Biology
12th Edition
ISBN: 9780134813448
Author: Audesirk, Teresa, Gerald, Byers, Bruce E.
Publisher: Pearson,
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Chapter 14.5, Problem 1CSC
Many countries regulate the use of genetically modified organisms, and enforcing the regulations requires methods of distinguishing modified and unmodified organisms whose outward appearances may be very similar. Some of the most effective methods are similar to the DNA profiling used in criminal investigations. For example, investigators in Europe routinely test crop samples by using PCR of target loci followed by gel electrophoresis to produce DNA profiles much like those used to identify criminals. These profiles are compared to those in a database of profiles from samples known to be either normal or genetically modified.
What kinds of genetically modified organisms are the European investigators trying to detect?
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Chapter 14 Solutions
Biology
Ch. 14.1 - define biotechnology?Ch. 14.1 - Prob. 2CYLCh. 14.1 - define GMO and transgenic organism?Ch. 14.2 - describe natural processes that recombine DNA,...Ch. 14.3 - Prob. 1CSCCh. 14.3 - Prob. 1CYLCh. 14.3 - summarize how CRISPR-Cas9 works and explain why it...Ch. 14.4 - For any single person, a given STR always has...Ch. 14.4 - There are many other applications in which DNA...Ch. 14.4 - Prob. 1CYL
Ch. 14.4 - Prob. 2CYLCh. 14.5 - Restriction enzymes are isolated from bacteria....Ch. 14.5 - Many countries regulate the use of genetically...Ch. 14.5 - explain how genes are inserted into a plasmid, and...Ch. 14.5 - Prob. 2CYLCh. 14.6 - Prob. 1CTCh. 14.6 - Prob. 1HYEWCh. 14.6 - describe the advantages of genetically modified...Ch. 14.6 - list some examples of how genetically modified...Ch. 14.6 - Prob. 3CYLCh. 14.7 - Explain how fetal DNA could be used to establish...Ch. 14.7 - explain how biotechnology is used to diagnose both...Ch. 14.7 - describe how transgenic organisms are used to...Ch. 14.7 - describe the procedures and advantages of gene...Ch. 14.8 - explain why people might be opposed to the use of...Ch. 14.8 - Prob. 2CYLCh. 14.8 - Prob. 1CTCh. 14 - Prob. 1MCCh. 14 - Prob. 3MCCh. 14 - A restriction enzyme a. cuts DNA at a specific...Ch. 14 - Prob. 5MCCh. 14 - Prob. 1FIBCh. 14 - _________is the process whereby bacteria pick up...Ch. 14 - The _______ is a technique tor multiplying DNA in...Ch. 14 - Matching DNA samples in forensics uses a specific...Ch. 14 - Prob. 5FIBCh. 14 - Describe two natural forms of genetic...Ch. 14 - Prob. 2RQCh. 14 - Prob. 3RQCh. 14 - Prob. 4RQCh. 14 - Prob. 5RQCh. 14 - Prob. 6RQCh. 14 - How does gel electrophoresis separate pieces of...Ch. 14 - Prob. 8RQCh. 14 - Prob. 9RQCh. 14 - Prob. 10RQCh. 14 - As you may know, many Insects have evolved...Ch. 14 - Prob. 2AC
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Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.Similar questions
- You are performing an experiment using CRISPR-cas9 to genetically modify the LacZ gene of a culture of E. coli. After you run the experiment, you decide to use gel electrophoresis to genotype the different bacterial cultures to determine if the gene editing was successful. How could your electrophoresis results confirm that the PCR was successful? And how could your electrophoresis results confirm that you successfully extracted genomic DNA from your bacterial samples?arrow_forwardWhy are entire genomes not used for DNA profiling?arrow_forwardDNA extraction, PCR, gel electrophoresis, and DNA sequencing of PCR products. Give a description of these methods and the materials required to perform each of these molecular techniquesarrow_forward
- An important feature of restriction enzymes is that each enzyme only recognizes a specific palindrome and cuts the DNA only at that specific sequence of bases. A palindromic sequence can be repeated a number of times on a strand of DNA, and the specific restriction enzyme will cut all those palindromes, no matter what species the DNA comes from. A linear DNA molecule is represented below. The DNA is represented by one line, although in actuality, DNA has two strands. If the DNA molecule has two restriction sites, specifically two repeats of a specific palindrome sequence, A and B, for a specific restriction enzyme: How many fragments would be produced if the DNA is cut by that enzyme? Number each fragment Which fragment would be the largest? Which fragment would be the smallest?arrow_forwardPCR is a powerful technique to screen and amplify segments of DNA for use in recombinant protein technologies. Describe in detail, the components of a PCR reaction and why they are requiredarrow_forwardWhat is the purpose of adding a primer to a PCR reaction? Is this primer made up of DNA or RNA nucleotides? Explain your reasoning.arrow_forward
- A student is trying to add 15.0 ng of DNA template to a 20.0 µL PCR. The DNA template is at a concentration of 65.0 ng µL-1, and the student determines that a serial dilution is required because directly adding the DNA template would require a volume less than 1.00 µL. The student wants to prepare an intermediate solution at a concentration of 15.0 ng µL-1. If the DNA template stock will be mixed with 13.0 µL of ultrapure H2O, calculate the volume (in µL) of the DNA template required to prepare the intermediate solution.arrow_forwardDescribe at least one important application of DNA technology in each of the following fields: medicine, DNA fingerprinting, and transgenic organisms.arrow_forwardExplain how biotechnology and bioinformatics are used to identify microorganisms Explain why biotechnology has replaced many of the traditional diagnostic tools in microbiology Explain why a PCR reaction needs a source of nucleotides in the form of ATP, GTP, CTP and TTP. Explain what primers are and describe their use in PCR reactions Given the results from a PCR/Gel electrophoresis or a DNA sequence; determine the identity of a bacterium or virus...arrow_forward
- Which of the following describes an advantage of using a recombinant plasmid for DNA cloning over PCR? A. PCR is more likely to have errors introduced in the copying process. B. Recombinant DNA plasmids are able to create large amounts of copies more quickly than PCR. C. PCR can only be conducted in eukaryotic cells. D. PCR requires prior knowledge of the sequence in question, while a recombinant plasmid does not.arrow_forwardExplain why a quantitative PCR analysis cannot determine the size of the initial template sequence in a DNA sample.arrow_forwardCRISPR techniques allow scientists to modify specific genes while sparing all others, thus clarifying the association between a given gene and its consequence to the organism. If this technology can change the future of Medicine, what specific benefits CRISPR can bring to genetic testing or analysis? How can CRISPR help to enhance gene therapy or treatment of genetic diseases?arrow_forward
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