Study Guide And Solutions Manual For Genetic Analysis: An Integrated Approach
3rd Edition
ISBN: 9780134832258
Author: Mark F. Sanders, John L. Bowman, Peter Mirabito
Publisher: PEARSON
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Textbook Question
Chapter 6, Problem 5P
Explain the importance of the following features in conjugating donor bacteria:
a. the origin of transfer
b. the conjugation pilus
c. homologous recombination
d. the relaxosome
e. relaxase
f. T strand DNA
g. pilin protein
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For bacteria that are F+, Hfr, F', and F- answer the following.
a. Describe the state of the F factor.
b. Which of these cells are donors? Which is the recipient?
c. Which of these donors can convert exconjugants to a donor state?
d. Which of these donors can transfer a donor gene to exconjugants?
e. Describe the results of conjugation (i.e., changes in the recipient and the exconjugant) that allow detection of the state of the F factor in a donor strain.
f. Describe a "partial diploid" and how it originates.
Explain how a U-tube apparatus can distinguish between genetic transfer involving conjugation and genetic transfer involving transduction. Do you think a U-tube could be used to distinguish between transduction and transformation?
Define the following terms: a. nonreplicative transposition b. transposable element c. bacterial transformation d. transduction e. conjugation
Chapter 6 Solutions
Study Guide And Solutions Manual For Genetic Analysis: An Integrated Approach
Ch. 6 - For bacteria that are F+, Hfr, F', and F-, perform...Ch. 6 - The flow diagram identifies relationships between...Ch. 6 - Conjugation between an Hfr cell and an F-cell does...Ch. 6 - Bacteria transfer genes by conjugation,...Ch. 6 - Explain the importance of the following features...Ch. 6 - Prob. 6PCh. 6 - Describe what is meant by the term site-specific...Ch. 6 - What is a prophage, and how is a prophage formed?Ch. 6 - How is the frequency of cotransduction related to...Ch. 6 - Describe the differences between genetic...
Ch. 6 - Among the mechanisms of gene transfer in bacteria,...Ch. 6 - What is lateral gene transfer? How might it take...Ch. 6 - Lateral gene transfer is thought to have played a...Ch. 6 - Prob. 14PCh. 6 - A 2013 CDC report identified the practice of...Ch. 6 - Hfr strains that differ in integrated F factor...Ch. 6 - Five Hfr strains from the same bacterial species...Ch. 6 - An interrupted mating study is carried out on Hfr...Ch. 6 - An Hfr strain with the genotype cys+leu+met+strS...Ch. 6 - A triple-auxotrophic strain of E. coli having the...Ch. 6 - Penicillin was first used in the 1940 s to treat...Ch. 6 - An attribute of growth behavior of eight...Ch. 6 - Synthesis of the amino acid histidine is a...Ch. 6 - The phage P1 is used as a generalized transducing...Ch. 6 - Prob. 25PCh. 6 - Prob. 26PCh. 6 - Look closely at the consolidated Hfr map and the...Ch. 6 - Fifty bacterial colonies are on a complete-medium...
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Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.Similar questions
- Explain the difference between the two bacteriophagereplication pathwaysarrow_forwardA group of genetics students mix two auxotrophic strains of bacteria: one is leu+ trp+ his− met− and the other is leu− trp− his+ met+. After mixing the two strains, they plate the bacteria on minimal medium and observe a few prototrophic colonies (leu+ trp+ his+ met+). They assume that some gene transfer has taken place between the two strains. How can they determine whether the transfer of genes is due to conjugation, transduction, or transformation?arrow_forwardExplain the mechanism used to transfer genes via conjugation.arrow_forward
- Outline the sequence of events that compromise the mechanism used by a bacteriophage in order to infect a bacteria cell?arrow_forwardWith respect to F+ and F- bacterial matings, (a) How was it established that physical contact was necessary? (b) How was it established that chromosome transfer was unidirectional? (c) What is the genetic basis of a bacterium being F+?arrow_forwardA transducing particle injects all of the DNA that it contains into a bacterial cell. Which of the following are TRUE? A. The bacterium becomes Hfr B. The bacterium becomes F+ C. The bacterium immediately acquires new functions as the injected DNA is rapidly transcribed and translated into mRNA and proteins D. This injected DNA can integrate into the bacterium’s genome through a process called recombinationarrow_forward
- Compare and contrast the three forms of gene transfer—conjugation, transformation, and transduction—in bacteria.arrow_forwardIn the phage titer experiment, why did you plate multiple dilutions? a. so that you should have a countable plate b. to have duplicates to count c. As a control d. to be certain the E. coli was not previously infectedarrow_forwardQ. The diagram is given below shows conjugation between F + or Hfr donor cells with F-recipient cells. On the basis of this diagram, answer the following questions.1. How you will differentiate between an F + and an Hfr strain? 2. Among two strains, which type of strain do you expect to transfer bacterial genes to F-cells? 3. What may be the end status of both donor and recipient cells of F plasmid-mediated conjugation? 4. What may be the end result when Hfr conjugates with F- cell?arrow_forward
- Draw a diagram/figure to explain the conjugation process (e.g. use PowerPoint or draw one by hand and include a photo of it). You should include in the diagram the F- recipient, Hfr Donor and the transconjugant/recombinant recipient. Make sure to include the genes encoding for Leucine, Threonine, Thiamine and Streptomycin resistance in your diagram. How does an Hfr strain of coli transfers chromosomal DNA to an F- strain? What determines how much of the chromosomal DNA is transferred?arrow_forward. In two isolates (one is resistant to ampicillin and theother is sensitive to ampicillin) of a new bacterium,you found that genes encoding ampicillin resistanceare being transferred into the sensitive strain.a. How would you know that gene transfer is takingplace?arrow_forwardYou mix two auxotrophic strains of bacteria; one is leu+ trp+ his– met– and the other is leu– trp– his+ met+. After mixing the two strains, you plate the bacteria on minimal medium and observe a few prototrophic colonies (leu+ trp+ his+ met+). To determine whether gene transfer has occurred through conjugation, transformation, or transduction, you design an experiment in which you place filters between the two strains to see if gene transfer occurs. You set up three treatments:-Treatment A No filter, allowing contact between the bacterial strains-Treatment B Filter that blocks cells, but allows viruses and DNA through the filter-Treatment C Filter that blocks cells and viruses, but allows DNA through the filter When you check the results of your experiment, you find prototrophic colonies have occurred following treatments A and B, but not following treatment C. You conclude that gene transfer occurred through... Answer Choices Available: A) Conjugation B) Transformation C)…arrow_forward
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