Study Guide And Solutions Manual For Genetic Analysis: An Integrated Approach
3rd Edition
ISBN: 9780134832258
Author: Mark F. Sanders, John L. Bowman, Peter Mirabito
Publisher: PEARSON
expand_more
expand_more
format_list_bulleted
Question
Chapter 6, Problem 25P
Summary Introduction
To analyze:
Analyze the term genetic complementation, and answer the following questions.
Relate how the term genetic complementation applies to an experiment in which two lysis-defective bacteriophages are able to co-infect a bacterial cell and produce lysis.
Find out another example of complementation in this book, and describe how the genetic complementation applies to this case.
c. Analyze if the genetic complementation has the same meaning in both cases. If yes, explain how.
Introduction:
The term genetic complementation of two genes suggests that the two mutated genes having a mutation at two different locations produce wild type character, therefore completing each other. Complementation is also called Cis-trans mutation. Genetic complementation is used to determine whether mutation has occurred in the same gene or in two different genes.
Expert Solution & Answer
Want to see the full answer?
Check out a sample textbook solution
Students have asked these similar questions
In your own words, explain why this procedure is referred to as an enzyme-linked immunosorbent assay.
. Susceptibility of the bacteria will be determined by observing for the production of plaques. Describe how these plaques are formed. Would the different strains/species of bacteria be susceptible to bacteriophage T2? Explain why b. How does a viral plaque compares to a bacterial colony? c. Which organism is identified by the use of bacitracin? What does the absence of bacterial growth around the bacitracin disc indicate?
Draw and label the E. coli host cell/T4 bacteriophage interaction.?
Chapter 6 Solutions
Study Guide And Solutions Manual For Genetic Analysis: An Integrated Approach
Ch. 6 - For bacteria that are F+, Hfr, F', and F-, perform...Ch. 6 - The flow diagram identifies relationships between...Ch. 6 - Conjugation between an Hfr cell and an F-cell does...Ch. 6 - Bacteria transfer genes by conjugation,...Ch. 6 - Explain the importance of the following features...Ch. 6 - Prob. 6PCh. 6 - Describe what is meant by the term site-specific...Ch. 6 - What is a prophage, and how is a prophage formed?Ch. 6 - How is the frequency of cotransduction related to...Ch. 6 - Describe the differences between genetic...
Ch. 6 - Among the mechanisms of gene transfer in bacteria,...Ch. 6 - What is lateral gene transfer? How might it take...Ch. 6 - Lateral gene transfer is thought to have played a...Ch. 6 - Prob. 14PCh. 6 - A 2013 CDC report identified the practice of...Ch. 6 - Hfr strains that differ in integrated F factor...Ch. 6 - Five Hfr strains from the same bacterial species...Ch. 6 - An interrupted mating study is carried out on Hfr...Ch. 6 - An Hfr strain with the genotype cys+leu+met+strS...Ch. 6 - A triple-auxotrophic strain of E. coli having the...Ch. 6 - Penicillin was first used in the 1940 s to treat...Ch. 6 - An attribute of growth behavior of eight...Ch. 6 - Synthesis of the amino acid histidine is a...Ch. 6 - The phage P1 is used as a generalized transducing...Ch. 6 - Prob. 25PCh. 6 - Prob. 26PCh. 6 - Look closely at the consolidated Hfr map and the...Ch. 6 - Fifty bacterial colonies are on a complete-medium...
Knowledge Booster
Similar questions
- Describe the differences between genetic complementation and recombination as they relate to the detection of wild-type lysis by a mutant bacteriophage.arrow_forwardWhat is the purpose of blocking the Western Blot membrane? A) To cover the membrane with a solution of proteins that serve as a substrate for secondary antibody binding B) To maintain target proteins in their native, non-denatured state C) To cover the membrane with a solution of proteins in order to reduce non-specific binding of the antibody to the membrane D) To enhance non-specific background staining through interactions with the proteins present in the blocking solutionarrow_forwardTo understand the genes responsible for growth and infectivity in a disease-causing bacterial strain, you perform chemical mutagenesis on a culture of these bacteria. In the course of your investigation into the properties of the resulting mutants, you identify a set of mutant bacteria that is still viable but their virulence is significantly impaired. How might these mutants be useful for vaccine development?arrow_forward
- In relation to immunotechnology, answer the following: Give an example of murine Monocolonal Antibody produced by hybridoma technology?arrow_forwardTo probe for presence tubulin in protein samples by western blot you use a anti tubulin monoclonal antibody. Which of the following secondary antibody should be used? one produced by a sheep immunized with goat antibodies. one produced by a donkey immunized with rabbit antibodies one produced by a goat immunized with mouse antibodies one produced by a frog immunized with hamster antibodiesarrow_forwarda. List the bacteria from this chapter for which general, routine vaccines are given. b. For which special groups of bacteria are there vaccines? c. For which bacteria is there none?arrow_forward
- Explain why IgG subclasses is most efficient at activating complement?arrow_forwardBorrelia hermsii is a spirochete bacterium, transmitted by tick bites, that causes an illness characterized by a relapsing fever. The bacteria enter the host bloodstream and replicate there. Studies in mice show that episodes of bacteremia (bacteria in the blood) are efficiently controlled by anti-bacterial antibodies, but interestingly, follicular B cells are not required for this response, nor is the response impaired by splenectomizing the mice (i.e., removing the spleen). Which B cells are most likely responsible for this antibody response?arrow_forwardIn (b), why would it be more efficient to use labeled anti-human IgG rather than label the patient’s antibodies?arrow_forward
- Rarely, the conjugation of Hfr and F− cells produces two Hfr cells. Explain how this event takes place.arrow_forwardWith regard to the experiment described in Figure shown, Explain why an antibody was used to remove the bacteria thatwere not transformed. What would the results look like, in allfive cases, if the antibody/centrifugation step had not beenincluded in the experimental procedure?arrow_forwardExplain what is the agglutination reaction: WHAT agglutinates with what? Agglutination is the positive or negative result? WHY?arrow_forward
arrow_back_ios
SEE MORE QUESTIONS
arrow_forward_ios
Recommended textbooks for you
