Chapter 6, Problem 28P

Fifty bacterial colonies are on a complete-medium growth plate. The colonies are replica plated to a minimal medium plate, and 46 colonies grow. What can you say about the bacteria from the four colonies that do not grow? Design an experiment and describe the methods you would use to determine if any of these four colonies are ${\text{leu}}^{\text{-}}{\text{,arg}}^{\text{-}}\text{, or}{\text{val}}^{\text{-}}$.