Organization of domains in the precursor gene for the x light chain V3 Va V300 J2 Ja JAc RAG1 and RAG2 recognize recombination signal sequences and catalyze the breakage at the end of a variable domain and beginning of a joining domain. In this example, it occurs at the end of V7g and beginning of J2. V V2 V3 Va V78 The intervening DNA is lost. NHEJ proteins catalyze the joining of the last V domain and first J domain in V300 the remaining DNA. The gene is transcribed into a pre-MRNA starting at the last variable domain. V78 J2 J3 3' 5' The region between the first joining domain and constant domain in the pre-MRNA is spliced out. 3' V78 J2 The mRNA is translated into a polypeptide containing 1 variable, 1 joining, and 1 constant domain. NH, V78 Vтв Ja -соон 2 light polypeptides and 2 heavy polypeptides assemble to form a functional antibody protein. NH2 NH2 Heavy polypeptide СООН -COOH A functiona antibody -COOH made in 1B cell СООн NH,- `Light polypeptide NH FIGURE 20.7 Site-specific recombination within the precur- sor gene that encodes the x light chain for immunoglobulin G (IgG) in the mouse.

Organization of domains in the precursor gene for the x light chain V3 Va V300 J2 Ja JAc RAG1 and RAG2 recognize recombination signal sequences and catalyze the breakage at the end of a variable domain and beginning of a joining domain. In this example, it occurs at the end of V7g and beginning of J2. V V2 V3 Va V78 The intervening DNA is lost. NHEJ proteins catalyze the joining of the last V domain and first J domain in V300 the remaining DNA. The gene is transcribed into a pre-MRNA starting at the last variable domain. V78 J2 J3 3' 5' The region between the first joining domain and constant domain in the pre-MRNA is spliced out. 3' V78 J2 The mRNA is translated into a polypeptide containing 1 variable, 1 joining, and 1 constant domain. NH, V78 Vтв Ja -соон 2 light polypeptides and 2 heavy polypeptides assemble to form a functional antibody protein. NH2 NH2 Heavy polypeptide СООН -COOH A functiona antibody -COOH made in 1B cell СООн NH,- `Light polypeptide NH FIGURE 20.7 Site-specific recombination within the precur- sor gene that encodes the x light chain for immunoglobulin G (IgG) in the mouse.

Name: According to the scenario shown, how many segments of DNA (one, two, o
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Description: According to the scenario shown, how many segments of DNA (one, two, or three) are removed during site-specific recombination within the gene that encodes the κ (kappa) light chain for IgG proteins? How many segments are spliced out of the pre-mRNA?

Biology Today and Tomorrow without Physiology (MindTap Course List)

5th Edition

ISBN:9781305117396

Author:Cecie Starr, Christine Evers, Lisa Starr

Publisher:Cecie Starr, Christine Evers, Lisa Starr

Chapter7: Gene Expression And Control

Section: Chapter Questions

Problem 3DID

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