Biochemistry: The Molecular Basis of Life
6th Edition
ISBN: 9780190209896
Author: Trudy McKee, James R. McKee
Publisher: Oxford University Press
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Chapter 18, Problem 70TQ
Summary Introduction
To review:
The major differences between the effects of errors made during replication and transcription on a person.
Introduction:
The term mutation can be defined as the permanent change in the sequence of
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QUESTION NO. 1 Base excision repair
A. is used only for bases that have been deaminated.
B. uses enzymes called DNA glycosylases to generate an abasic sugar site.
C. removes about 10 to 15 nucleotides.
D. does not require an endonuclease.
E. recognizes a bulky lesion.
QUESTION NO. 2
Termination of a prokaryotic transcriptA. is a random process.
B. requires the presence of the rho subunit of the holoenzyme.
C. does not require rho factor if the end of the gene contains a G-C rich palindrome.
D. is most efficient if there is an A-T-rich segment at the end of the gene.
E. requires an ATPase in addition to rho factor.
QUESTION NO. 3
Eukaryotic transcription
A. is independent of the presence of upstream consensus sequences.
B. may involve a promoter located within the region transcribed rather than upstream.
C. requires a separate promoter region for each of the three ribosomal RNAs transcribed.
D. requires that…
What feature of eukaryotic translation is especially responsible for its efficiency?
What is the real definition of DNA ligase to make it true
Chapter 18 Solutions
Biochemistry: The Molecular Basis of Life
Ch. 18 - Prob. 1QCh. 18 - Prob. 2QCh. 18 - Prob. 3QCh. 18 - Prob. 4QCh. 18 - Prob. 5QCh. 18 - Prob. 1RQCh. 18 - Prob. 2RQCh. 18 - Prob. 3RQCh. 18 - Prob. 4RQCh. 18 - Prob. 5RQ
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- As we focused on the genetic code and the transcription of genetic information stored in DNA into complementary RNA molecules. Along the way, we found many opportunities to consider the methods and reasoning by which much of this information was acquired. From the explanations given in the chapter, what answers would you propose to the following fundamental questions: Question: How were the experimentally derived triplet codon assignments verified in studies using bacteriophage MS2?arrow_forwardWhat are the Okazaki fragments?arrow_forwardWhat are Okazaki fragments? Why are they formed?arrow_forward
- why DNA polymerase cannot remove all the RNA primers from Okazaki fragments to form a lagging strand. Explain.arrow_forwardWhat is the difference between eukaryotes and prokaryotes in terms of translation initiation?arrow_forwardTrue or False: there are three different start codons in the genetic codearrow_forward
- Question 14 A large RNA–protein complex responsible for RNA splicing is called: Question 14 options: Spliceosome Splicing complex Exon-intron processor Transcription complex Question 15 Shortly after transcriptional initiation by RNA polymerase II, a methylated nucleoside (7-methylguanosine, m7G) is added and linked by a 5′–5′ phosphodiester bond to the first 5′ nucleotide, What is this process called? Question 15 options: 3' Capping 5' Capping Polyadenylation Splicing Question 16 During polyadenylation, the RNA is cleaved at a specific site. The site is: Question 16 options: 15–30 nucleotides downstream of the AAUAAA sequence 15–30 nucleotides upstream of the AAUAAA sequence at AAUAA sequence At…arrow_forwardTopic is central dogma of molecular biology Question: 4. Assuming the translation product is an enzyme, explain its role in the final expression of a phenotype. Please explain it to me thank youarrow_forwardQuestion 1. Although we will not be doing a gel electrophoresis, data from a gel digest of a Bacillus anthrax plasmid is provided so you can do a DNA map. The Bacillus anthrax plasmid is 4000bp (4Kb) long. Note the origin position as well as the reference molecular weight markers on the gel. Two restriction enzymes, A and B, were used to obtain two individual digests, A and B. They were combined to produce the third digest. The restriction enzyme fragment pattern for the digest of Bacillus anthrax plasmid Determining the Number of Fragments How many fragments were produced by enzyme A? How many fragments were produced by enzyme B? How many fragments were produced by the combined digest (A and B)? Fragment Size Fragment size is relative to molecular weight, and must be determined by comparing the fragment distance to the molecular weight markers. The fragment size has been provided on the gel pattern for this exercise. To make a map you must determine the relative positions of the…arrow_forward
- Adenine is a niterogenous base or nucleosides?arrow_forwardFollowing base removal, DNA polymerase can add nucleotides in the 5'-to-3' direction. Is that true or False? Why? please help me explain thatarrow_forwardQUESTION 24 During lagging strand synthesis of DNA, Okazaki fragments are linked together by ___________. DNA polymerase I Primase Beta clamps DNA Ligasearrow_forward
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