Study Guide And Solutions Manual For Genetic Analysis: An Integrated Approach
3rd Edition
ISBN: 9780134832258
Author: Mark F. Sanders, John L. Bowman, Peter Mirabito
Publisher: PEARSON
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Chapter 6, Problem 7P
Describe what is meant by the term site-specific recombination as used in identifying the processes that lead to the integration of temperate bacteriophages into host bacterial chromosomes during lysogeny or to the formation of specialized transducing phage.
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Chapter 6 Solutions
Study Guide And Solutions Manual For Genetic Analysis: An Integrated Approach
Ch. 6 - For bacteria that are F+, Hfr, F', and F-, perform...Ch. 6 - The flow diagram identifies relationships between...Ch. 6 - Conjugation between an Hfr cell and an F-cell does...Ch. 6 - Bacteria transfer genes by conjugation,...Ch. 6 - Explain the importance of the following features...Ch. 6 - Prob. 6PCh. 6 - Describe what is meant by the term site-specific...Ch. 6 - What is a prophage, and how is a prophage formed?Ch. 6 - How is the frequency of cotransduction related to...Ch. 6 - Describe the differences between genetic...
Ch. 6 - Among the mechanisms of gene transfer in bacteria,...Ch. 6 - What is lateral gene transfer? How might it take...Ch. 6 - Lateral gene transfer is thought to have played a...Ch. 6 - Prob. 14PCh. 6 - A 2013 CDC report identified the practice of...Ch. 6 - Hfr strains that differ in integrated F factor...Ch. 6 - Five Hfr strains from the same bacterial species...Ch. 6 - An interrupted mating study is carried out on Hfr...Ch. 6 - An Hfr strain with the genotype cys+leu+met+strS...Ch. 6 - A triple-auxotrophic strain of E. coli having the...Ch. 6 - Penicillin was first used in the 1940 s to treat...Ch. 6 - An attribute of growth behavior of eight...Ch. 6 - Synthesis of the amino acid histidine is a...Ch. 6 - The phage P1 is used as a generalized transducing...Ch. 6 - Prob. 25PCh. 6 - Prob. 26PCh. 6 - Look closely at the consolidated Hfr map and the...Ch. 6 - Fifty bacterial colonies are on a complete-medium...
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- The bacteriophage genome consists primarily of genes encodingproteins that make up the head, collar and tail, and tail fibers When these genes are transcribed following phage infection, howare these proteins synthesized, since the phage genome lacksgenes essential to ribosome structure?arrow_forwardDiscuss the genetic switch that controls the lytic versus lysogenic path in bacteriophage lambda.arrow_forwardTargeting vectors use homologoues recombination to insert transgenes at specific chosen locations in the host genome. Discuss this statement.arrow_forward
- The linear dsDNA genome of λ binds on the LamB receptor of E. Coli and conducts a normal lysogenic cycle. Exposure to stress will cause the excision of λ prophage from the E. Coli genome. The excised λ genome is then replicated, packaged, and released from the cell as mature λ phage particles and ready to infect other bacterial cells. Among λ phage particles,the transducing phage mediates a specific type of recombination. Understand this scenario and answer the following questions. 1. What are the basic requirements for the insertion of λ into the E. Coli genome? 2. What special features are found in the λ insertion site? 3. What type of recombination occurs with λ insertion in the E. Coli genome? 4. How you will differentiate λ transducing phage from normal λ phage? 5. What exclusive mechanism λ phage utilizes for recombination?arrow_forwardIn gene targeting, homologous recombination between thebacterial chromosome and a linear _______- construct synthesized in vitro can generate a null mutation in any gene.arrow_forwardList the components that would have to be introduced to import site-specific recombination into a newly discovered organism.arrow_forward
- Austin Taylor and Edward Adelberg isolated some new strains of Hfr cells that they then used to map several genes in Escherichia coli by using interrupted conjugation. In one experiment, the researchers mixed cells of Hfr strain AB‑312, which were xyl+ mtl+ mal+ met+ and sensitive to phage T6, with F− strain AB‑531, which was xyl− mtl− mal− met− and resistant to phage T6. The cells were allowed to undergo conjugation. At regular intervals, the researchers removed a sample of cells and interrupted conjugation by killing the Hfr cells with phage T6. The F− cells, which were resistant to phage T6, survived and were then tested for the presence of genes transferred from the Hfr strain. The results of this experiment are shown in the graph. On the basis of these data, give the order of the xyl, mtl, mal, and met genes on the bacterial chromosome and the minimum distances between them in minutes. The origin of transfer is represented by the red triangle. The distances between genes are not…arrow_forwardDescribe the process of cloning a DNA fragment into the EcoR1and AluI sites of the vector pUC18arrow_forwardHow does homologous recombination with transfected disruption constructs can inactivate specific target genes in yeast? Explain this with the help of figure.arrow_forward
- By conducting conjugation experiments between Hfr and recipientstrains, Wollman and Jacob mapped the order of many bacterialgenes. Throughout the course of their studies, they identified severaldifferent Hfr strains in which the F-factor DNA had been integratedat different places along the bacterial chromosome. A sample of theirexperimental results is shown in the following table:Explain how these results are consistent with the idea that thebacterial chromosome is circular?arrow_forwardBy conducting conjugation experiments between Hfr and recipientstrains, Wollman and Jacob mapped the order of many bacterialgenes. Throughout the course of their studies, they identified severaldifferent Hfr strains in which the F-factor DNA had been integratedat different places along the bacterial chromosome. A sample of theirexperimental results is shown in the following table:What information do you know based on the question and your understanding of the topic?arrow_forwardConsider the phase variation of flagella in Salmonella. Describe the phenotype of a cell in which hin is deleted. Will it be motile? Will it undergo phase variation? Which flagellin(s) will be used, if any? Name two bacterial defenses against bacteriophages and explain how they work How might a phage evolve resistance to the CRISPR host defense?arrow_forward
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