT 5’   5’ ATGTTACCCGCTGCGCGAAGCAAAGTCTAA 3’   Make vertical lines between codons to make this assignment easier to do.  Which strand is the template strand?  The first strand is the template strand as it going 3-5   Copy the template strand in mRNA.  Label the 5’ and 3’ ends.  5' AUG UUA CCC GCU GCG CGA AGC AAA GUC UAA 3” Write out the amino acid (you can use the short form) that this protein would be made of (keep in mind proteins are usually a minimum of 50 proteins.  Met-Leu-Pro-Ala-Ala-Arg-Ser-Lys-Val What do you notice about the mRNA strand compared to the non template strand of DNA?    2. What amino acid does the second codon code for on the DNA template strand?  ______Assume the 6th amino acid in the strand is changed from a T to a C. What amino acid does it code for now?  _______ What type of mutation is this?      3. Assume the 6th amino acid is changed from T to G on the DNA template strand.  What type of mutation is this?  What effect would…
The coding DNA strand of a gene has the following DNA sequence:  5’ ATGGCGACGATAATGTTGTGTGAGTGA 3’ 1) Find the sequence of the mRNA that would be made from this gene.  2) Find the amino acid protein sequence that would be made from this gene 3) A mutation occurs at position 17 of the coding DNA strand, where the T is substituted with A (count from 5’ end of coding strand). Write the resulting mRNA and protein sequences. Show all your work!
Below is the 5’–3’ strand of a double-stranded DNA molecule with the following nucleotidesequences (all belong to an exon):5’ C C T A T G C A G T G G C C A T A T T C C A A A G C A T A G C 3’  1. If the above DNA strand is the template (antisense) strand and the DNA molecule is transcribed that produced a functional mRNA. Assuming there are no mutations, the said mRNA is then brought to the site of protein synthesis,a. what would be the amino acid sequence of the synthesized polypeptide chain?b. how many possible kinds of tRNA molecule that will bring the 2nd amino acid observing wobble hypothesis? List down their anticodons.

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