Study Guide And Solutions Manual For Genetic Analysis: An Integrated Approach
3rd Edition
ISBN: 9780134832258
Author: Mark F. Sanders, John L. Bowman, Peter Mirabito
Publisher: PEARSON
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Textbook Question
Chapter 6, Problem 9P
How is the frequency of cotransduction related to the relative positions of genes on a bacterial chromosome? Draw a map of three genes and describe the expected relationship of cotransduction frequencies to the map.
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With respect to F+ and F- bacterial matings, answer the following questions: (a) How was it established that physical contact between cells was necessary? (b) How was it established that chromosome transfer was unidirectional? (c) What is the genetic basis for a bacterium’s being F+?
Three different Hfr donor strains are mixed with separate samples of an F− strain, and the following mapping data are provided from studies of interrupted conjugation: Appearance of genes in F − cells Hfr1: Genes b+ d+ c+ f+ g+ Time* 3 5 16 27 59 Hfr2: Genes e+ f+ c+ d+ b+ Time 6 24 35 46 48 Hfr3: Genes d+ c+ f+ e+ g+ Time 4 15 26 44 58 Construct a genetic map for these genes, indicating their order on the bacterial chromosome and the distances between them.
In gene mapping using generalized transduction, bacterial genes that are cotransduced are
a. far apart on the bacterial chromosome.
b. on different bacterial chromosomes.
c. close together on the bacterial chromosome.
d. on a plasmid.
Chapter 6 Solutions
Study Guide And Solutions Manual For Genetic Analysis: An Integrated Approach
Ch. 6 - For bacteria that are F+, Hfr, F', and F-, perform...Ch. 6 - The flow diagram identifies relationships between...Ch. 6 - Conjugation between an Hfr cell and an F-cell does...Ch. 6 - Bacteria transfer genes by conjugation,...Ch. 6 - Explain the importance of the following features...Ch. 6 - Prob. 6PCh. 6 - Describe what is meant by the term site-specific...Ch. 6 - What is a prophage, and how is a prophage formed?Ch. 6 - How is the frequency of cotransduction related to...Ch. 6 - Describe the differences between genetic...
Ch. 6 - Among the mechanisms of gene transfer in bacteria,...Ch. 6 - What is lateral gene transfer? How might it take...Ch. 6 - Lateral gene transfer is thought to have played a...Ch. 6 - Prob. 14PCh. 6 - A 2013 CDC report identified the practice of...Ch. 6 - Hfr strains that differ in integrated F factor...Ch. 6 - Five Hfr strains from the same bacterial species...Ch. 6 - An interrupted mating study is carried out on Hfr...Ch. 6 - An Hfr strain with the genotype cys+leu+met+strS...Ch. 6 - A triple-auxotrophic strain of E. coli having the...Ch. 6 - Penicillin was first used in the 1940 s to treat...Ch. 6 - An attribute of growth behavior of eight...Ch. 6 - Synthesis of the amino acid histidine is a...Ch. 6 - The phage P1 is used as a generalized transducing...Ch. 6 - Prob. 25PCh. 6 - Prob. 26PCh. 6 - Look closely at the consolidated Hfr map and the...Ch. 6 - Fifty bacterial colonies are on a complete-medium...
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- In a conjugation experiment, what is meant by the time of entry?arrow_forwardWhen the interrupted mating technique was used with five different strains of Hfr bacteria, the following orders of gene entry and recombination were observed. On the basis of these data, draw a map of the bacterial chromosome. Do the data support the concept of circularity?Hfr Strain Order1 T C H R O2 H R O M B3 M O R H C4 M B A K T5 C T K A Barrow_forwardDNA from a strain of Bacillus subtilis with genotype a + b + c + d + e + is used to transform a strain with genotype a – b – c – d – e – . Pairs of genes are checked for co-transformation and results shown below are obtained. Based on these results, what is the order of the genes on the bacterial chromosome? Show how you work out the order by diagraming.arrow_forward
- In Figure , which gene on the bacterial chromosome will be transferred last in strain Hfr5?arrow_forwardBy conducting conjugation experiments between Hfr and recipientstrains, Wollman and Jacob mapped the order of many bacterialgenes. Throughout the course of their studies, they identified severaldifferent Hfr strains in which the F-factor DNA had been integratedat different places along the bacterial chromosome. A sample of theirexperimental results is shown in the table:Draw a map that shows the order of genes and the locations ofthe origins of transfer among these different Hfr strains?arrow_forwardAn Hfr strain that is leuA+ and thiL+ was mixed with a strain thatis leuA− and thiL−. In the data points shown in the following graph,the conjugation was interrupted at different time points, and thepercentage of recombinants for each gene was determined bystreaking on a medium that lacked either leucine or thiamine.What is the map distance (in minutes) between these two genes?arrow_forward
- A donor strain of bacteria with alleles a+ b+ c+ is infected with phages to map the donor chromosome using generalized transduction. The phage lysate from the bacterial cells is collected and used to infect a second strain of bacteria that are a− b− c−. Bacteria with the a+ allele are selected, and the percentage of cells with cotransduced b+ and c+ alleles are recorded. Donor Recipient Selected allele Cells with cotransduced allele (%) a+ b+ c+ a− b− c− a+ 25 b+ a+ 3 c+ Is gene b or gene c closer to gene a? Explain your reasoning.arrow_forwardAs mentioned in question 2 of More Genetic TIPS, origins oftransfer can be located in many different places on a bacterial chromosome,and their direction of transfer can be clockwise or counterclockwise.Let’s suppose a researcher conjugated six differentHfr strains that were thr + leu + tons str r azis lac + gal + pro + met +to an F − strain that was thr − leu − tonr str s azir lac − gal − pro −met −, and obtained the following results: Draw a map of the circular E. coli chromosome that shows thelocations and orientations of the origins of transfer in these six Hfrstrains.arrow_forwardIn the Hershey Chase experiment, why was the localization of radio-labeled phage of macromolecules in the cytoplasm the reason that the genetic material identity was proved.arrow_forward
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genetic recombination strategies of bacteria CONJUGATION, TRANSDUCTION AND TRANSFORMATION; Author: Scientist Cindy;https://www.youtube.com/watch?v=_Va8FZJEl9A;License: Standard youtube license