Study Guide And Solutions Manual For Genetic Analysis: An Integrated Approach
Study Guide And Solutions Manual For Genetic Analysis: An Integrated Approach
3rd Edition
ISBN: 9780134832258
Author: Mark F. Sanders, John L. Bowman, Peter Mirabito
Publisher: PEARSON
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Chapter 6, Problem 3P

Conjugation between an Hfr cell and an F-cell does not usually result in conversion of exconjugants to the donor state. Occasionally however, the result of this conjugation is two Hfr cells. Explain how this occurs.

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Rarely, the conjugation of Hfr and F− cells produces two Hfr cells. Explain how this event takes place.
This question combines your knowledge of bacterial conjugationand the genetic regulation that directs thephage λ reproductive cycles. When researchers mix donor Hfr strains with recipient F– bacteria that are lysogenic for phage λ, the conjugated cells survive normally. However, if donor Hfr strains that are lysogenic for phage λ conjugate with recipient F– bacteria that do not contain any phage λ, the recipient cells often lyse, due to the induction of λ into the lytic cycle. Based on your knowledge of the regulation of the two reproductive cycles of phage λ, explain this experimental observation.
Describe the differences between the F factor and Hfr transfer. Include how the donor and recipient are affected in each type of conjugation.

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Study Guide And Solutions Manual For Genetic Analysis: An Integrated Approach

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