Study Guide And Solutions Manual For Genetic Analysis: An Integrated Approach
Study Guide And Solutions Manual For Genetic Analysis: An Integrated Approach
3rd Edition
ISBN: 9780134832258
Author: Mark F. Sanders, John L. Bowman, Peter Mirabito
Publisher: PEARSON
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Chapter 6, Problem 23P

Synthesis of the amino acid histidine is a multistep anabolic pathway that uses the products of 13 genes (hisAto hisM) in E. coli. Two independently isolated his -E. coli mutants, designated his1- and his2-, are studied in a conjugation experiment. A his+ F' donor strain that carries a copy of the hisJ gene on the plasmid is mated with a his1- recipient strain in experiment 1 and with a his2- recipient in experiment 2 . The exconjugants are grown on plates lacking histidine. Growth is observed among the exconjugants of experiment 2 but not among those of experiment 1 .

a. Why is growth observed in experiment 2 but not in experiment 1 ?

b. What is the genotype of exconjugants in experiment 2?

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Study Guide And Solutions Manual For Genetic Analysis: An Integrated Approach

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