(a) Interpretation: The type of the radioactive material needs to be determined that is added to the culture medium considering that DNA is supposed to be radioactively labeled but not RNA, in dividing and growing bacterial cell. Concept introduction: Nucleotides are organic molecules which act as monomer cells for the formation of deoxyribonucleic acid (DNA) and ribonucleic acid (RNA) nucleic acid structures, both of which are vital biomolecules in all life forms on Earth. Nucleotides are the construction buildings of nucleic acids ; they consist of three sub-unit molecules: a core of nitrogen (also recognized as nucleobase), five-carbon acid (ribose or deoxyribose) and at least one set of phosphates.

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Answer 1

Question

Chapter 4, Problem 19P

Interpretation Introduction

(a)

Interpretation:

The type of the radioactive material needs to be determined that is added to the culture medium considering that DNA is supposed to be radioactively labeled but not RNA, in dividing and growing bacterial cell.

Concept introduction:

Nucleotides are organic molecules which act as monomer cells for the formation of deoxyribonucleic acid (DNA) and ribonucleic acid (RNA) nucleic acid structures, both of which are vital biomolecules in all life forms on Earth.

Nucleotides are the construction buildings of nucleic acids; they consist of three sub-unit molecules: a core of nitrogen (also recognized as nucleobase), five-carbon acid (ribose or deoxyribose) and at least one set of phosphates.

Interpretation Introduction

(b)

Interpretation:

A product comprising DNA polymerase and primed model DNA must be supplemented with precursors. Also, where core phosphorous atoms are evenly marked with 32P, the location of radioactive atoms in these precursors when the DNA is to be ready should be stated.

Concept introduction:

DNA elongation performs in 5’-3’direction. Chain elongation reaction takes place when 3-hydroxyl group attacks the innermost phosphorus atom of deoxy nucleoside triphosphate. In the DNA replication method where the process is associated with the nuclear matrix, precursors are injected. DNA polymerases catalyze the gradual introduction to a DNA sequence of deoxyribonucleotide components.

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Students have asked these similar questions

Preparing plasmid DNA (double stranded, circular) for Sanger sequencing involves annealing a complementary, single-stranded oligonucleotide DNA primer to one strand of the plasmid template. This is routinely accomplished by heating the plasmid DNA and primer to 90°C and then slowly bringing the temperature down to 25°C. Why does this protocol work? What enzyme is used and what other components are required in the sequencing reaction? How does the Sanger method determine the sequence?

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Answer 2

Question

Chapter 4, Problem 19P

Interpretation Introduction

(a)

Interpretation:

The type of the radioactive material needs to be determined that is added to the culture medium considering that DNA is supposed to be radioactively labeled but not RNA, in dividing and growing bacterial cell.

Concept introduction:

Nucleotides are organic molecules which act as monomer cells for the formation of deoxyribonucleic acid (DNA) and ribonucleic acid (RNA) nucleic acid structures, both of which are vital biomolecules in all life forms on Earth.

Nucleotides are the construction buildings of nucleic acids; they consist of three sub-unit molecules: a core of nitrogen (also recognized as nucleobase), five-carbon acid (ribose or deoxyribose) and at least one set of phosphates.

Interpretation Introduction

(b)

Interpretation:

A product comprising DNA polymerase and primed model DNA must be supplemented with precursors. Also, where core phosphorous atoms are evenly marked with 32P, the location of radioactive atoms in these precursors when the DNA is to be ready should be stated.

Concept introduction:

DNA elongation performs in 5’-3’direction. Chain elongation reaction takes place when 3-hydroxyl group attacks the innermost phosphorus atom of deoxy nucleoside triphosphate. In the DNA replication method where the process is associated with the nuclear matrix, precursors are injected. DNA polymerases catalyze the gradual introduction to a DNA sequence of deoxyribonucleotide components.

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Answer 3

Question

Chapter 4, Problem 19P

Interpretation Introduction

(a)

Interpretation:

The type of the radioactive material needs to be determined that is added to the culture medium considering that DNA is supposed to be radioactively labeled but not RNA, in dividing and growing bacterial cell.

Concept introduction:

Nucleotides are organic molecules which act as monomer cells for the formation of deoxyribonucleic acid (DNA) and ribonucleic acid (RNA) nucleic acid structures, both of which are vital biomolecules in all life forms on Earth.

Nucleotides are the construction buildings of nucleic acids; they consist of three sub-unit molecules: a core of nitrogen (also recognized as nucleobase), five-carbon acid (ribose or deoxyribose) and at least one set of phosphates.

Interpretation Introduction

(b)

Interpretation:

A product comprising DNA polymerase and primed model DNA must be supplemented with precursors. Also, where core phosphorous atoms are evenly marked with 32P, the location of radioactive atoms in these precursors when the DNA is to be ready should be stated.

Concept introduction:

DNA elongation performs in 5’-3’direction. Chain elongation reaction takes place when 3-hydroxyl group attacks the innermost phosphorus atom of deoxy nucleoside triphosphate. In the DNA replication method where the process is associated with the nuclear matrix, precursors are injected. DNA polymerases catalyze the gradual introduction to a DNA sequence of deoxyribonucleotide components.

Expert Solution & Answer

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Answer 4

Question

Chapter 4, Problem 19P

Interpretation Introduction

(a)

Interpretation:

The type of the radioactive material needs to be determined that is added to the culture medium considering that DNA is supposed to be radioactively labeled but not RNA, in dividing and growing bacterial cell.

Concept introduction:

Nucleotides are organic molecules which act as monomer cells for the formation of deoxyribonucleic acid (DNA) and ribonucleic acid (RNA) nucleic acid structures, both of which are vital biomolecules in all life forms on Earth.

Nucleotides are the construction buildings of nucleic acids; they consist of three sub-unit molecules: a core of nitrogen (also recognized as nucleobase), five-carbon acid (ribose or deoxyribose) and at least one set of phosphates.

Interpretation Introduction

(b)

Interpretation:

A product comprising DNA polymerase and primed model DNA must be supplemented with precursors. Also, where core phosphorous atoms are evenly marked with 32P, the location of radioactive atoms in these precursors when the DNA is to be ready should be stated.

Concept introduction:

DNA elongation performs in 5’-3’direction. Chain elongation reaction takes place when 3-hydroxyl group attacks the innermost phosphorus atom of deoxy nucleoside triphosphate. In the DNA replication method where the process is associated with the nuclear matrix, precursors are injected. DNA polymerases catalyze the gradual introduction to a DNA sequence of deoxyribonucleotide components.

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Answer 5

Chapter 4, Problem 19P

Interpretation Introduction

(a)

Interpretation:

The type of the radioactive material needs to be determined that is added to the culture medium considering that DNA is supposed to be radioactively labeled but not RNA, in dividing and growing bacterial cell.

Concept introduction:

Nucleotides are organic molecules which act as monomer cells for the formation of deoxyribonucleic acid (DNA) and ribonucleic acid (RNA) nucleic acid structures, both of which are vital biomolecules in all life forms on Earth.

Nucleotides are the construction buildings of nucleic acids; they consist of three sub-unit molecules: a core of nitrogen (also recognized as nucleobase), five-carbon acid (ribose or deoxyribose) and at least one set of phosphates.

Interpretation Introduction

(b)

Interpretation:

A product comprising DNA polymerase and primed model DNA must be supplemented with precursors. Also, where core phosphorous atoms are evenly marked with 32P, the location of radioactive atoms in these precursors when the DNA is to be ready should be stated.

Concept introduction:

DNA elongation performs in 5’-3’direction. Chain elongation reaction takes place when 3-hydroxyl group attacks the innermost phosphorus atom of deoxy nucleoside triphosphate. In the DNA replication method where the process is associated with the nuclear matrix, precursors are injected. DNA polymerases catalyze the gradual introduction to a DNA sequence of deoxyribonucleotide components.

Answer 6

Chapter 4, Problem 19P

Interpretation Introduction

(a)

Interpretation:

The type of the radioactive material needs to be determined that is added to the culture medium considering that DNA is supposed to be radioactively labeled but not RNA, in dividing and growing bacterial cell.

Concept introduction:

Nucleotides are organic molecules which act as monomer cells for the formation of deoxyribonucleic acid (DNA) and ribonucleic acid (RNA) nucleic acid structures, both of which are vital biomolecules in all life forms on Earth.

Nucleotides are the construction buildings of nucleic acids; they consist of three sub-unit molecules: a core of nitrogen (also recognized as nucleobase), five-carbon acid (ribose or deoxyribose) and at least one set of phosphates.

Answer 7

Chapter 4, Problem 19P

Interpretation Introduction

(a)

Interpretation:

The type of the radioactive material needs to be determined that is added to the culture medium considering that DNA is supposed to be radioactively labeled but not RNA, in dividing and growing bacterial cell.

Concept introduction:

Nucleotides are organic molecules which act as monomer cells for the formation of deoxyribonucleic acid (DNA) and ribonucleic acid (RNA) nucleic acid structures, both of which are vital biomolecules in all life forms on Earth.

Nucleotides are the construction buildings of nucleic acids; they consist of three sub-unit molecules: a core of nitrogen (also recognized as nucleobase), five-carbon acid (ribose or deoxyribose) and at least one set of phosphates.

Answer 8

Interpretation Introduction

(b)

Interpretation:

A product comprising DNA polymerase and primed model DNA must be supplemented with precursors. Also, where core phosphorous atoms are evenly marked with 32P, the location of radioactive atoms in these precursors when the DNA is to be ready should be stated.

Concept introduction:

DNA elongation performs in 5’-3’direction. Chain elongation reaction takes place when 3-hydroxyl group attacks the innermost phosphorus atom of deoxy nucleoside triphosphate. In the DNA replication method where the process is associated with the nuclear matrix, precursors are injected. DNA polymerases catalyze the gradual introduction to a DNA sequence of deoxyribonucleotide components.

Answer 9

Interpretation Introduction

(b)

Interpretation:

A product comprising DNA polymerase and primed model DNA must be supplemented with precursors. Also, where core phosphorous atoms are evenly marked with 32P, the location of radioactive atoms in these precursors when the DNA is to be ready should be stated.

Concept introduction:

DNA elongation performs in 5’-3’direction. Chain elongation reaction takes place when 3-hydroxyl group attacks the innermost phosphorus atom of deoxy nucleoside triphosphate. In the DNA replication method where the process is associated with the nuclear matrix, precursors are injected. DNA polymerases catalyze the gradual introduction to a DNA sequence of deoxyribonucleotide components.

Answer 10

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Answer 11

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Answer 12

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Answer 13

Ch. 4 - Prob. 1P Ch. 4 - Prob. 2P Ch. 4 - Prob. 3P Ch. 4 - Prob. 4P Ch. 4 - Prob. 5P Ch. 4 - Prob. 6P Ch. 4 - Prob. 7P Ch. 4 - Prob. 8P Ch. 4 - Prob. 9P Ch. 4 - Prob. 10P

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