Biochemistry: The Molecular Basis of Life
6th Edition
ISBN: 9780190209896
Author: Trudy McKee, James R. McKee
Publisher: Oxford University Press
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Chapter 18, Problem 71TQ
Summary Introduction
To review:
The way through which activity of reverse transcriptase results in amplification of the genes.
Introduction:
Gene amplification is the process through which a gene is amplified either in vivo or in vitro. In in vitro gene amplification, DNA (deoxyribonucleic acid) polymerase, single-strand binding proteins, DNA ligase, topoisomerases, and deoxyribonucleotides are required.
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Question 2:
Part a: Complete the table describing different components of intron removal from mRNA. Nu:, X and Y refer to B-type chemistry shown on the previous page. (YELLOW table shown)
Part b: Complete the table describing different components of group I self-splicing intron removal from 26S rRNA in Tetrahymena. (BLUE table shown)
Part c: Draw the intron with an all atom structure for Branchpoint A after intron removal from mRNA
Part d: Draw the Group I self-splicing intron with an all atom structure for the Guanosine cofactor after intron removal from 26S rRNA in Tetrahymena.
Chapter 18 Solutions
Biochemistry: The Molecular Basis of Life
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