Concept explainers
The phage P1 is used as a generalized transducing phage in an experiment combining a donor strain of E. coli of genotype leu+phe+ala+ and a recipient strain that is leu-phe-ala-. In separate experiments, transductants are selected for leu+ (experiment A), for phe+ (experiment B), and for ala+ (experiment C). Following selection, transductant genotypes for the unselected markers are identified.
a. What compound or compounds are added to the minimal medium to select for transductants in experiments A, B, and C?
b. Selection experiment results below show the frequency of each genotype.
c. Determine the order of genes on the donor chromosome.
d. Diagram the crossover events that form each of the transductants in experiment A.
e. In experiment B, why are there no transductants with the genotype leu- ala+?
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Study Guide And Solutions Manual For Genetic Analysis: An Integrated Approach
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