Study Guide And Solutions Manual For Genetic Analysis: An Integrated Approach
3rd Edition
ISBN: 9780134832258
Author: Mark F. Sanders, John L. Bowman, Peter Mirabito
Publisher: PEARSON
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Chapter 6, Problem 2P
The flow diagram identifies relationships between bacterial strains in various F factor states. For each of the four arrows in the diagram, provide a description of the events involved in the transition.
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For bacteria that are F+, Hfr, F', and F- answer the following.
a. Describe the state of the F factor.
b. Which of these cells are donors? Which is the recipient?
c. Which of these donors can convert exconjugants to a donor state?
d. Which of these donors can transfer a donor gene to exconjugants?
e. Describe the results of conjugation (i.e., changes in the recipient and the exconjugant) that allow detection of the state of the F factor in a donor strain.
f. Describe a "partial diploid" and how it originates.
Describe the state of the F factor in an Hfr, F+, andF− strain.
A conjugation-deficient strain of A. radiobacter is used to combat crown gall disease. Explain how this bacterium prevents the disease, and describe the advantage of using a conjugation-deficient strain.
Chapter 6 Solutions
Study Guide And Solutions Manual For Genetic Analysis: An Integrated Approach
Ch. 6 - For bacteria that are F+, Hfr, F', and F-, perform...Ch. 6 - The flow diagram identifies relationships between...Ch. 6 - Conjugation between an Hfr cell and an F-cell does...Ch. 6 - Bacteria transfer genes by conjugation,...Ch. 6 - Explain the importance of the following features...Ch. 6 - Prob. 6PCh. 6 - Describe what is meant by the term site-specific...Ch. 6 - What is a prophage, and how is a prophage formed?Ch. 6 - How is the frequency of cotransduction related to...Ch. 6 - Describe the differences between genetic...
Ch. 6 - Among the mechanisms of gene transfer in bacteria,...Ch. 6 - What is lateral gene transfer? How might it take...Ch. 6 - Lateral gene transfer is thought to have played a...Ch. 6 - Prob. 14PCh. 6 - A 2013 CDC report identified the practice of...Ch. 6 - Hfr strains that differ in integrated F factor...Ch. 6 - Five Hfr strains from the same bacterial species...Ch. 6 - An interrupted mating study is carried out on Hfr...Ch. 6 - An Hfr strain with the genotype cys+leu+met+strS...Ch. 6 - A triple-auxotrophic strain of E. coli having the...Ch. 6 - Penicillin was first used in the 1940 s to treat...Ch. 6 - An attribute of growth behavior of eight...Ch. 6 - Synthesis of the amino acid histidine is a...Ch. 6 - The phage P1 is used as a generalized transducing...Ch. 6 - Prob. 25PCh. 6 - Prob. 26PCh. 6 - Look closely at the consolidated Hfr map and the...Ch. 6 - Fifty bacterial colonies are on a complete-medium...
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- Results from a Kirby Bauer antibiotic assay on a Gram-negative bacterial culture are described as follows: A) the bacterium is resistant to penicillin, an antibiotic that targets synthesis of the peptidoglycan cell wall and B) the bacterium is resistant to tetracycline, an antibiotic that targets the small subunit of the ribosome, inhibiting protein synthesis. Which of the results represents intrinsic resistant and which represents acquired resistants?arrow_forwardIn the Meselson experiment, would it have been possible to utilize N14 in the original bacteria and only allow growing bacteria to access N15 to build new (F1) bacteria and get the same results? Explain why. (I need an answer in full complete sentences with the question restated in the response.)arrow_forwardYou have isolated a strain of E.coli that is resistant to penicillin,streptomycin,chloramphenicol and tetracycline. You also observe that when you mix this strain with a strain that is sensitive to all four antibiotics, the new strain becomes resistant to streptomycin,penicillin and chloramphenicol but not tetracycline. Explain how this is so?arrow_forward
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