Biochemistry (Looseleaf)
Biochemistry (Looseleaf)
9th Edition
ISBN: 9781319114800
Author: BERG
Publisher: MAC HIGHER
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Chapter 4, Problem 9P
Interpretation Introduction

Interpretation:

The minimum length of a DNA sequence that will appear only once in the human genome needs to be determined, if the human genome contains 3 billion nucleotides arranged in a vast array of the sequence. This is to be taken into account that only one strand and all four nucleotides have the same probability of appearance.

Concept introduction:

Deoxyribonucleic acid (DNA) is a molecule consisting of two strands that twist around each other to create a double strand DNA containing directions for the development, working, growth and breeding of all recognized species and many bacteria. It is also recognized as polynucleotides. The two DNA sections consist of simpler monomeric blocks called nucleotides.

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Concept 23. A gene is a discrete sequence of DNA nucleotides.                      This concept describes the discovery of the DNA sequencing technique that made modern biology and bioinformatics possible. The method was later used to sequence the human genome and genomes of many other organisms. This led to the accumulation of great numbers of nucleotide and protein sequences in the numerous databases. The concept 23 web page is here: http://www.dnaftb.org/23/   After reading the Concept page, answer the following questions. What was the Mendel’s definition of a gene? How was it different from the definition by Beadle and Tatum? Describe proteins based on the early sequencing efforts. What was the definition of a protein coding gene based on the genetic code? What are the beginning and ending codons of the gene’s protein coding sequence? What is the name of the method that makes use of a “defective” DNA nucleotide?   After reviewing the Animation pages, answer the following…
For some DNAS it is possible to separate the two strands, after denaturation, in a CsCl gradient. (a) What property of any DNA determines where it will band in a CsCI gradient? (b) What kind of DNA might have two strands that differ sufficiently in this property that they could be separated after denaturation?
For some DNAs, it is possible to separate the two strands, after denaturation,in a CsCl gradient.(a) What property of any DNA determines where it will band in a CsClgradient?(b) What kind of DNA might have two strands that differ sufficiently in thisproperty that they could be separated after denaturation?
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